Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study)
Isolation and identificationof the bacteria-infected hydatid cystsof livestock animals were carried out. The study lasted for five months. A total of 302 slaughtered livestock were checked to find hydatid cyst naturally infected livers and lungs of sheep and goat at local Mosul abattoir/ Nineveh/ Ir...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2022-06-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_173309_8634e11a251e8ca8e8e46a916701145b.pdf |
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| author | Maimonah Yahya Zahraa Mohi-Aldeen |
| author_facet | Maimonah Yahya Zahraa Mohi-Aldeen |
| author_sort | Maimonah Yahya |
| collection | DOAJ |
| description | Isolation and identificationof the bacteria-infected hydatid cystsof livestock animals were carried out. The study lasted for five months. A total of 302 slaughtered livestock were checked to find hydatid cyst naturally infected livers and lungs of sheep and goat at local Mosul abattoir/ Nineveh/ Iraq. Each cysts' aspirated fluid was cultured separately on blood or nutrient agar at 37C for 24 hours then detected secondary bacteria. Each bacterium was incubated independently with 1000 viable protoscolices in a culture tube containing tryptic soy broth at 37°C and examined every two hours for 6 hours, followed by 12, 24, 36, and 48 hours afterward.The degeneration rate of protoscolices resulting from treatment with bacteria isolated depending on viability was studied <em>in vitro</em>.The results were revealed that the liver was the preponderant affected organ in sheep and goats. About 57.1% of sheep and 50% of goats harbored fertile cysts. <em>Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Proteus mirabilis</em>, and <em>Pseudomonas aeruginosa</em> bacteria were observed in the infected cysts. The most common bacteria infect the cyst was <em>Escherichia coli</em>. The protoscoleces treated with the bacterial isolates had completely deteriorated, whereas 97% of protoscoleces throughout the control groups were still alive and intact even after completing the incubation time. The experiments detect a significant time-dependent scolicidal effect on decreased viability of protoscolices <em>in vitro</em> study. This could pave the door for more research into the scolicidal validity of bacteria or their by-products as protoscolices both <em>in vitro </em>and<em> in vivo</em>. |
| first_indexed | 2024-12-12T08:32:39Z |
| format | Article |
| id | doaj.art-aa3645f4a00246dbbb1d0a62590cf14e |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-12T08:32:39Z |
| publishDate | 2022-06-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-aa3645f4a00246dbbb1d0a62590cf14e2022-12-22T00:31:03ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552022-06-0136376176810.33899/ijvs.2022.131873.2016173309Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study)Maimonah Yahya0Zahraa Mohi-Aldeen1Department Clinical Laboratory Science, College of pharmacy, University of Mosul, Mosul - Iraq.Biology Department, College of Science, University of Mosul, Mosul, Iraq.Isolation and identificationof the bacteria-infected hydatid cystsof livestock animals were carried out. The study lasted for five months. A total of 302 slaughtered livestock were checked to find hydatid cyst naturally infected livers and lungs of sheep and goat at local Mosul abattoir/ Nineveh/ Iraq. Each cysts' aspirated fluid was cultured separately on blood or nutrient agar at 37C for 24 hours then detected secondary bacteria. Each bacterium was incubated independently with 1000 viable protoscolices in a culture tube containing tryptic soy broth at 37°C and examined every two hours for 6 hours, followed by 12, 24, 36, and 48 hours afterward.The degeneration rate of protoscolices resulting from treatment with bacteria isolated depending on viability was studied <em>in vitro</em>.The results were revealed that the liver was the preponderant affected organ in sheep and goats. About 57.1% of sheep and 50% of goats harbored fertile cysts. <em>Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Proteus mirabilis</em>, and <em>Pseudomonas aeruginosa</em> bacteria were observed in the infected cysts. The most common bacteria infect the cyst was <em>Escherichia coli</em>. The protoscoleces treated with the bacterial isolates had completely deteriorated, whereas 97% of protoscoleces throughout the control groups were still alive and intact even after completing the incubation time. The experiments detect a significant time-dependent scolicidal effect on decreased viability of protoscolices <em>in vitro</em> study. This could pave the door for more research into the scolicidal validity of bacteria or their by-products as protoscolices both <em>in vitro </em>and<em> in vivo</em>.https://vetmedmosul.com/article_173309_8634e11a251e8ca8e8e46a916701145b.pdfe. granulosusprotoscolicesviability |
| spellingShingle | Maimonah Yahya Zahraa Mohi-Aldeen Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) Iraqi Journal of Veterinary Sciences e. granulosus protoscolices viability |
| title | Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) |
| title_full | Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) |
| title_fullStr | Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) |
| title_full_unstemmed | Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) |
| title_short | Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study) |
| title_sort | secondary bacterial infection of hydatid cysts infected livestock animals in vitro study |
| topic | e. granulosus protoscolices viability |
| url | https://vetmedmosul.com/article_173309_8634e11a251e8ca8e8e46a916701145b.pdf |
| work_keys_str_mv | AT maimonahyahya secondarybacterialinfectionofhydatidcystsinfectedlivestockanimalsinvitrostudy AT zahraamohialdeen secondarybacterialinfectionofhydatidcystsinfectedlivestockanimalsinvitrostudy |