Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance
Abstract Background Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces and Guanxi autonomous region of China. However, trees growing in these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolom...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04983-z |
| _version_ | 1827284411999059968 |
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| author | Yu Kong Xianbin Hou Zhenglu Liu Yufeng Li |
| author_facet | Yu Kong Xianbin Hou Zhenglu Liu Yufeng Li |
| author_sort | Yu Kong |
| collection | DOAJ |
| description | Abstract Background Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces and Guanxi autonomous region of China. However, trees growing in these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome and transcriptome profiles of leaves are limited. Here, we used combined metabolome and transcriptome analyses of leaves of three mango cultivars with different cold stress tolerance, i.e. Jinhuang (J)—tolerant, Tainung (T) and Guiremang No. 82 (G)—susceptible, after 24 (LF), 48 (MF) and 72 (HF) hours of cold. Results A total of 1,323 metabolites belonging to 12 compound classes were detected. Of these, amino acids and derivatives, nucleotides and derivatives, and lipids accumulated in higher quantities after cold stress exposure in the three cultivars. Notably, Jinhuang leaves showed increasing accumulation trends of flavonoids, terpenoids, lignans and coumarins, and alkaloids with exposure time. Among the phytohormones, jasmonic acid and abscisic acid levels decreased, while N6-isopentenyladenine increased with cold stress time. Transcriptome analysis led to the identification of 22,526 differentially expressed genes. Many genes enriched in photosynthesis, antenna proteins, flavonoid, terpenoid (di- and sesquiterpenoids) and alkaloid biosynthesis pathways were upregulated in Jihuang leaves. Moreover, expression changes related to phytohormones, MAPK (including calcium and H2O2), and the ICE-CBF-COR signalling cascade indicate involvement of these pathways in cold stress responses. Conclusion Cold stress tolerance in mango leaves is associated with regulation of primary and secondary metabolite biosynthesis pathways. Jasmonic acid, abscisic acid, and cytokinins are potential regulators of cold stress responses in mango leaves. |
| first_indexed | 2024-04-24T09:54:35Z |
| format | Article |
| id | doaj.art-aa374bb1d42944aca4c88855eb612ae3 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-24T09:54:35Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-aa374bb1d42944aca4c88855eb612ae32024-04-14T11:11:57ZengBMCBMC Plant Biology1471-22292024-04-0124111710.1186/s12870-024-04983-zCold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold toleranceYu Kong0Xianbin Hou1Zhenglu Liu2Yufeng Li3Guangxi Key Laboratory of Biology for Mongo, Baise UniversityGuangxi Key Laboratory of Biology for Mongo, Baise UniversityGuangxi Key Laboratory of Biology for Mongo, Baise UniversityGuangxi Key Laboratory of Biology for Mongo, Baise UniversityAbstract Background Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces and Guanxi autonomous region of China. However, trees growing in these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome and transcriptome profiles of leaves are limited. Here, we used combined metabolome and transcriptome analyses of leaves of three mango cultivars with different cold stress tolerance, i.e. Jinhuang (J)—tolerant, Tainung (T) and Guiremang No. 82 (G)—susceptible, after 24 (LF), 48 (MF) and 72 (HF) hours of cold. Results A total of 1,323 metabolites belonging to 12 compound classes were detected. Of these, amino acids and derivatives, nucleotides and derivatives, and lipids accumulated in higher quantities after cold stress exposure in the three cultivars. Notably, Jinhuang leaves showed increasing accumulation trends of flavonoids, terpenoids, lignans and coumarins, and alkaloids with exposure time. Among the phytohormones, jasmonic acid and abscisic acid levels decreased, while N6-isopentenyladenine increased with cold stress time. Transcriptome analysis led to the identification of 22,526 differentially expressed genes. Many genes enriched in photosynthesis, antenna proteins, flavonoid, terpenoid (di- and sesquiterpenoids) and alkaloid biosynthesis pathways were upregulated in Jihuang leaves. Moreover, expression changes related to phytohormones, MAPK (including calcium and H2O2), and the ICE-CBF-COR signalling cascade indicate involvement of these pathways in cold stress responses. Conclusion Cold stress tolerance in mango leaves is associated with regulation of primary and secondary metabolite biosynthesis pathways. Jasmonic acid, abscisic acid, and cytokinins are potential regulators of cold stress responses in mango leaves.https://doi.org/10.1186/s12870-024-04983-zAmino acids and derivativesChilling stressFlavonoid biosynthesisICE-CBF-CORPhytohormone signalingTerpenoid biosynthesis |
| spellingShingle | Yu Kong Xianbin Hou Zhenglu Liu Yufeng Li Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance BMC Plant Biology Amino acids and derivatives Chilling stress Flavonoid biosynthesis ICE-CBF-COR Phytohormone signaling Terpenoid biosynthesis |
| title | Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| title_full | Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| title_fullStr | Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| title_full_unstemmed | Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| title_short | Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| title_sort | cold stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance |
| topic | Amino acids and derivatives Chilling stress Flavonoid biosynthesis ICE-CBF-COR Phytohormone signaling Terpenoid biosynthesis |
| url | https://doi.org/10.1186/s12870-024-04983-z |
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