Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates
Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly lo...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717301201 |
| _version_ | 1818295806836342784 |
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| author | Medya Mara Shikhagaie Åsa K. Björklund Jenny Mjösberg Jonas S. Erjefält Anne S. Cornelissen Xavier Romero Ros Suzanne M. Bal Jasper J. Koning Reina E. Mebius Michiko Mori Melanie Bruchard Bianca Blom Hergen Spits |
| author_facet | Medya Mara Shikhagaie Åsa K. Björklund Jenny Mjösberg Jonas S. Erjefält Anne S. Cornelissen Xavier Romero Ros Suzanne M. Bal Jasper J. Koning Reina E. Mebius Michiko Mori Melanie Bruchard Bianca Blom Hergen Spits |
| author_sort | Medya Mara Shikhagaie |
| collection | DOAJ |
| description | Here, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates. |
| first_indexed | 2024-12-13T03:53:30Z |
| format | Article |
| id | doaj.art-aa25019afd43430bba29f05387ad8fb5 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-13T03:53:30Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-aa25019afd43430bba29f05387ad8fb52022-12-22T00:00:40ZengElsevierCell Reports2211-12472017-02-011871761177310.1016/j.celrep.2017.01.063Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid AggregatesMedya Mara Shikhagaie0Åsa K. Björklund1Jenny Mjösberg2Jonas S. Erjefält3Anne S. Cornelissen4Xavier Romero Ros5Suzanne M. Bal6Jasper J. Koning7Reina E. Mebius8Michiko Mori9Melanie Bruchard10Bianca Blom11Hergen Spits12Department of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, SwedenCenter for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, 171 77 Stockholm, SwedenUnit of Airway Inflammation, Department of Experimental Medical Sciences, Lund University, 221 84 Lund, SwedenDepartment of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, 1006 AN Amsterdam, the NetherlandsDepartment of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 HV Amsterdam, the NetherlandsDepartment of Molecular Cell Biology and Immunology, VU University Medical Center, 1081 HV Amsterdam, the NetherlandsUnit of Airway Inflammation, Department of Experimental Medical Sciences, Lund University, 221 84 Lund, SwedenDepartment of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Experimental Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsHere, we characterize a subset of ILC3s that express Neuropilin1 (NRP1) and are present in lymphoid tissues, but not in the peripheral blood or skin. NRP1+ group 3 innate lymphoid cells (ILC3s) display in vitro lymphoid tissue inducer (LTi) activity. In agreement with this, NRP1+ ILC3s are mainly located in proximity to high endothelial venules (HEVs) and express cell surface molecules involved in lymphocyte migration in secondary lymphoid tissues via HEVs. NRP1 was also expressed on mouse fetal LTi cells, indicating that NRP1 is a conserved marker for LTi cells. Human NRP1+ ILC3s are primed cells because they express CD45RO and produce higher amounts of cytokines than NRP1− cells, which express CD45RA. The NRP1 ligand vascular endothelial growth factor A (VEGF-A) served as a chemotactic factor for NRP1+ ILC3s. NRP1+ ILC3s are present in lung tissues from smokers and patients with chronic obstructive pulmonary disease, suggesting a role in angiogenesis and/or the initiation of ectopic pulmonary lymphoid aggregates.http://www.sciencedirect.com/science/article/pii/S2211124717301201neuropilin-1NRP1lymphoid tissue inducer cellsLTihuman innate lymphoid cells 3ILC3high endothelial venuesHEVsvascular endothelial growth factor AVEGF-Achemotaxissmoke-induced pulmonary lymphoid aggregatesLA |
| spellingShingle | Medya Mara Shikhagaie Åsa K. Björklund Jenny Mjösberg Jonas S. Erjefält Anne S. Cornelissen Xavier Romero Ros Suzanne M. Bal Jasper J. Koning Reina E. Mebius Michiko Mori Melanie Bruchard Bianca Blom Hergen Spits Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates Cell Reports neuropilin-1 NRP1 lymphoid tissue inducer cells LTi human innate lymphoid cells 3 ILC3 high endothelial venues HEVs vascular endothelial growth factor A VEGF-A chemotaxis smoke-induced pulmonary lymphoid aggregates LA |
| title | Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates |
| title_full | Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates |
| title_fullStr | Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates |
| title_full_unstemmed | Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates |
| title_short | Neuropilin-1 Is Expressed on Lymphoid Tissue Residing LTi-like Group 3 Innate Lymphoid Cells and Associated with Ectopic Lymphoid Aggregates |
| title_sort | neuropilin 1 is expressed on lymphoid tissue residing lti like group 3 innate lymphoid cells and associated with ectopic lymphoid aggregates |
| topic | neuropilin-1 NRP1 lymphoid tissue inducer cells LTi human innate lymphoid cells 3 ILC3 high endothelial venues HEVs vascular endothelial growth factor A VEGF-A chemotaxis smoke-induced pulmonary lymphoid aggregates LA |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717301201 |
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