Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing
Lakes are currently responsible for a significant amount of total natural methane emission. Microbial oxidation of methane plays a central role in Arctic carbon cycling, potentially reducing methane emissions from lakes, though little is known about methane cycling in the water column of Arctic lake...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Environmental Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fenvs.2022.884133/full |
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| author | Sarah B. Cadieux Sarah B. Cadieux Ursel M. E. Schütte Chris Hemmerich Sarah Powers Jeffrey R. White Jeffrey R. White |
| author_facet | Sarah B. Cadieux Sarah B. Cadieux Ursel M. E. Schütte Chris Hemmerich Sarah Powers Jeffrey R. White Jeffrey R. White |
| author_sort | Sarah B. Cadieux |
| collection | DOAJ |
| description | Lakes are currently responsible for a significant amount of total natural methane emission. Microbial oxidation of methane plays a central role in Arctic carbon cycling, potentially reducing methane emissions from lakes, though little is known about methane cycling in the water column of Arctic lakes. We previously detected surprisingly large enrichments of heavy carbon and hydrogen isotopes of methane in three small lakes in Greenland suggesting unusually efficient methanotrophic communities in these Arctic lakes. Using stable isotope and 16S rRNA gene sequencing we determined carbon and hydrogen isotopes and microbial community composition down the water column of Teardrop lake, under open-water conditions. We found that isotopic values of methane in Teardrop lake were again highly enriched 13C and 2H at 4 m depth with −13.2‰ and −27.1‰ values for carbon and hydrogen isotopes, respectively. Methane concentrations slightly increased at the depth interval with isotope enrichment, not typical of classic methanotrophy. Consistent with isotopic enrichment of the heavy isotopes we detected the highest relative abundance of putative methanotrophs, in particular Methylovulum at 4 m. The highest relative abundance of putative methanogens was detected at 3 m as well as at 5 m. At the same depth interval, temperature and oxidation reduction potential also increase, supporting increased microbial activity within the water column. Based on geochemical and microbial observations, we suggest that the methane cycling in Teardrop lake is decoupled from a traditional depth dependent model where the dominant source of methane is in the anoxic sediments. Instead, methane in the water column is likely from a combination of anoxic sediment, littoral transport and oxic methanogenesis in the mid-water column, and recycling of carbon within the water column is leading to extreme isotope enrichments. Thus, understanding linkages between depth-dependent microbial dynamics and methane biogeochemistry are necessary to constrain the sensitivity of the methane cycle to future climate change. |
| first_indexed | 2024-04-11T19:50:38Z |
| format | Article |
| id | doaj.art-aa39b8f1365b4646b55ad164dc8b78bf |
| institution | Directory Open Access Journal |
| issn | 2296-665X |
| language | English |
| last_indexed | 2024-04-11T19:50:38Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Environmental Science |
| spelling | doaj.art-aa39b8f1365b4646b55ad164dc8b78bf2022-12-22T04:06:20ZengFrontiers Media S.A.Frontiers in Environmental Science2296-665X2022-10-011010.3389/fenvs.2022.884133884133Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencingSarah B. Cadieux0Sarah B. Cadieux1Ursel M. E. Schütte2Chris Hemmerich3Sarah Powers4Jeffrey R. White5Jeffrey R. White6Earth and Environmental Sciences, Rensselaer Polytechnic Institute, Troy, NY, United StatesEarth and Atmospheric Sciences, Indiana University, Bloomington, IN, United StatesInstitute for Arctic Biology, University of Alaska Fairbanks, Fairbanks, AK, United StatesCenter for Genomics and Bioinformatics, Indiana University, Bloomington, IN, United StatesO’Neill School of Public and Environmental Affairs, Indiana University, Bloomington, IN, United StatesEarth and Atmospheric Sciences, Indiana University, Bloomington, IN, United StatesO’Neill School of Public and Environmental Affairs, Indiana University, Bloomington, IN, United StatesLakes are currently responsible for a significant amount of total natural methane emission. Microbial oxidation of methane plays a central role in Arctic carbon cycling, potentially reducing methane emissions from lakes, though little is known about methane cycling in the water column of Arctic lakes. We previously detected surprisingly large enrichments of heavy carbon and hydrogen isotopes of methane in three small lakes in Greenland suggesting unusually efficient methanotrophic communities in these Arctic lakes. Using stable isotope and 16S rRNA gene sequencing we determined carbon and hydrogen isotopes and microbial community composition down the water column of Teardrop lake, under open-water conditions. We found that isotopic values of methane in Teardrop lake were again highly enriched 13C and 2H at 4 m depth with −13.2‰ and −27.1‰ values for carbon and hydrogen isotopes, respectively. Methane concentrations slightly increased at the depth interval with isotope enrichment, not typical of classic methanotrophy. Consistent with isotopic enrichment of the heavy isotopes we detected the highest relative abundance of putative methanotrophs, in particular Methylovulum at 4 m. The highest relative abundance of putative methanogens was detected at 3 m as well as at 5 m. At the same depth interval, temperature and oxidation reduction potential also increase, supporting increased microbial activity within the water column. Based on geochemical and microbial observations, we suggest that the methane cycling in Teardrop lake is decoupled from a traditional depth dependent model where the dominant source of methane is in the anoxic sediments. Instead, methane in the water column is likely from a combination of anoxic sediment, littoral transport and oxic methanogenesis in the mid-water column, and recycling of carbon within the water column is leading to extreme isotope enrichments. Thus, understanding linkages between depth-dependent microbial dynamics and methane biogeochemistry are necessary to constrain the sensitivity of the methane cycle to future climate change.https://www.frontiersin.org/articles/10.3389/fenvs.2022.884133/fullmethane oxidationmethanotrophsisotopeslakearcticmethane cycling |
| spellingShingle | Sarah B. Cadieux Sarah B. Cadieux Ursel M. E. Schütte Chris Hemmerich Sarah Powers Jeffrey R. White Jeffrey R. White Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing Frontiers in Environmental Science methane oxidation methanotrophs isotopes lake arctic methane cycling |
| title | Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing |
| title_full | Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing |
| title_fullStr | Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing |
| title_full_unstemmed | Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing |
| title_short | Exploring methane cycling in an arctic lake in Kangerlussuaq Greenland using stable isotopes and 16S rRNA gene sequencing |
| title_sort | exploring methane cycling in an arctic lake in kangerlussuaq greenland using stable isotopes and 16s rrna gene sequencing |
| topic | methane oxidation methanotrophs isotopes lake arctic methane cycling |
| url | https://www.frontiersin.org/articles/10.3389/fenvs.2022.884133/full |
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