Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus
Hemocyanin is a critical oxygen-transporting protein exhibiting multiple physiological functions, including protein storage and transportation, osmolarity regulation, molting, and immunomodulation in arthropods and mollusks. However, the immune function of hemocyanin in Portunus trituberculatus is r...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423004027 |
| _version_ | 1827582085265620992 |
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| author | Dongfang Sun Yukun Li Jianjian Lv Junyang Guo Ping Liu Baoquan Gao |
| author_facet | Dongfang Sun Yukun Li Jianjian Lv Junyang Guo Ping Liu Baoquan Gao |
| author_sort | Dongfang Sun |
| collection | DOAJ |
| description | Hemocyanin is a critical oxygen-transporting protein exhibiting multiple physiological functions, including protein storage and transportation, osmolarity regulation, molting, and immunomodulation in arthropods and mollusks. However, the immune function of hemocyanin in Portunus trituberculatus is rarely reported. Here, we identified ten subunit genes within the hemocyanin family from the P. trituberculatus genome. These genes exhibited conserved functional domains and copper ion binding sites. Building upon prior research, we specifically focused on investigating the immune functions of PtHc1 and PtHc2. Tissue expression analysis revealed that PtHc1 and PtHc2 were prominently expressed in the hepatopancreas and hemocytes. Upon infection with Vibrio parahaemolyticus, both PtHc1 and PtHc2 demonstrated a similar expression pattern, characterized by a gradual upregulation of expression levels in the hepatopancreas. While within the hemocytes, they exhibited a trend of initial downregulation followed by subsequent upregulation in expression. In addition, Recombinant hemocyanin was able to inhibit bacterial proliferation in a dose-dependent manner, with inhibition rates of 40 % and 39 % when the recombinant proteins of PtHc1 and PtHc2 reached 0.03 mg/mL, respectively. Besides, V. parahaemolyticus challenge resulted in a significant 20–23 % increase in mortality in P. trituberculatus following knockdown of PtHc1 and PtHc2. Overall, our study sheds light on the immune-related functions of PtHc1 and PtHc2 in P. trituberculatus, revealing their potential as key players in defending against pathogenic threats like V. parahaemolyticus. These results enriched the field of innate immunity in crustacean and providing a theoretical basis for disease resistance breeding. |
| first_indexed | 2024-03-08T22:45:29Z |
| format | Article |
| id | doaj.art-aa1df6b2a07746efb06f3fed4d4e4772 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:45:29Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-aa1df6b2a07746efb06f3fed4d4e47722023-12-17T06:40:01ZengElsevierAquaculture Reports2352-51342023-12-0133101863Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatusDongfang Sun0Yukun Li1Jianjian Lv2Junyang Guo3Ping Liu4Baoquan Gao5National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; Corresponding author at: National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.Hemocyanin is a critical oxygen-transporting protein exhibiting multiple physiological functions, including protein storage and transportation, osmolarity regulation, molting, and immunomodulation in arthropods and mollusks. However, the immune function of hemocyanin in Portunus trituberculatus is rarely reported. Here, we identified ten subunit genes within the hemocyanin family from the P. trituberculatus genome. These genes exhibited conserved functional domains and copper ion binding sites. Building upon prior research, we specifically focused on investigating the immune functions of PtHc1 and PtHc2. Tissue expression analysis revealed that PtHc1 and PtHc2 were prominently expressed in the hepatopancreas and hemocytes. Upon infection with Vibrio parahaemolyticus, both PtHc1 and PtHc2 demonstrated a similar expression pattern, characterized by a gradual upregulation of expression levels in the hepatopancreas. While within the hemocytes, they exhibited a trend of initial downregulation followed by subsequent upregulation in expression. In addition, Recombinant hemocyanin was able to inhibit bacterial proliferation in a dose-dependent manner, with inhibition rates of 40 % and 39 % when the recombinant proteins of PtHc1 and PtHc2 reached 0.03 mg/mL, respectively. Besides, V. parahaemolyticus challenge resulted in a significant 20–23 % increase in mortality in P. trituberculatus following knockdown of PtHc1 and PtHc2. Overall, our study sheds light on the immune-related functions of PtHc1 and PtHc2 in P. trituberculatus, revealing their potential as key players in defending against pathogenic threats like V. parahaemolyticus. These results enriched the field of innate immunity in crustacean and providing a theoretical basis for disease resistance breeding.http://www.sciencedirect.com/science/article/pii/S2352513423004027Portunus trituberculatusHemocyaninVibrio parahaemolyticusCrustacean |
| spellingShingle | Dongfang Sun Yukun Li Jianjian Lv Junyang Guo Ping Liu Baoquan Gao Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus Aquaculture Reports Portunus trituberculatus Hemocyanin Vibrio parahaemolyticus Crustacean |
| title | Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus |
| title_full | Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus |
| title_fullStr | Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus |
| title_full_unstemmed | Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus |
| title_short | Response pattern of hemocyanin to Vibrio parahaemolyticus infection and its immune function in the swimming crab Portunus trituberculatus |
| title_sort | response pattern of hemocyanin to vibrio parahaemolyticus infection and its immune function in the swimming crab portunus trituberculatus |
| topic | Portunus trituberculatus Hemocyanin Vibrio parahaemolyticus Crustacean |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423004027 |
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