COMPARISON BETWEEN TWO-LAYER DENSITY GRADIENT AND THREE-LAYER DENSITY GRADIENT TECHNIQUE FOR SPERM PREPARATION AT ASTER FERTILITY CLINIC, DR. HASAN SADIKIN GENERAL HOSPITAL
Objective: To compare the efficacy of sperm preparation techniques modified by two layer density gradient technique (80%, 40%). Methods: Sperm preparation was performed by two- and three-layer density gradient technique by centrifugation at 500 rpm for 20 minutes using SpermGrad (Vitrolife, Swe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Padjadjaran
2014-06-01
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| Series: | International Journal of Integrated Health Sciences |
| Subjects: | |
| Online Access: | http://journal.fk.unpad.ac.id/index.php/ijihs/article/view/286/282 |
| Summary: | Objective: To compare the efficacy of sperm preparation techniques modified by two layer density gradient technique (80%, 40%).
Methods: Sperm preparation was performed by two- and three-layer density gradient technique by centrifugation at 500 rpm for 20 minutes using SpermGrad (Vitrolife, Sweden).
Results: The average concentration of total sperm preparation by two-layer density gradient was 23.19 million with a standard deviation of 11.9 million while the result of three-layer density gradient preparation obtained a mean total sperm concentration of 19.64 million with a standard deviation of 10.033 million. The mean total concentration of sperm in three-layer was lower than two-layer density gradient with p <0.001.
Conclusions: This study concludes that there was a significant difference between the concentration of sperm prepared using two-layer and three-layers density gradient technique. |
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| ISSN: | 2302-1381 2338-4506 |