Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks
Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes watery diarrhea and induces proinflammatory cytokine responses in piglets. Our previous research showed that the specific-pathogen-free (SPF) chicks exhibited mild diarrhea and low fecal viral shedding, along...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00897/full |
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| author | Hai-yan Li Hong-lei Zhang Hong-lei Zhang Fu-jie zhao Fu-jie zhao Shi-qiong Wang Zhi-xiang Wang Zhan-yong Wei Zhan-yong Wei |
| author_facet | Hai-yan Li Hong-lei Zhang Hong-lei Zhang Fu-jie zhao Fu-jie zhao Shi-qiong Wang Zhi-xiang Wang Zhan-yong Wei Zhan-yong Wei |
| author_sort | Hai-yan Li |
| collection | DOAJ |
| description | Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes watery diarrhea and induces proinflammatory cytokine responses in piglets. Our previous research showed that the specific-pathogen-free (SPF) chicks exhibited mild diarrhea and low fecal viral shedding, along with cecum lesions after PDCoV infection. Disturbances in the homeostasis of the gut microbiota have been associated with various diseases. We aimed to explore the effects of PDCoV infection on chick gut microbiota, short-chain fatty acid (SCFAs) production, and inflammatory cytokine expression in chicks, and also to investigate the relationship between gut microbiota and SCFAs or inflammatory cytokine expression of the PDCoV-infected chicks. Results obtained using 16S rRNA sequencing showed that infection with PDCoV strain HNZK-02 significantly altered the composition of chick gut microbiota, with the reduced abundance of Eisenbergiella and Anaerotruncus genera at 5 days post-inoculation (dpi) (P < 0.05), and an increased abundance of Alistipes genus at 17 dpi (P < 0.05). The production of SCFAs in the cecum of PDCoV HNZK-02–infected chicks, including acetic acid, propionic acid, and butyric acid, decreased in all cases. The expression of inflammatory cytokines (interferon-γ, tumor necrosis factor-α, and interleukin-10) was increased in the cecum tissue and serum of the PDCoV HNZK-02–infected chicks when detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Further analysis showed significant correlation between bacterial genera and SCFAs or inflammatory cytokines expression in cecum of the PDCoV infected chicks. These findings might provide new insight into the pathology and physiology of PDCoV in chicks. |
| first_indexed | 2024-12-11T21:44:04Z |
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| id | doaj.art-aa0ee5b5bea54b68b3d9b93d7bb733de |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T21:44:04Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aa0ee5b5bea54b68b3d9b93d7bb733de2022-12-22T00:49:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.00897522169Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected ChicksHai-yan Li0Hong-lei Zhang1Hong-lei Zhang2Fu-jie zhao3Fu-jie zhao4Shi-qiong Wang5Zhi-xiang Wang6Zhan-yong Wei7Zhan-yong Wei8College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaKey Laboratory for Animal-Derived Food Safety of Henan Province, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaKey Laboratory for Animal-Derived Food Safety of Henan Province, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaKey Laboratory for Animal-Derived Food Safety of Henan Province, Henan Agricultural University, Zhengzhou, ChinaPorcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that causes watery diarrhea and induces proinflammatory cytokine responses in piglets. Our previous research showed that the specific-pathogen-free (SPF) chicks exhibited mild diarrhea and low fecal viral shedding, along with cecum lesions after PDCoV infection. Disturbances in the homeostasis of the gut microbiota have been associated with various diseases. We aimed to explore the effects of PDCoV infection on chick gut microbiota, short-chain fatty acid (SCFAs) production, and inflammatory cytokine expression in chicks, and also to investigate the relationship between gut microbiota and SCFAs or inflammatory cytokine expression of the PDCoV-infected chicks. Results obtained using 16S rRNA sequencing showed that infection with PDCoV strain HNZK-02 significantly altered the composition of chick gut microbiota, with the reduced abundance of Eisenbergiella and Anaerotruncus genera at 5 days post-inoculation (dpi) (P < 0.05), and an increased abundance of Alistipes genus at 17 dpi (P < 0.05). The production of SCFAs in the cecum of PDCoV HNZK-02–infected chicks, including acetic acid, propionic acid, and butyric acid, decreased in all cases. The expression of inflammatory cytokines (interferon-γ, tumor necrosis factor-α, and interleukin-10) was increased in the cecum tissue and serum of the PDCoV HNZK-02–infected chicks when detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Further analysis showed significant correlation between bacterial genera and SCFAs or inflammatory cytokines expression in cecum of the PDCoV infected chicks. These findings might provide new insight into the pathology and physiology of PDCoV in chicks.https://www.frontiersin.org/article/10.3389/fmicb.2020.00897/fullPorcine deltacoronavirus (PDCoV)SPF chickintestinal microbiotainflammatory cytokinesshort-chain fatty acids |
| spellingShingle | Hai-yan Li Hong-lei Zhang Hong-lei Zhang Fu-jie zhao Fu-jie zhao Shi-qiong Wang Zhi-xiang Wang Zhan-yong Wei Zhan-yong Wei Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks Frontiers in Microbiology Porcine deltacoronavirus (PDCoV) SPF chick intestinal microbiota inflammatory cytokines short-chain fatty acids |
| title | Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks |
| title_full | Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks |
| title_fullStr | Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks |
| title_full_unstemmed | Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks |
| title_short | Modulation of Gut Microbiota, Short-Chain Fatty Acid Production, and Inflammatory Cytokine Expression in the Cecum of Porcine Deltacoronavirus-Infected Chicks |
| title_sort | modulation of gut microbiota short chain fatty acid production and inflammatory cytokine expression in the cecum of porcine deltacoronavirus infected chicks |
| topic | Porcine deltacoronavirus (PDCoV) SPF chick intestinal microbiota inflammatory cytokines short-chain fatty acids |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00897/full |
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