Dynamics of maternal gene expression in Rhodnius prolixus

Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish th...

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Main Authors: Agustina Pascual, Rolando Rivera-Pomar
Format: Article
Language:English
Published: Nature Portfolio 2022-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-09874-7
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author Agustina Pascual
Rolando Rivera-Pomar
author_facet Agustina Pascual
Rolando Rivera-Pomar
author_sort Agustina Pascual
collection DOAJ
description Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D (Rp-BicD) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus, we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus.
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spelling doaj.art-aa364ae9d30a4f57bd315563eb4109b42022-12-22T02:22:08ZengNature PortfolioScientific Reports2045-23222022-04-0112111710.1038/s41598-022-09874-7Dynamics of maternal gene expression in Rhodnius prolixusAgustina Pascual0Rolando Rivera-Pomar1Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D (Rp-BicD) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus, we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus.https://doi.org/10.1038/s41598-022-09874-7
spellingShingle Agustina Pascual
Rolando Rivera-Pomar
Dynamics of maternal gene expression in Rhodnius prolixus
Scientific Reports
title Dynamics of maternal gene expression in Rhodnius prolixus
title_full Dynamics of maternal gene expression in Rhodnius prolixus
title_fullStr Dynamics of maternal gene expression in Rhodnius prolixus
title_full_unstemmed Dynamics of maternal gene expression in Rhodnius prolixus
title_short Dynamics of maternal gene expression in Rhodnius prolixus
title_sort dynamics of maternal gene expression in rhodnius prolixus
url https://doi.org/10.1038/s41598-022-09874-7
work_keys_str_mv AT agustinapascual dynamicsofmaternalgeneexpressioninrhodniusprolixus
AT rolandoriverapomar dynamicsofmaternalgeneexpressioninrhodniusprolixus