High-Level Production of Soluble Cross-Reacting Material 197 in <i>Escherichia coli</i> Cytoplasm Due to Fine Tuning of the Target Gene’s mRNA Structure

High-Level Production of Soluble Cross-Reacting Material 197 in <i>Escherichia coli</i> Cytoplasm Due to Fine Tuning of the Target Gene’s mRNA Structure

Cross-reacting material 197 (CRM197) is a non-toxic mutant of the diphtheria toxin and is widely used as a carrier protein in conjugate vaccines. This protein was first obtained from the supernatant of the mutant <i>Corynebacterium diphtheriae</i> strain. This pathogenic bacteria strain...

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Bibliographic Details
Main Authors: Yulia Alexandrovna Khodak, Alexandra Yurievna Ryazanova, Ivan Ivanovich Vorobiev, Alexander Leonidovich Kovalchuk, Nikolay Nikolaevich Ovechko, Petr Gennadievich Aparin
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:BioTech
Subjects:
CRM197
carrier protein
protein solubility
protein expression
<i>Escherichia coli</i>
SHuffle strain
Online Access:https://www.mdpi.com/2673-6284/12/1/9

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