Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery
In recent years, hydrogels have been widely used as drug carriers, especially in the area of protein delivery. The natural silk fibroin produced from cocoons of the Bombyx mori silkworm possesses excellent biocompatibility, significant bioactivity, and biodegradability. Therefore, silk fibroin-based...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/11/3418 |
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| author | Junwei Liu Haowen Sun Yuwei Peng Ligen Chen Wei Xu Rong Shao |
| author_facet | Junwei Liu Haowen Sun Yuwei Peng Ligen Chen Wei Xu Rong Shao |
| author_sort | Junwei Liu |
| collection | DOAJ |
| description | In recent years, hydrogels have been widely used as drug carriers, especially in the area of protein delivery. The natural silk fibroin produced from cocoons of the Bombyx mori silkworm possesses excellent biocompatibility, significant bioactivity, and biodegradability. Therefore, silk fibroin-based hydrogels are arousing widespread interest in biomedical research. In this study, a process for extracting natural silk fibroin from raw silk textile yarns was established, and three aqueous solutions of silk fibroin with different molecular weight distributions were successfully prepared by controlling the degumming time. Silk fibroin was dispersed in the aqueous solution as “spherical” aggregate particles, and the smaller particles continuously accumulated into large particles. Finally, a silk fibroin hydrogel network was formed. A rheological analysis showed that as the concentration of the silk fibroin hydrogel increased its storage modulus increased significantly. The degradation behavior of silk fibroin hydrogel in different media verified its excellent stability, and the prepared silk fibroin hydrogel had good biocompatibility and an excellent drug-loading capacity. After the protein model drug BSA was loaded, the cumulative drug release within 12 h reached 80%. We hope that these investigations will promote the potential utilities of silk fibroin hydrogels in clinical medicine. |
| first_indexed | 2024-03-10T01:04:44Z |
| format | Article |
| id | doaj.art-aa1b37d79d2445e399331e679608d7c2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T01:04:44Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-aa1b37d79d2445e399331e679608d7c22023-11-23T14:28:26ZengMDPI AGMolecules1420-30492022-05-012711341810.3390/molecules27113418Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug DeliveryJunwei Liu0Haowen Sun1Yuwei Peng2Ligen Chen3Wei Xu4Rong Shao5School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng 224051, ChinaSchool of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, ChinaSchool of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, ChinaSchool of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, ChinaSchool of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, ChinaSchool of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng 224051, ChinaIn recent years, hydrogels have been widely used as drug carriers, especially in the area of protein delivery. The natural silk fibroin produced from cocoons of the Bombyx mori silkworm possesses excellent biocompatibility, significant bioactivity, and biodegradability. Therefore, silk fibroin-based hydrogels are arousing widespread interest in biomedical research. In this study, a process for extracting natural silk fibroin from raw silk textile yarns was established, and three aqueous solutions of silk fibroin with different molecular weight distributions were successfully prepared by controlling the degumming time. Silk fibroin was dispersed in the aqueous solution as “spherical” aggregate particles, and the smaller particles continuously accumulated into large particles. Finally, a silk fibroin hydrogel network was formed. A rheological analysis showed that as the concentration of the silk fibroin hydrogel increased its storage modulus increased significantly. The degradation behavior of silk fibroin hydrogel in different media verified its excellent stability, and the prepared silk fibroin hydrogel had good biocompatibility and an excellent drug-loading capacity. After the protein model drug BSA was loaded, the cumulative drug release within 12 h reached 80%. We hope that these investigations will promote the potential utilities of silk fibroin hydrogels in clinical medicine.https://www.mdpi.com/1420-3049/27/11/3418silk fibroinhydrogelrecycled materialdrug delivery |
| spellingShingle | Junwei Liu Haowen Sun Yuwei Peng Ligen Chen Wei Xu Rong Shao Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery Molecules silk fibroin hydrogel recycled material drug delivery |
| title | Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery |
| title_full | Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery |
| title_fullStr | Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery |
| title_full_unstemmed | Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery |
| title_short | Preparation and Characterization of Natural Silk Fibroin Hydrogel for Protein Drug Delivery |
| title_sort | preparation and characterization of natural silk fibroin hydrogel for protein drug delivery |
| topic | silk fibroin hydrogel recycled material drug delivery |
| url | https://www.mdpi.com/1420-3049/27/11/3418 |
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