Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive
In nature, barnacles and bacterial biofilms utilize self-assembly amyloid to achieve strong and robust interface adhesion. However, there is still a lack of sufficient research on the construction of macroscopic adhesives based on amyloid-like nanostructures through reasonable molecular design. Here...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422000540 |
| _version_ | 1811244524789301248 |
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| author | Fei Li Luona Ye Longyu Zhang Xiaoyan Li Xiaoxiao Liu Jiarui Zhu Huanhuan Li Huimin Pang Yunjun Yan Li Xu Min Yang Jinyong Yan |
| author_facet | Fei Li Luona Ye Longyu Zhang Xiaoyan Li Xiaoxiao Liu Jiarui Zhu Huanhuan Li Huimin Pang Yunjun Yan Li Xu Min Yang Jinyong Yan |
| author_sort | Fei Li |
| collection | DOAJ |
| description | In nature, barnacles and bacterial biofilms utilize self-assembly amyloid to achieve strong and robust interface adhesion. However, there is still a lack of sufficient research on the construction of macroscopic adhesives based on amyloid-like nanostructures through reasonable molecular design. Here, we report a genetically programmed self-assembly living-cell bioadhesive inspired by barnacle and curli system. Firstly, the encoding genes of two natural adhesion proteins (CsgA and cp19k) derived from E. coli curli and barnacle cement were fused and expressed as a fundamental building block of the bioadhesive. Utilizing the natural curli system of E. coli, fusion protein can be delivered to cell surface and self-assemble into an amyloid nanofibrous network. Then, the E. coli cells were incorporated into the molecular chain network of xanthan gum (XG) through covalent conjugation to produce a living-cell bioadhesive. The shear adhesive strength of the bioadhesive to the surface of the aluminum sheet reaches 278 kPa. Benefiting from living cells encapsulated inside, the bioadhesive can self-regenerate with adequate nutrients. This adhesive has low toxicity to organisms, strong resistance to the liquid environment in vivo, easy to pump, exhibiting potential application prospects in biomedical fields such as intestinal soft tissue repair. |
| first_indexed | 2024-04-12T14:26:36Z |
| format | Article |
| id | doaj.art-aa14db67b0184c1da9ecfca621a02188 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-12T14:26:36Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-aa14db67b0184c1da9ecfca621a021882022-12-22T03:29:26ZengElsevierMaterials Today Bio2590-00642022-03-0114100256Design of a genetically programmed barnacle-curli inspired living-cell bioadhesiveFei Li0Luona Ye1Longyu Zhang2Xiaoyan Li3Xiaoxiao Liu4Jiarui Zhu5Huanhuan Li6Huimin Pang7Yunjun Yan8Li Xu9Min Yang10Jinyong Yan11Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaCorresponding author.; Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaKey Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaCorresponding author.; Key Lab of Molecular Biophysics of Ministry of Education, College of Life Science and Technology Huazhong University of Science and Technology, Wuhan, ChinaIn nature, barnacles and bacterial biofilms utilize self-assembly amyloid to achieve strong and robust interface adhesion. However, there is still a lack of sufficient research on the construction of macroscopic adhesives based on amyloid-like nanostructures through reasonable molecular design. Here, we report a genetically programmed self-assembly living-cell bioadhesive inspired by barnacle and curli system. Firstly, the encoding genes of two natural adhesion proteins (CsgA and cp19k) derived from E. coli curli and barnacle cement were fused and expressed as a fundamental building block of the bioadhesive. Utilizing the natural curli system of E. coli, fusion protein can be delivered to cell surface and self-assemble into an amyloid nanofibrous network. Then, the E. coli cells were incorporated into the molecular chain network of xanthan gum (XG) through covalent conjugation to produce a living-cell bioadhesive. The shear adhesive strength of the bioadhesive to the surface of the aluminum sheet reaches 278 kPa. Benefiting from living cells encapsulated inside, the bioadhesive can self-regenerate with adequate nutrients. This adhesive has low toxicity to organisms, strong resistance to the liquid environment in vivo, easy to pump, exhibiting potential application prospects in biomedical fields such as intestinal soft tissue repair.http://www.sciencedirect.com/science/article/pii/S2590006422000540BarnaclesBiofilmsSelf-assemblyGenetic engineeringBioadhesives |
| spellingShingle | Fei Li Luona Ye Longyu Zhang Xiaoyan Li Xiaoxiao Liu Jiarui Zhu Huanhuan Li Huimin Pang Yunjun Yan Li Xu Min Yang Jinyong Yan Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive Materials Today Bio Barnacles Biofilms Self-assembly Genetic engineering Bioadhesives |
| title | Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive |
| title_full | Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive |
| title_fullStr | Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive |
| title_full_unstemmed | Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive |
| title_short | Design of a genetically programmed barnacle-curli inspired living-cell bioadhesive |
| title_sort | design of a genetically programmed barnacle curli inspired living cell bioadhesive |
| topic | Barnacles Biofilms Self-assembly Genetic engineering Bioadhesives |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422000540 |
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