Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract
AbstractType 2 diabetes (T2D) is a progressive metabolic disorder of glucose metabolism. One of the therapeutic approaches for the treatment of T2D is reducing postprandial hyperglycaemia through inhibition of the digestive enzymes α-glucosidase and α-amylase. In this context, aimed at identifying n...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/14756366.2023.2274798 |
| _version_ | 1797400963489726464 |
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| author | Cinzia Sanna Antonella Fais Benedetta Era Giovanna L. Delogu Enrico Sanna Laura Dazzi Antonella Rosa Arianna Marengo Patrizia Rubiolo Antonio De Agostini Sonia Floris Francesca Pintus |
| author_facet | Cinzia Sanna Antonella Fais Benedetta Era Giovanna L. Delogu Enrico Sanna Laura Dazzi Antonella Rosa Arianna Marengo Patrizia Rubiolo Antonio De Agostini Sonia Floris Francesca Pintus |
| author_sort | Cinzia Sanna |
| collection | DOAJ |
| description | AbstractType 2 diabetes (T2D) is a progressive metabolic disorder of glucose metabolism. One of the therapeutic approaches for the treatment of T2D is reducing postprandial hyperglycaemia through inhibition of the digestive enzymes α-glucosidase and α-amylase. In this context, aimed at identifying natural products endowed with anti-T2D potential, we focused on Ptilostemon casabonae (L.) Greuter, a species belonging to Asteraceae family. Enzymatic inhibition, antioxidant activity, phenolic composition and cellular assays were performed. This study revealed that the P. casabonae hydroalcoholic extract exerts a potent inhibitory activity against α-glucosidase. This activity is supported by an antioxidant effect, preventing ROS formation in a stressed cellular system. HPLC-PDA-MS/MS analysis, revealed a complex polyphenolic fraction. Among the tested pure compounds, 1,5-dicaffeoylquinic acid, apigenin and rutin displayed good α-glucosidase inhibitory activity. Our study suggested new potential of P. casabonae encouraging us to further testing the possible therapeutic potential of this extract. |
| first_indexed | 2024-03-09T02:03:05Z |
| format | Article |
| id | doaj.art-aa394a58609849ec92d4e0dba336dc05 |
| institution | Directory Open Access Journal |
| issn | 1475-6366 1475-6374 |
| language | English |
| last_indexed | 2024-03-09T02:03:05Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj.art-aa394a58609849ec92d4e0dba336dc052023-12-08T03:24:20ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742023-12-0138110.1080/14756366.2023.2274798Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extractCinzia Sanna0Antonella Fais1Benedetta Era2Giovanna L. Delogu3Enrico Sanna4Laura Dazzi5Antonella Rosa6Arianna Marengo7Patrizia Rubiolo8Antonio De Agostini9Sonia Floris10Francesca Pintus11Department of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Biomedical Science, University of Cagliari, Cagliari, ItalyDepartment of Drug Science and Technology, University of Turin, Turin, ItalyDepartment of Drug Science and Technology, University of Turin, Turin, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, Cagliari, ItalyAbstractType 2 diabetes (T2D) is a progressive metabolic disorder of glucose metabolism. One of the therapeutic approaches for the treatment of T2D is reducing postprandial hyperglycaemia through inhibition of the digestive enzymes α-glucosidase and α-amylase. In this context, aimed at identifying natural products endowed with anti-T2D potential, we focused on Ptilostemon casabonae (L.) Greuter, a species belonging to Asteraceae family. Enzymatic inhibition, antioxidant activity, phenolic composition and cellular assays were performed. This study revealed that the P. casabonae hydroalcoholic extract exerts a potent inhibitory activity against α-glucosidase. This activity is supported by an antioxidant effect, preventing ROS formation in a stressed cellular system. HPLC-PDA-MS/MS analysis, revealed a complex polyphenolic fraction. Among the tested pure compounds, 1,5-dicaffeoylquinic acid, apigenin and rutin displayed good α-glucosidase inhibitory activity. Our study suggested new potential of P. casabonae encouraging us to further testing the possible therapeutic potential of this extract.https://www.tandfonline.com/doi/10.1080/14756366.2023.2274798α-Glucosidaseα-amylasediabetesenzyme inhibitionPtilostemon casabonae |
| spellingShingle | Cinzia Sanna Antonella Fais Benedetta Era Giovanna L. Delogu Enrico Sanna Laura Dazzi Antonella Rosa Arianna Marengo Patrizia Rubiolo Antonio De Agostini Sonia Floris Francesca Pintus Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract Journal of Enzyme Inhibition and Medicinal Chemistry α-Glucosidase α-amylase diabetes enzyme inhibition Ptilostemon casabonae |
| title | Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract |
| title_full | Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract |
| title_fullStr | Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract |
| title_full_unstemmed | Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract |
| title_short | Promising inhibition of diabetes-related enzymes and antioxidant properties of Ptilostemon casabonae leaves extract |
| title_sort | promising inhibition of diabetes related enzymes and antioxidant properties of ptilostemon casabonae leaves extract |
| topic | α-Glucosidase α-amylase diabetes enzyme inhibition Ptilostemon casabonae |
| url | https://www.tandfonline.com/doi/10.1080/14756366.2023.2274798 |
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