Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS
Abstract Background The in vitro maturation (IVM) technique has physical and financial benefits, but a lower efficiency and outcome that is still unclear whether it is related to polycystic ovary syndrome (PCOS) itself or the IVM procedure. In this study, we analyzed the clinical and laboratory outc...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12958-020-00622-y |
| _version_ | 1828323732799619072 |
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| author | Nagwa Elshewy Dongmei Ji Zhiguo Zhang Dawei Chen Beili Chen Rufeng Xue Huan Wu Jianye Wang Ping Zhou Yunxia Cao |
| author_facet | Nagwa Elshewy Dongmei Ji Zhiguo Zhang Dawei Chen Beili Chen Rufeng Xue Huan Wu Jianye Wang Ping Zhou Yunxia Cao |
| author_sort | Nagwa Elshewy |
| collection | DOAJ |
| description | Abstract Background The in vitro maturation (IVM) technique has physical and financial benefits, but a lower efficiency and outcome that is still unclear whether it is related to polycystic ovary syndrome (PCOS) itself or the IVM procedure. In this study, we analyzed the clinical and laboratory outcomes of an optimized IVM protocol in patients with and without PCOS. We also discussed the possible reasons for early embryo arrest in the IVM cycle. Methods This prospective study involved 58 PCOS patients and 56 non-PCOS patients who underwent mild stimulated IVF combined IVM (IVF/M) cycles. The clinical and laboratory outcomes were compared between the two groups. Also, metaphase II (MII) oocytes were obtained after IVM from the two groups, and in vivo MII oocytes randomly collected from IVF patients were examined for mitochondrial function using a laser scanning confocal microscope (LSCM). The aneuploidy rate for arrested cleavage embryos from IVM and IVF oocytes were screened using Next Generation Sequencing (NGS). Results Mildly stimulated IVF/M resulted in cumulative clinical pregnancy and implantation rates (40.2, 28.7% in the PCOS group vs. 41.9, 36% in the non-PCOS group), respectively. The blastocyst formation rates were comparable (28% vs. 28.2%) in PCOS and non-PCOS groups, respectively. Using LSCM, there was a significant decrease in the mitochondrial membrane potential of IVM oocytes compared with the control IVF oocytes (P < 0.001), but no significant difference between the PCOS and non-PCOS groups. The NGS showed that the aneuploidy rates were comparable (75, 75, and 66.6%) in IVM-PCOS, IVM-non-PCOS, and control IVF arrested embryos, respectively. Conclusions The mildly stimulated IVF/M protocol produced acceptable clinical outcomes in PCOS and non-PCOS patients. IVM itself rather than the PCOS condition adversely affected the embryo development through its effect on mitochondrial function, which appeared to be a possible cause for the embryo arrest in the IVM cycles rather than chromosomal aneuploidy. |
| first_indexed | 2024-04-13T18:56:36Z |
| format | Article |
| id | doaj.art-aa2e541639f948768a4bc8b4707fcccf |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-13T18:56:36Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-aa2e541639f948768a4bc8b4707fcccf2022-12-22T02:34:14ZengBMCReproductive Biology and Endocrinology1477-78272020-07-0118111110.1186/s12958-020-00622-yAssociation between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOSNagwa Elshewy0Dongmei Ji1Zhiguo Zhang2Dawei Chen3Beili Chen4Rufeng Xue5Huan Wu6Jianye Wang7Ping Zhou8Yunxia Cao9Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityReproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical UniversityAbstract Background The in vitro maturation (IVM) technique has physical and financial benefits, but a lower efficiency and outcome that is still unclear whether it is related to polycystic ovary syndrome (PCOS) itself or the IVM procedure. In this study, we analyzed the clinical and laboratory outcomes of an optimized IVM protocol in patients with and without PCOS. We also discussed the possible reasons for early embryo arrest in the IVM cycle. Methods This prospective study involved 58 PCOS patients and 56 non-PCOS patients who underwent mild stimulated IVF combined IVM (IVF/M) cycles. The clinical and laboratory outcomes were compared between the two groups. Also, metaphase II (MII) oocytes were obtained after IVM from the two groups, and in vivo MII oocytes randomly collected from IVF patients were examined for mitochondrial function using a laser scanning confocal microscope (LSCM). The aneuploidy rate for arrested cleavage embryos from IVM and IVF oocytes were screened using Next Generation Sequencing (NGS). Results Mildly stimulated IVF/M resulted in cumulative clinical pregnancy and implantation rates (40.2, 28.7% in the PCOS group vs. 41.9, 36% in the non-PCOS group), respectively. The blastocyst formation rates were comparable (28% vs. 28.2%) in PCOS and non-PCOS groups, respectively. Using LSCM, there was a significant decrease in the mitochondrial membrane potential of IVM oocytes compared with the control IVF oocytes (P < 0.001), but no significant difference between the PCOS and non-PCOS groups. The NGS showed that the aneuploidy rates were comparable (75, 75, and 66.6%) in IVM-PCOS, IVM-non-PCOS, and control IVF arrested embryos, respectively. Conclusions The mildly stimulated IVF/M protocol produced acceptable clinical outcomes in PCOS and non-PCOS patients. IVM itself rather than the PCOS condition adversely affected the embryo development through its effect on mitochondrial function, which appeared to be a possible cause for the embryo arrest in the IVM cycles rather than chromosomal aneuploidy.http://link.springer.com/article/10.1186/s12958-020-00622-yIVMEmbryo arrestMitochondriaAneuploidy |
| spellingShingle | Nagwa Elshewy Dongmei Ji Zhiguo Zhang Dawei Chen Beili Chen Rufeng Xue Huan Wu Jianye Wang Ping Zhou Yunxia Cao Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS Reproductive Biology and Endocrinology IVM Embryo arrest Mitochondria Aneuploidy |
| title | Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS |
| title_full | Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS |
| title_fullStr | Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS |
| title_full_unstemmed | Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS |
| title_short | Association between mild stimulated IVF/M cycle and early embryo arrest in sub fertile women with/without PCOS |
| title_sort | association between mild stimulated ivf m cycle and early embryo arrest in sub fertile women with without pcos |
| topic | IVM Embryo arrest Mitochondria Aneuploidy |
| url | http://link.springer.com/article/10.1186/s12958-020-00622-y |
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