Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications
We aim to develop the complex of ticlopidine hydrochloride (TCP) with β-cyclodextrin (β-CD) and to investigate its antibacterial and cytotoxic activities. The complex was characterized by various physicochemical as well as spectroscopic techniques suggesting the successful inclusion of TCP molecule...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Food Chemistry Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772753X22000016 |
| _version_ | 1811178655083134976 |
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| author | Pranish Bomzan Niloy Roy Vijeta Rai Debadrita Roy Shilpi Ghosh Anoop Kumar Kanak Roy Rinku Chakrabarty Jyotsna Das Vikas Kumar Dakua Kumar Basnet Mahendra Nath Roy |
| author_facet | Pranish Bomzan Niloy Roy Vijeta Rai Debadrita Roy Shilpi Ghosh Anoop Kumar Kanak Roy Rinku Chakrabarty Jyotsna Das Vikas Kumar Dakua Kumar Basnet Mahendra Nath Roy |
| author_sort | Pranish Bomzan |
| collection | DOAJ |
| description | We aim to develop the complex of ticlopidine hydrochloride (TCP) with β-cyclodextrin (β-CD) and to investigate its antibacterial and cytotoxic activities. The complex was characterized by various physicochemical as well as spectroscopic techniques suggesting the successful inclusion of TCP molecule into the β-CD cavity. TG analysis showed that the thermal stability of TCP was found to improve after complexation. Molecular docking study speculated the most preferred orientation of TCP molecule within the binding pocket of β-CD cavity. In vitro antibacterial activity test demonstrated that the TCP-β-CD complex displayed better activity than pure TCP. TCP-β-CD complex (IC50 = 24 μM) also exhibited significant in vitro cytotoxic activity than pure TCP (IC50 = 44 μM) towards human kidney cancer cell line (ACHN). Furthermore, the complex induces the ROS generation in ACHN cells more pronouncedly than TCP alone, suggesting the increased apoptotic activity of TCP after complexation. These results reveals that inclusion of TCP using β-CD could lead to stabilization of TCP and efficient display of its antibacterial and cytotoxic activities. |
| first_indexed | 2024-04-11T06:22:03Z |
| format | Article |
| id | doaj.art-aa38921a9eb24201be797e4b01e9364e |
| institution | Directory Open Access Journal |
| issn | 2772-753X |
| language | English |
| last_indexed | 2024-04-11T06:22:03Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry Advances |
| spelling | doaj.art-aa38921a9eb24201be797e4b01e9364e2022-12-22T04:40:32ZengElsevierFood Chemistry Advances2772-753X2022-10-011100015Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authenticationsPranish Bomzan0Niloy Roy1Vijeta Rai2Debadrita Roy3Shilpi Ghosh4Anoop Kumar5Kanak Roy6Rinku Chakrabarty7Jyotsna Das8Vikas Kumar Dakua9Kumar Basnet10Mahendra Nath Roy11Department of Chemistry, University of North Bengal, Raja Rammohanpur, Darjeeling, West Bengal 734013, IndiaDepartment of Chemistry, University of North Bengal, Raja Rammohanpur, Darjeeling, West Bengal 734013, IndiaDepartment of Biotechnology, University of North Bengal, Darjeeling 734013, IndiaDepartment of Chemistry, University of North Bengal, Raja Rammohanpur, Darjeeling, West Bengal 734013, IndiaDepartment of Biotechnology, University of North Bengal, Darjeeling 734013, IndiaDepartment of Biotechnology, University of North Bengal, Darjeeling 734013, IndiaDepartment of Chemistry, Alipurduar University, Alipurduar 736122, IndiaDepartment of Chemistry, Alipurduar University, Alipurduar 736122, IndiaDepartment of Botany, Alipurduar University, Alipurduar 736122, IndiaDepartment of Chemistry, Alipurduar University, Alipurduar 736122, IndiaDepartment of Zoology, Alipurduar University, Alipurduar 736122, IndiaCorresponding author at: Department of Chemistry, University of North Bengal, Raja Rammohanpur, Darjeeling, West Bengal 734013, India.; Department of Chemistry, University of North Bengal, Raja Rammohanpur, Darjeeling, West Bengal 734013, India; Department of Chemistry, Alipurduar University, Alipurduar 736122, IndiaWe aim to develop the complex of ticlopidine hydrochloride (TCP) with β-cyclodextrin (β-CD) and to investigate its antibacterial and cytotoxic activities. The complex was characterized by various physicochemical as well as spectroscopic techniques suggesting the successful inclusion of TCP molecule into the β-CD cavity. TG analysis showed that the thermal stability of TCP was found to improve after complexation. Molecular docking study speculated the most preferred orientation of TCP molecule within the binding pocket of β-CD cavity. In vitro antibacterial activity test demonstrated that the TCP-β-CD complex displayed better activity than pure TCP. TCP-β-CD complex (IC50 = 24 μM) also exhibited significant in vitro cytotoxic activity than pure TCP (IC50 = 44 μM) towards human kidney cancer cell line (ACHN). Furthermore, the complex induces the ROS generation in ACHN cells more pronouncedly than TCP alone, suggesting the increased apoptotic activity of TCP after complexation. These results reveals that inclusion of TCP using β-CD could lead to stabilization of TCP and efficient display of its antibacterial and cytotoxic activities.http://www.sciencedirect.com/science/article/pii/S2772753X22000016Ticlopidine hydrochlorideβ-cyclodextrinThermogravimetric analysisMolecular dockingAntibacterial activityCytotoxicity |
| spellingShingle | Pranish Bomzan Niloy Roy Vijeta Rai Debadrita Roy Shilpi Ghosh Anoop Kumar Kanak Roy Rinku Chakrabarty Jyotsna Das Vikas Kumar Dakua Kumar Basnet Mahendra Nath Roy Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications Food Chemistry Advances Ticlopidine hydrochloride β-cyclodextrin Thermogravimetric analysis Molecular docking Antibacterial activity Cytotoxicity |
| title | Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications |
| title_full | Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications |
| title_fullStr | Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications |
| title_full_unstemmed | Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications |
| title_short | Inclusion of an antiplatelet agent inside into β-cyclodextrin for biochemical applications with diverse authentications |
| title_sort | inclusion of an antiplatelet agent inside into β cyclodextrin for biochemical applications with diverse authentications |
| topic | Ticlopidine hydrochloride β-cyclodextrin Thermogravimetric analysis Molecular docking Antibacterial activity Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2772753X22000016 |
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