Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats
This study was aimed at screening a potential anti-hyperuricemia agent with dual xanthine oxidase (XOD) inhibitory effect (XOI) and antioxidant activity from edible plants with health-promoting effects using non-targeted chemometric analysis (hierarchical cluster analysis and principal component ana...
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| Format: | Article |
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Elsevier
2018-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618305000 |
| _version_ | 1829508642251997184 |
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| author | Lianzhu Lin Xuemei Liu Mouming Zhao |
| author_facet | Lianzhu Lin Xuemei Liu Mouming Zhao |
| author_sort | Lianzhu Lin |
| collection | DOAJ |
| description | This study was aimed at screening a potential anti-hyperuricemia agent with dual xanthine oxidase (XOD) inhibitory effect (XOI) and antioxidant activity from edible plants with health-promoting effects using non-targeted chemometric analysis (hierarchical cluster analysis and principal component analysis), evaluating its in vivo hypouricemic effect and mechanism, and identifying the key XOD inhibitor using ultrafiltration UPLC-MS/MS. Rhizoma Alpiniae Officinarum extract (GE), which has high contents of total phenolics and flavonoids, as well as dual XOI and antioxidant activity, showed significant hypouricemic and renal protective effects on hyperuricemic rats through inhibiting XOD activity, alleviating oxidative stress, down-regulating URAT1 and GLUT9 protein expression, and reducing urea nitrogen and creatinine levels. GE could be a dual-acting agent with XOI and uricosuric effect for preventing and treating hyperuricemia. Four flavonoids and eight diarylheptanoids were identified as potential XOD inhibitors from GE. Galangin, kaempferide and 3-methoxyl-galangin contributing to 70.1% of XOI were the key XOD inhibitors. |
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| format | Article |
| id | doaj.art-aa1a9999a2fb4ff3b312757c1f04b3dd |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T11:30:29Z |
| publishDate | 2018-11-01 |
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| series | Journal of Functional Foods |
| spelling | doaj.art-aa1a9999a2fb4ff3b312757c1f04b3dd2022-12-21T22:33:15ZengElsevierJournal of Functional Foods1756-46462018-11-01502636Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic ratsLianzhu Lin0Xuemei Liu1Mouming Zhao2School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, ChinaSchool of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, ChinaCorresponding author at: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.; School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China; Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, ChinaThis study was aimed at screening a potential anti-hyperuricemia agent with dual xanthine oxidase (XOD) inhibitory effect (XOI) and antioxidant activity from edible plants with health-promoting effects using non-targeted chemometric analysis (hierarchical cluster analysis and principal component analysis), evaluating its in vivo hypouricemic effect and mechanism, and identifying the key XOD inhibitor using ultrafiltration UPLC-MS/MS. Rhizoma Alpiniae Officinarum extract (GE), which has high contents of total phenolics and flavonoids, as well as dual XOI and antioxidant activity, showed significant hypouricemic and renal protective effects on hyperuricemic rats through inhibiting XOD activity, alleviating oxidative stress, down-regulating URAT1 and GLUT9 protein expression, and reducing urea nitrogen and creatinine levels. GE could be a dual-acting agent with XOI and uricosuric effect for preventing and treating hyperuricemia. Four flavonoids and eight diarylheptanoids were identified as potential XOD inhibitors from GE. Galangin, kaempferide and 3-methoxyl-galangin contributing to 70.1% of XOI were the key XOD inhibitors.http://www.sciencedirect.com/science/article/pii/S1756464618305000Edible plants with health-promoting effectsXanthine oxidase inhibitory effectHypouricemic effectRhizoma Alpiniae OfficinarumFlavonoidsDiarylheptanoids |
| spellingShingle | Lianzhu Lin Xuemei Liu Mouming Zhao Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats Journal of Functional Foods Edible plants with health-promoting effects Xanthine oxidase inhibitory effect Hypouricemic effect Rhizoma Alpiniae Officinarum Flavonoids Diarylheptanoids |
| title | Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats |
| title_full | Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats |
| title_fullStr | Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats |
| title_full_unstemmed | Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats |
| title_short | Screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of Rhizoma Alpiniae Officinarum extract on hyperuricemic rats |
| title_sort | screening of xanthine oxidase inhibitor from selected edible plants and hypouricemic effect of rhizoma alpiniae officinarum extract on hyperuricemic rats |
| topic | Edible plants with health-promoting effects Xanthine oxidase inhibitory effect Hypouricemic effect Rhizoma Alpiniae Officinarum Flavonoids Diarylheptanoids |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618305000 |
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