Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa.
Pseudomonas aeruginosa is a leading pathogen that has become increasingly resistant to the fluoroquinolone antibiotics due to widespread prescribing. Adverse outcomes have been shown for patients infected with fluoroquinolone-resistant strains. The type III secretion system (TTSS) is a major virulen...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3414457?pdf=render |
| _version_ | 1828424934217482240 |
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| author | Melissa Agnello Annie Wong-Beringer |
| author_facet | Melissa Agnello Annie Wong-Beringer |
| author_sort | Melissa Agnello |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a leading pathogen that has become increasingly resistant to the fluoroquinolone antibiotics due to widespread prescribing. Adverse outcomes have been shown for patients infected with fluoroquinolone-resistant strains. The type III secretion system (TTSS) is a major virulence determinant during acute infections through the injection of effector toxins into host cells. Most strains exhibit a unique TTSS virulence genotype defined by the presence of either exoS or exoU gene encoding two of the effector toxins, ExoS and ExoU, respectively. Specific TTSS effector genotype has been shown previously to differentially impact virulence in pneumonia. In this study, we examined the relationship between TTSS effector genotype and fluoroquinolone resistance mechanisms in a collection of 270 respiratory isolates. We found that a higher proportion of exoU+ strains were fluoroquinolone-resistant compared to exoS+ strains (63% vs 49%, p = 0.03) despite its lower overall prevalence (38% exoU+ vs 56% exoS+). Results from sequencing the quinolone resistance determining regions (QRDRs) of the 4 target genes (gyrA, gyrB, parC, parE) indicated that strains containing the exoU gene were more likely to acquire ≥ 2 mutations than exoS+ strains at MICs ≤ 8 µg/ml (13% vs none) and twice as likely to have mutations in both gyrA and parC than exoS+ strains (48% vs 24% p = 0.0439). Our findings indicate that P. aeruginosa strains differentially develop resistance-conferring mutations that correlate with TTSS effector genotype and the more virulent exoU+ subpopulation. Differences in mutational processes by virulence genotype that were observed suggest co-evolution of resistance and virulence traits favoring a more virulent genotype in the quinolone-rich clinical environment. |
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| id | doaj.art-aa3a2ed1d7a94bd5984a6ed31a47cb64 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T16:24:01Z |
| publishDate | 2012-01-01 |
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| series | PLoS ONE |
| spelling | doaj.art-aa3a2ed1d7a94bd5984a6ed31a47cb642022-12-22T01:41:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4297310.1371/journal.pone.0042973Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa.Melissa AgnelloAnnie Wong-BeringerPseudomonas aeruginosa is a leading pathogen that has become increasingly resistant to the fluoroquinolone antibiotics due to widespread prescribing. Adverse outcomes have been shown for patients infected with fluoroquinolone-resistant strains. The type III secretion system (TTSS) is a major virulence determinant during acute infections through the injection of effector toxins into host cells. Most strains exhibit a unique TTSS virulence genotype defined by the presence of either exoS or exoU gene encoding two of the effector toxins, ExoS and ExoU, respectively. Specific TTSS effector genotype has been shown previously to differentially impact virulence in pneumonia. In this study, we examined the relationship between TTSS effector genotype and fluoroquinolone resistance mechanisms in a collection of 270 respiratory isolates. We found that a higher proportion of exoU+ strains were fluoroquinolone-resistant compared to exoS+ strains (63% vs 49%, p = 0.03) despite its lower overall prevalence (38% exoU+ vs 56% exoS+). Results from sequencing the quinolone resistance determining regions (QRDRs) of the 4 target genes (gyrA, gyrB, parC, parE) indicated that strains containing the exoU gene were more likely to acquire ≥ 2 mutations than exoS+ strains at MICs ≤ 8 µg/ml (13% vs none) and twice as likely to have mutations in both gyrA and parC than exoS+ strains (48% vs 24% p = 0.0439). Our findings indicate that P. aeruginosa strains differentially develop resistance-conferring mutations that correlate with TTSS effector genotype and the more virulent exoU+ subpopulation. Differences in mutational processes by virulence genotype that were observed suggest co-evolution of resistance and virulence traits favoring a more virulent genotype in the quinolone-rich clinical environment.http://europepmc.org/articles/PMC3414457?pdf=render |
| spellingShingle | Melissa Agnello Annie Wong-Beringer Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. PLoS ONE |
| title | Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. |
| title_full | Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. |
| title_fullStr | Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. |
| title_full_unstemmed | Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. |
| title_short | Differentiation in quinolone resistance by virulence genotype in Pseudomonas aeruginosa. |
| title_sort | differentiation in quinolone resistance by virulence genotype in pseudomonas aeruginosa |
| url | http://europepmc.org/articles/PMC3414457?pdf=render |
| work_keys_str_mv | AT melissaagnello differentiationinquinoloneresistancebyvirulencegenotypeinpseudomonasaeruginosa AT anniewongberinger differentiationinquinoloneresistancebyvirulencegenotypeinpseudomonasaeruginosa |