Dynamics of maternal gene expression in Rhodnius prolixus
Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish th...
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Nature Portfolio
2022-04-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-022-09874-7 |
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author | Agustina Pascual Rolando Rivera-Pomar |
author_facet | Agustina Pascual Rolando Rivera-Pomar |
author_sort | Agustina Pascual |
collection | DOAJ |
description | Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D (Rp-BicD) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus, we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus. |
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id | doaj.art-aa364ae9d30a4f57bd315563eb4109b4 |
institution | Directory Open Access Journal |
issn | 2045-2322 |
language | English |
last_indexed | 2024-04-14T00:42:40Z |
publishDate | 2022-04-01 |
publisher | Nature Portfolio |
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spelling | doaj.art-aa364ae9d30a4f57bd315563eb4109b42022-12-22T02:22:08ZengNature PortfolioScientific Reports2045-23222022-04-0112111710.1038/s41598-022-09874-7Dynamics of maternal gene expression in Rhodnius prolixusAgustina Pascual0Rolando Rivera-Pomar1Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)Centro de BioInvestigaciones (CeBio-CICBA), Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA)Abstract The study of developmental processes in Rhodnius prolixus has recently advanced with the sequencing of the genome. In this work, we analyze the maternal gene expression driving oogenesis and early embryogenesis in R. prolixus. We examined the transcriptional profile of mRNAs to establish the genes expressed across the ovary, unfertilized eggs and different embryonic stages of R. prolixus until the formation of the germ band anlage (0, 12, 24, and 48 h post egg laying). We identified 81 putative maternal and ovary-related genes and validated their expression by qRT-PCR. We validate the function of the ortholog gene Bicaudal-D (Rp-BicD) by in situ hybridization and parental RNAi. Consistent with a role in oogenesis and early development of R. prolixus, we show that lack of Rp-BicD does not significantly affect oogenesis but impairs the formation of the blastoderm. Based on our findings, we propose three times of action for maternal genes during oogenesis and embryogenesis in R. prolixus.https://doi.org/10.1038/s41598-022-09874-7 |
spellingShingle | Agustina Pascual Rolando Rivera-Pomar Dynamics of maternal gene expression in Rhodnius prolixus Scientific Reports |
title | Dynamics of maternal gene expression in Rhodnius prolixus |
title_full | Dynamics of maternal gene expression in Rhodnius prolixus |
title_fullStr | Dynamics of maternal gene expression in Rhodnius prolixus |
title_full_unstemmed | Dynamics of maternal gene expression in Rhodnius prolixus |
title_short | Dynamics of maternal gene expression in Rhodnius prolixus |
title_sort | dynamics of maternal gene expression in rhodnius prolixus |
url | https://doi.org/10.1038/s41598-022-09874-7 |
work_keys_str_mv | AT agustinapascual dynamicsofmaternalgeneexpressioninrhodniusprolixus AT rolandoriverapomar dynamicsofmaternalgeneexpressioninrhodniusprolixus |