The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system
Environmental bacteria are endowed with several regulatory systems that have potential applications in biotechnology. In this report, we characterize the arsenic biosensing features of the ars response system from Chromobacterium violaceum in the heterologous host Escherichia coli. We show that the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01851/full |
| _version_ | 1818541199372320768 |
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| author | Letícia Magalhães Arruda Lummy Maria Oliveira Monteiro Rafael Silva-Rocha |
| author_facet | Letícia Magalhães Arruda Lummy Maria Oliveira Monteiro Rafael Silva-Rocha |
| author_sort | Letícia Magalhães Arruda |
| collection | DOAJ |
| description | Environmental bacteria are endowed with several regulatory systems that have potential applications in biotechnology. In this report, we characterize the arsenic biosensing features of the ars response system from Chromobacterium violaceum in the heterologous host Escherichia coli. We show that the native Pars/arsR system of C. violaceum outperforms the chromosomal ars copy of E. coli when exposed to micromolar concentrations of arsenite. To understand the molecular basis of this phenomenon, we analyzed the interaction between ArsR regulators and their promoter target sites as well as induction of the system at saturating concentrations of the regulators. In vivo titration experiments indicate that ArsR from C. violaceum has stronger binding affinity for its target promoter than the regulator from E. coli does. Additionally, arsenite induction experiments at saturating regulator concentration demonstrates that although the Pars/arsR system from E. coli displays a gradual response to increasing concentration of the inducer, the system from C. violaceum has a steeper response with a stronger promoter induction after a given arsenite threshold. Taken together, these data demonstrate the characterization of a novel arsenic response element from an environmental bacterium with potentially enhanced performance that could be further explored for the construction of an arsenic biosensor. |
| first_indexed | 2024-12-11T22:05:28Z |
| format | Article |
| id | doaj.art-aa39b5894d5b4dbc808d8c9dda9506f6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T22:05:28Z |
| publishDate | 2016-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-aa39b5894d5b4dbc808d8c9dda9506f62022-12-22T00:48:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-11-01710.3389/fmicb.2016.01851227737The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli systemLetícia Magalhães Arruda0Lummy Maria Oliveira Monteiro1Rafael Silva-Rocha2FMRP-USPFMRP-USPFMRP-USPEnvironmental bacteria are endowed with several regulatory systems that have potential applications in biotechnology. In this report, we characterize the arsenic biosensing features of the ars response system from Chromobacterium violaceum in the heterologous host Escherichia coli. We show that the native Pars/arsR system of C. violaceum outperforms the chromosomal ars copy of E. coli when exposed to micromolar concentrations of arsenite. To understand the molecular basis of this phenomenon, we analyzed the interaction between ArsR regulators and their promoter target sites as well as induction of the system at saturating concentrations of the regulators. In vivo titration experiments indicate that ArsR from C. violaceum has stronger binding affinity for its target promoter than the regulator from E. coli does. Additionally, arsenite induction experiments at saturating regulator concentration demonstrates that although the Pars/arsR system from E. coli displays a gradual response to increasing concentration of the inducer, the system from C. violaceum has a steeper response with a stronger promoter induction after a given arsenite threshold. Taken together, these data demonstrate the characterization of a novel arsenic response element from an environmental bacterium with potentially enhanced performance that could be further explored for the construction of an arsenic biosensor.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01851/fullregulatory networkars operoncis-regulatory elementsarsenic response systemArsR/SmtB family |
| spellingShingle | Letícia Magalhães Arruda Lummy Maria Oliveira Monteiro Rafael Silva-Rocha The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system Frontiers in Microbiology regulatory network ars operon cis-regulatory elements arsenic response system ArsR/SmtB family |
| title | The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system |
| title_full | The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system |
| title_fullStr | The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system |
| title_full_unstemmed | The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system |
| title_short | The Chromobacterium violaceum ArsR arsenite repressor exerts tighter control on its cognate promoter than the Escherichia coli system |
| title_sort | chromobacterium violaceum arsr arsenite repressor exerts tighter control on its cognate promoter than the escherichia coli system |
| topic | regulatory network ars operon cis-regulatory elements arsenic response system ArsR/SmtB family |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01851/full |
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