Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection
Human rhinoviruses (HRVs) are the main cause of cold-like illnesses, and currently no vaccine or antiviral therapies against HRVs are available to prevent or mitigate HRV infection. There are more than 150 antigenically heterogeneous HRV serotypes, with ∼90 HRVs belonging to major group species A an...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01646/full |
| _version_ | 1811270629079384064 |
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| author | Mira C. Patel Lioubov M. Pletneva Marina S. Boukhvalova Stefanie N. Vogel Adriana E. Kajon Jorge C. G. Blanco |
| author_facet | Mira C. Patel Lioubov M. Pletneva Marina S. Boukhvalova Stefanie N. Vogel Adriana E. Kajon Jorge C. G. Blanco |
| author_sort | Mira C. Patel |
| collection | DOAJ |
| description | Human rhinoviruses (HRVs) are the main cause of cold-like illnesses, and currently no vaccine or antiviral therapies against HRVs are available to prevent or mitigate HRV infection. There are more than 150 antigenically heterogeneous HRV serotypes, with ∼90 HRVs belonging to major group species A and B. Development of small animal models that are susceptible to infection with major group HRVs would be beneficial for vaccine research. Previously, we showed that the cotton rat (Sigmodon hispidus) is semi-permissive to HRV16 (major group, species HRV-A virus) infection, replicating in the upper and lower respiratory tracts with measurable pathology, mucus production, and expression of inflammatory mediators. Herein, we report that intranasal infection of cotton rats with HRV14 (major group, species HRV-B virus) results in isolation of infectious virus from the nose and lung. Similar to HRV16, intramuscular immunization with live HRV14 induces homologous protection that correlated with high levels of serum neutralizing antibodies. Vaccination and challenge experiments with HRV14 and HRV16 to evaluate the development of cross-protective immunity demonstrate that intramuscular immunization with live HRV16 significantly protects animals against HRV14 challenge. Determination of the immunological mechanisms involved in heterologous protection and further characterization of infection with other major HRV serotypes in the cotton rat could enhance the robustness of the model to define heterotypic relationships between this diverse group of viruses and thereby increase its potential for development of a multi-serotype HRV vaccine. |
| first_indexed | 2024-04-12T22:05:39Z |
| format | Article |
| id | doaj.art-aa27149e3bbe4e658e74b6c82fbe2de5 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T22:05:39Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aa27149e3bbe4e658e74b6c82fbe2de52022-12-22T03:14:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01646286196Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 InfectionMira C. Patel0Lioubov M. Pletneva1Marina S. Boukhvalova2Stefanie N. Vogel3Adriana E. Kajon4Jorge C. G. Blanco5Sigmovir Biosystems, Inc., RockvilleMD, United StatesSigmovir Biosystems, Inc., RockvilleMD, United StatesSigmovir Biosystems, Inc., RockvilleMD, United StatesUniversity of Maryland School of Medicine, BaltimoreMD, United StatesInfectious Disease Program, Lovelace Respiratory Research Institute, AlbuquerqueNM, United StatesSigmovir Biosystems, Inc., RockvilleMD, United StatesHuman rhinoviruses (HRVs) are the main cause of cold-like illnesses, and currently no vaccine or antiviral therapies against HRVs are available to prevent or mitigate HRV infection. There are more than 150 antigenically heterogeneous HRV serotypes, with ∼90 HRVs belonging to major group species A and B. Development of small animal models that are susceptible to infection with major group HRVs would be beneficial for vaccine research. Previously, we showed that the cotton rat (Sigmodon hispidus) is semi-permissive to HRV16 (major group, species HRV-A virus) infection, replicating in the upper and lower respiratory tracts with measurable pathology, mucus production, and expression of inflammatory mediators. Herein, we report that intranasal infection of cotton rats with HRV14 (major group, species HRV-B virus) results in isolation of infectious virus from the nose and lung. Similar to HRV16, intramuscular immunization with live HRV14 induces homologous protection that correlated with high levels of serum neutralizing antibodies. Vaccination and challenge experiments with HRV14 and HRV16 to evaluate the development of cross-protective immunity demonstrate that intramuscular immunization with live HRV16 significantly protects animals against HRV14 challenge. Determination of the immunological mechanisms involved in heterologous protection and further characterization of infection with other major HRV serotypes in the cotton rat could enhance the robustness of the model to define heterotypic relationships between this diverse group of viruses and thereby increase its potential for development of a multi-serotype HRV vaccine.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01646/fullrhinovirusescross-protectionvaccinescotton ratasthma |
| spellingShingle | Mira C. Patel Lioubov M. Pletneva Marina S. Boukhvalova Stefanie N. Vogel Adriana E. Kajon Jorge C. G. Blanco Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection Frontiers in Microbiology rhinoviruses cross-protection vaccines cotton rat asthma |
| title | Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection |
| title_full | Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection |
| title_fullStr | Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection |
| title_full_unstemmed | Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection |
| title_short | Immunization with Live Human Rhinovirus (HRV) 16 Induces Protection in Cotton Rats against HRV14 Infection |
| title_sort | immunization with live human rhinovirus hrv 16 induces protection in cotton rats against hrv14 infection |
| topic | rhinoviruses cross-protection vaccines cotton rat asthma |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01646/full |
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