Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging
Summary: Confocal, multiphoton, or other advanced microscopy techniques produce high-quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protoco...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000137 |
| _version_ | 1819163632304390144 |
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| author | Brent Asrican Juan Song |
| author_facet | Brent Asrican Juan Song |
| author_sort | Brent Asrican |
| collection | DOAJ |
| description | Summary: Confocal, multiphoton, or other advanced microscopy techniques produce high-quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protocol to extract meaningful features of both somatic neuronal and membranous astrocytic calcium dynamics obtained from charge-coupled device (CCD)-based camera setups, typical of electrophysiology rigs and highly relevant for investigating neuronal and astrocytic involvement in brain circuitry.For complete details on the use and execution of this protocol, please refer to Asrican et al. (2020). |
| first_indexed | 2024-12-22T17:47:13Z |
| format | Article |
| id | doaj.art-aa3710d2d07d4540bd1541e26415a78f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-22T17:47:13Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-aa3710d2d07d4540bd1541e26415a78f2022-12-21T18:18:15ZengElsevierSTAR Protocols2666-16672021-03-0121100306Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imagingBrent Asrican0Juan Song1Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Corresponding authorDepartment of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Corresponding authorSummary: Confocal, multiphoton, or other advanced microscopy techniques produce high-quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protocol to extract meaningful features of both somatic neuronal and membranous astrocytic calcium dynamics obtained from charge-coupled device (CCD)-based camera setups, typical of electrophysiology rigs and highly relevant for investigating neuronal and astrocytic involvement in brain circuitry.For complete details on the use and execution of this protocol, please refer to Asrican et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000137Cell biologyMicroscopyNeuroscience |
| spellingShingle | Brent Asrican Juan Song Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging STAR Protocols Cell biology Microscopy Neuroscience |
| title | Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging |
| title_full | Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging |
| title_fullStr | Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging |
| title_full_unstemmed | Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging |
| title_short | Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging |
| title_sort | extracting meaningful circuit based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single photon gcamp imaging |
| topic | Cell biology Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721000137 |
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