A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome
Summary: This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302148 |
| _version_ | 1818337291469324288 |
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| author | Laura Payling Nicole C. Roy Karl Fraser Simon M. Loveday Ian M. Sims Peter H. Janssen Stefan J. Hill Laura G. Raymond Warren C. McNabb |
| author_facet | Laura Payling Nicole C. Roy Karl Fraser Simon M. Loveday Ian M. Sims Peter H. Janssen Stefan J. Hill Laura G. Raymond Warren C. McNabb |
| author_sort | Laura Payling |
| collection | DOAJ |
| description | Summary: This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions. |
| first_indexed | 2024-12-13T14:52:53Z |
| format | Article |
| id | doaj.art-aa299fb328a14b469352a0d8781210b5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T14:52:53Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-aa299fb328a14b469352a0d8781210b52022-12-21T23:41:18ZengElsevierSTAR Protocols2666-16672021-03-0121100227A protocol combining breath testing and ex vivo fermentations to study the human gut microbiomeLaura Payling0Nicole C. Roy1Karl Fraser2Simon M. Loveday3Ian M. Sims4Peter H. Janssen5Stefan J. Hill6Laura G. Raymond7Warren C. McNabb8School of Food and Advanced Technology, Massey University, Palmerston North 4410, New Zealand; Riddet Institute, Massey University, Palmerston North 4410, New Zealand; Food and Bio-Based Products, AgResearch Limited, Tennent Drive, Palmerston North 4410, New Zealand; Corresponding authorRiddet Institute, Massey University, Palmerston North 4410, New Zealand; Department of Nutrition, University of Otago, Dunedin 9016, New Zealand; Liggins Institute, The University of Auckland, Auckland 1023, New Zealand; High-Value Nutrition National Science Challenge, Auckland 1023, New ZealandRiddet Institute, Massey University, Palmerston North 4410, New Zealand; Food and Bio-Based Products, AgResearch Limited, Tennent Drive, Palmerston North 4410, New Zealand; High-Value Nutrition National Science Challenge, Auckland 1023, New ZealandRiddet Institute, Massey University, Palmerston North 4410, New Zealand; Food and Bio-Based Products, AgResearch Limited, Tennent Drive, Palmerston North 4410, New ZealandFerrier Research Institute, Victoria University of Wellington, Wellington 5010, New ZealandFood and Bio-Based Products, AgResearch Limited, Tennent Drive, Palmerston North 4410, New ZealandAdvanced Chemical Characterisation, Scion, Te Papa Tipu Innovation Park, Rotorua 3010, New ZealandAdvanced Chemical Characterisation, Scion, Te Papa Tipu Innovation Park, Rotorua 3010, New ZealandRiddet Institute, Massey University, Palmerston North 4410, New Zealand; High-Value Nutrition National Science Challenge, Auckland 1023, New ZealandSummary: This protocol describes the application of breath testing and ex vivo fermentations to study the association between breath methane and the composition and functionality of the gut microbiome. The protocol provides a useful systems biology approach for studying the gut microbiome in humans, which combines standardized methods in human breath testing and fecal sampling. The model described is accessible and easy to repeat, but its relative simplicity means that it can deviate from human physiological conditions.http://www.sciencedirect.com/science/article/pii/S2666166720302148Cell CultureClinical ProtocolGenomics |
| spellingShingle | Laura Payling Nicole C. Roy Karl Fraser Simon M. Loveday Ian M. Sims Peter H. Janssen Stefan J. Hill Laura G. Raymond Warren C. McNabb A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome STAR Protocols Cell Culture Clinical Protocol Genomics |
| title | A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| title_full | A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| title_fullStr | A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| title_full_unstemmed | A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| title_short | A protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| title_sort | protocol combining breath testing and ex vivo fermentations to study the human gut microbiome |
| topic | Cell Culture Clinical Protocol Genomics |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720302148 |
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