Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析)
采用PCR方法扩增了嗜盐古菌AB19、AB30和AJ5编码螺旋C至螺旋G的氯视紫红质(halorhodopsin，HR)蛋白基因片断和16S rRNA基因(16S rDNA)，测定了它们的核苷酸序列，与已报道的hr基因序列差异显著.获得AJ5 HR蛋白基因序列在Halobiforma属中尚属首次.对hr基因序列进行的遗传分析，包括GC含量、转换与颠换的比值、密码子使用偏好，表明hr基因面临进化选择和偏倚突变压的双重制约，是一类进化程度较高的基因.对比br基因编码蛋白螺旋C到G的序列发现它们具有相似的种属特征.采用BR和HR蛋白螺旋C到G序列构建系统发生树比传统的16S rDNA序列具有优势，B...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2008-01-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
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| Online Access: | https://doi.org/zjup/1008-9497.2008.35.1.80-86 |
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| author | SHIHan(时晗) WUMin(吴敏) XUXue-wei(许学伟) ZHOUZhen(周祯) |
| author_facet | SHIHan(时晗) WUMin(吴敏) XUXue-wei(许学伟) ZHOUZhen(周祯) |
| author_sort | SHIHan(时晗) |
| collection | DOAJ |
| description | 采用PCR方法扩增了嗜盐古菌AB19、AB30和AJ5编码螺旋C至螺旋G的氯视紫红质(halorhodopsin，HR)蛋白基因片断和16S rRNA基因(16S rDNA)，测定了它们的核苷酸序列，与已报道的hr基因序列差异显著.获得AJ5 HR蛋白基因序列在Halobiforma属中尚属首次.对hr基因序列进行的遗传分析，包括GC含量、转换与颠换的比值、密码子使用偏好，表明hr基因面临进化选择和偏倚突变压的双重制约，是一类进化程度较高的基因.对比br基因编码蛋白螺旋C到G的序列发现它们具有相似的种属特征.采用BR和HR蛋白螺旋C到G序列构建系统发生树比传统的16S rDNA序列具有优势，BR和HR可以作为两种新型的分子指标. |
| first_indexed | 2024-04-24T17:00:50Z |
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| id | doaj.art-aa26a0a93021416ea23be2dcc470f79a |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:00:50Z |
| publishDate | 2008-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-aa26a0a93021416ea23be2dcc470f79a2024-03-29T01:58:25ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972008-01-013518086zjup/1008-9497.2008.35.1.80-86Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析)SHIHan(时晗)0WUMin(吴敏)1XUXue-wei(许学伟)2ZHOUZhen(周祯)3College of Life Sciences, Zhejiang University, Hangzhou 310058, China(浙江大学生命科学学院，浙江 杭州 310058)College of Life Sciences, Zhejiang University, Hangzhou 310058, China(浙江大学生命科学学院，浙江 杭州 310058)College of Life Sciences, Zhejiang University, Hangzhou 310058, China(浙江大学生命科学学院，浙江 杭州 310058)College of Life Sciences, Zhejiang University, Hangzhou 310058, China(浙江大学生命科学学院，浙江 杭州 310058)采用PCR方法扩增了嗜盐古菌AB19、AB30和AJ5编码螺旋C至螺旋G的氯视紫红质(halorhodopsin，HR)蛋白基因片断和16S rRNA基因(16S rDNA)，测定了它们的核苷酸序列，与已报道的hr基因序列差异显著.获得AJ5 HR蛋白基因序列在Halobiforma属中尚属首次.对hr基因序列进行的遗传分析，包括GC含量、转换与颠换的比值、密码子使用偏好，表明hr基因面临进化选择和偏倚突变压的双重制约，是一类进化程度较高的基因.对比br基因编码蛋白螺旋C到G的序列发现它们具有相似的种属特征.采用BR和HR蛋白螺旋C到G序列构建系统发生树比传统的16S rDNA序列具有优势，BR和HR可以作为两种新型的分子指标.https://doi.org/zjup/1008-9497.2008.35.1.80-86嗜盐古菌氯视紫红质细菌视紫红质遗传分析16s rdna |
| spellingShingle | SHIHan(时晗) WUMin(吴敏) XUXue-wei(许学伟) ZHOUZhen(周祯) Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) Zhejiang Daxue xuebao. Lixue ban 嗜盐古菌 氯视紫红质 细菌视紫红质 遗传分析 16s rdna |
| title | Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) |
| title_full | Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) |
| title_fullStr | Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) |
| title_full_unstemmed | Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) |
| title_short | Genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea(嗜盐古菌中氯视紫红质基因序列的遗传分析) |
| title_sort | genetic analysis of partial sequences of the gene for halorhodopsin in halophilic archaea 嗜盐古菌中氯视紫红质基因序列的遗传分析 |
| topic | 嗜盐古菌 氯视紫红质 细菌视紫红质 遗传分析 16s rdna |
| url | https://doi.org/zjup/1008-9497.2008.35.1.80-86 |
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