Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets.
DNA logic circuits utilizing DNA hybridization and/or enzymatic reactions have drawn increasing attention for their potential applications in the diagnosis and treatment of cellular diseases. The compartmentalization of such a system into a microdroplet considerably helps to precisely regulate local...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5507272?pdf=render |
| _version_ | 1818362833289609216 |
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| author | Hiroki Yasuga Kosuke Inoue Ryuji Kawano Masahiro Takinoue Toshihisa Osaki Koki Kamiya Norihisa Miki Shoji Takeuchi |
| author_facet | Hiroki Yasuga Kosuke Inoue Ryuji Kawano Masahiro Takinoue Toshihisa Osaki Koki Kamiya Norihisa Miki Shoji Takeuchi |
| author_sort | Hiroki Yasuga |
| collection | DOAJ |
| description | DNA logic circuits utilizing DNA hybridization and/or enzymatic reactions have drawn increasing attention for their potential applications in the diagnosis and treatment of cellular diseases. The compartmentalization of such a system into a microdroplet considerably helps to precisely regulate local interactions and reactions between molecules. In this study, we introduced a relay approach for enabling the transfer of DNA from one droplet to another to implement multi-step sequential logic operations. We proposed electrical fusion and mechanical splitting of droplets to facilitate the DNA flow at the inputs, logic operation, output, and serial connection between two logic gates. We developed Negative-OR operations integrated by a serial connection of the OR gate and NOT gate incorporated in a series of droplets. The four types of input defined by the presence/absence of DNA in the input droplet pair were correctly reflected in the readout at the Negative-OR gate. The proposed approach potentially allows for serial and parallel logic operations that could be used for complex diagnostic applications. |
| first_indexed | 2024-12-13T21:38:51Z |
| format | Article |
| id | doaj.art-aa245e59b6d44b7b8f9196a1770da6e0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:38:51Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-aa245e59b6d44b7b8f9196a1770da6e02022-12-21T23:30:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018087610.1371/journal.pone.0180876Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets.Hiroki YasugaKosuke InoueRyuji KawanoMasahiro TakinoueToshihisa OsakiKoki KamiyaNorihisa MikiShoji TakeuchiDNA logic circuits utilizing DNA hybridization and/or enzymatic reactions have drawn increasing attention for their potential applications in the diagnosis and treatment of cellular diseases. The compartmentalization of such a system into a microdroplet considerably helps to precisely regulate local interactions and reactions between molecules. In this study, we introduced a relay approach for enabling the transfer of DNA from one droplet to another to implement multi-step sequential logic operations. We proposed electrical fusion and mechanical splitting of droplets to facilitate the DNA flow at the inputs, logic operation, output, and serial connection between two logic gates. We developed Negative-OR operations integrated by a serial connection of the OR gate and NOT gate incorporated in a series of droplets. The four types of input defined by the presence/absence of DNA in the input droplet pair were correctly reflected in the readout at the Negative-OR gate. The proposed approach potentially allows for serial and parallel logic operations that could be used for complex diagnostic applications.http://europepmc.org/articles/PMC5507272?pdf=render |
| spellingShingle | Hiroki Yasuga Kosuke Inoue Ryuji Kawano Masahiro Takinoue Toshihisa Osaki Koki Kamiya Norihisa Miki Shoji Takeuchi Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. PLoS ONE |
| title | Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. |
| title_full | Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. |
| title_fullStr | Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. |
| title_full_unstemmed | Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. |
| title_short | Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. |
| title_sort | serial dna relay in dna logic gates by electrical fusion and mechanical splitting of droplets |
| url | http://europepmc.org/articles/PMC5507272?pdf=render |
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