Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers
The genetic diversity among 78 cucumber (Cucumis sativus L.) accessions was analyzed using 8 morphological traits and 60 SSR markers under two environmental (open field and protected cultivation) conditions. D2 analysis had grouped the 78 accessions in five major clusters. Cluster I comprised 51 acc...
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Indian Council of Agricultural Research
2020-12-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/108583 |
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| author | RAHUL KUMAR A D MUNSHI T K BEHERA G S JAT H CHOUDHARY MAMTA SINGH AKSHAY TALUKDAR |
| author_facet | RAHUL KUMAR A D MUNSHI T K BEHERA G S JAT H CHOUDHARY MAMTA SINGH AKSHAY TALUKDAR |
| author_sort | RAHUL KUMAR |
| collection | DOAJ |
| description | The genetic diversity among 78 cucumber (Cucumis sativus L.) accessions was analyzed using 8 morphological traits and 60 SSR markers under two environmental (open field and protected cultivation) conditions. D2 analysis had grouped the 78 accessions in five major clusters. Cluster I comprised 51 accessions followed by 14, 5, 7 and 1 in cluster II, III, IV, and V respectively. The variation was observed for morphological characters like days to first female flower anthesis (37.53-58.64), days to first fruit harvest (47.28-67.43), fruit length (9.47-26.84 cm), average fruit weight (67.46-417.56 g) and vine length (96.23-170.13 cm). The first four principal components explained 87.72% of the total variation. A total of 171 alleles were amplified with a mean of 2.85 alleles per locus. The polymorphism information content (PIC) varied from 0.05 (UW084478) to 0.59 (UW084186) with a mean value of 0.36. The major allele frequency, gene diversity, and heterozygosity of these SSR markers were 0.36-0.97, 0.05-0.67 and 0.00-0.68, respectively. The dendrogram based on SSR marker analysis classified the 78 genotypes into two major groups those were subdivided into ten subgroups. Collectively, the information obtained will provide a valuable resource for germplasm conservation, genetic analyses and gene discovery in cucumber breeding. |
| first_indexed | 2024-04-10T15:27:13Z |
| format | Article |
| id | doaj.art-aa3050fe3eda43bc8bc8f2d7aad4fa69 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T15:27:13Z |
| publishDate | 2020-12-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-aa3050fe3eda43bc8bc8f2d7aad4fa692023-02-14T08:13:08ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192020-12-01901110.56093/ijas.v90i11.108583Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markersRAHUL KUMAR0A D MUNSHI1T K BEHERA2G S JAT3H CHOUDHARY4MAMTA SINGH5AKSHAY TALUKDAR6Ph D Scholar, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, IndiaPrincipal Scientist, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, IndiaProfessor and Principal Scientist, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, IndiaScientist, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, IndiaPrincipal Scientist, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi 110 012, IndiaScientist, Division of Germplasm Conservation, ICARNational Bureau of Plant Genetic Resources, New Delhi 110012, IndiaPrincipal Scientist, Division of Genetics, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi, India 110012The genetic diversity among 78 cucumber (Cucumis sativus L.) accessions was analyzed using 8 morphological traits and 60 SSR markers under two environmental (open field and protected cultivation) conditions. D2 analysis had grouped the 78 accessions in five major clusters. Cluster I comprised 51 accessions followed by 14, 5, 7 and 1 in cluster II, III, IV, and V respectively. The variation was observed for morphological characters like days to first female flower anthesis (37.53-58.64), days to first fruit harvest (47.28-67.43), fruit length (9.47-26.84 cm), average fruit weight (67.46-417.56 g) and vine length (96.23-170.13 cm). The first four principal components explained 87.72% of the total variation. A total of 171 alleles were amplified with a mean of 2.85 alleles per locus. The polymorphism information content (PIC) varied from 0.05 (UW084478) to 0.59 (UW084186) with a mean value of 0.36. The major allele frequency, gene diversity, and heterozygosity of these SSR markers were 0.36-0.97, 0.05-0.67 and 0.00-0.68, respectively. The dendrogram based on SSR marker analysis classified the 78 genotypes into two major groups those were subdivided into ten subgroups. Collectively, the information obtained will provide a valuable resource for germplasm conservation, genetic analyses and gene discovery in cucumber breeding.https://epubs.icar.org.in/index.php/IJAgS/article/view/108583Cucumis sativusGenetic diversityMorphologicalSimple sequence repeat (SSR) Morphological markers |
| spellingShingle | RAHUL KUMAR A D MUNSHI T K BEHERA G S JAT H CHOUDHARY MAMTA SINGH AKSHAY TALUKDAR Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers The Indian Journal of Agricultural Sciences Cucumis sativus Genetic diversity Morphological Simple sequence repeat (SSR) Morphological markers |
| title | Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers |
| title_full | Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers |
| title_fullStr | Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers |
| title_full_unstemmed | Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers |
| title_short | Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers |
| title_sort | genetic diversity of cucumber cucumis sativus accessions differing in quantitative traits and microsatellite markers |
| topic | Cucumis sativus Genetic diversity Morphological Simple sequence repeat (SSR) Morphological markers |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/108583 |
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