Development of a clinical microarray system for genetic analysis screening
Objectives: Research on the relationship between diseases and genes and the advancement of genetic analysis technologies have made genetic testing in medical care possible. There are various methods for genetic testing, including PCR-based methods and next-generation sequencing; however, screening t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Practical Laboratory Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551722000452 |
| _version_ | 1797950683361574912 |
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| author | Hiroshi Okamura Hirofumi Yamano Toshiya Tsuda Junichi Morihiro Koichi Hirayama Hiroaki Nagano |
| author_facet | Hiroshi Okamura Hirofumi Yamano Toshiya Tsuda Junichi Morihiro Koichi Hirayama Hiroaki Nagano |
| author_sort | Hiroshi Okamura |
| collection | DOAJ |
| description | Objectives: Research on the relationship between diseases and genes and the advancement of genetic analysis technologies have made genetic testing in medical care possible. There are various methods for genetic testing, including PCR-based methods and next-generation sequencing; however, screening tests in clinical laboratories are becoming more diverse; therefore, novel measurement systems and equipment are required to meet the needs of each situation. In this study, we aimed to develop a novel microarray-based genetic analysis system that uses a Peltier element to overcome the issues of conventional microarrays, such as the long measurement time and high cost. Methods: We constructed a microarray system to detect the UDP-glucuronosyltransferase gene polymorphisms UGT1A1*6 and UGT1A1*28 in patients eligible for irinotecan hydrochloride treatment for use in clinical laboratories. To evaluate the performance of the system, the hybridization temperature and reaction time were determined, and the results were compared with those obtained using a conventional hybridization oven. Results: The hybridization temperature reached its target in 1/27th of the time required by the conventional system. We assessed 111 human clinical samples and found that our results agreed with those obtained using existing methods. The total time for the newly developed device was reduced by 85 min compared to that for existing methods, as the automated DNA microarray eliminates the time that existing methods spend on manual operation. Conclusions: The surface treatment technology used in our system enables high-density and strong DNA fixation, allowing the construction of a measurement system suitable for clinical applications. |
| first_indexed | 2024-04-10T22:20:03Z |
| format | Article |
| id | doaj.art-aa3764bdfbe742d59058a0f353140424 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-04-10T22:20:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-aa3764bdfbe742d59058a0f3531404242023-01-18T04:32:00ZengElsevierPractical Laboratory Medicine2352-55172023-01-0133e00306Development of a clinical microarray system for genetic analysis screeningHiroshi Okamura0Hirofumi Yamano1Toshiya Tsuda2Junichi Morihiro3Koichi Hirayama4Hiroaki Nagano5Toyo Kohan Co., Ltd., Shinagawa, Tokyo, Japan; Corresponding author. Toyo Kohan Co., Ltd., Japan.Toyo Kohan Co., Ltd., Shinagawa, Tokyo, JapanToyo Kohan Co., Ltd., Shinagawa, Tokyo, JapanToyo Kohan Co., Ltd., Shinagawa, Tokyo, JapanToyo Kohan Co., Ltd., Shinagawa, Tokyo, JapanDepartment of Gastroenterological, Breast and Endocrine Surgery, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, JapanObjectives: Research on the relationship between diseases and genes and the advancement of genetic analysis technologies have made genetic testing in medical care possible. There are various methods for genetic testing, including PCR-based methods and next-generation sequencing; however, screening tests in clinical laboratories are becoming more diverse; therefore, novel measurement systems and equipment are required to meet the needs of each situation. In this study, we aimed to develop a novel microarray-based genetic analysis system that uses a Peltier element to overcome the issues of conventional microarrays, such as the long measurement time and high cost. Methods: We constructed a microarray system to detect the UDP-glucuronosyltransferase gene polymorphisms UGT1A1*6 and UGT1A1*28 in patients eligible for irinotecan hydrochloride treatment for use in clinical laboratories. To evaluate the performance of the system, the hybridization temperature and reaction time were determined, and the results were compared with those obtained using a conventional hybridization oven. Results: The hybridization temperature reached its target in 1/27th of the time required by the conventional system. We assessed 111 human clinical samples and found that our results agreed with those obtained using existing methods. The total time for the newly developed device was reduced by 85 min compared to that for existing methods, as the automated DNA microarray eliminates the time that existing methods spend on manual operation. Conclusions: The surface treatment technology used in our system enables high-density and strong DNA fixation, allowing the construction of a measurement system suitable for clinical applications.http://www.sciencedirect.com/science/article/pii/S2352551722000452Genetic analysisMicroarrayMedical careScreening testSurface treatment |
| spellingShingle | Hiroshi Okamura Hirofumi Yamano Toshiya Tsuda Junichi Morihiro Koichi Hirayama Hiroaki Nagano Development of a clinical microarray system for genetic analysis screening Practical Laboratory Medicine Genetic analysis Microarray Medical care Screening test Surface treatment |
| title | Development of a clinical microarray system for genetic analysis screening |
| title_full | Development of a clinical microarray system for genetic analysis screening |
| title_fullStr | Development of a clinical microarray system for genetic analysis screening |
| title_full_unstemmed | Development of a clinical microarray system for genetic analysis screening |
| title_short | Development of a clinical microarray system for genetic analysis screening |
| title_sort | development of a clinical microarray system for genetic analysis screening |
| topic | Genetic analysis Microarray Medical care Screening test Surface treatment |
| url | http://www.sciencedirect.com/science/article/pii/S2352551722000452 |
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