Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma
Previous reports have shown that miR‐30d‐5p functions as a tumor suppressor in prostate cancer and gallbladder carcinoma, but its role in renal cell carcinoma (RCC) remains elusive. This study was designed to explore the functional role of miR‐30d‐5p in proliferation and autophagy of RCC. Our result...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-02-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13025 |
| _version_ | 1811299567042297856 |
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| author | Liang Liang Zheng Yang Qian Deng Yazhuo Jiang Yongyi Cheng Yi Sun Lei Li |
| author_facet | Liang Liang Zheng Yang Qian Deng Yazhuo Jiang Yongyi Cheng Yi Sun Lei Li |
| author_sort | Liang Liang |
| collection | DOAJ |
| description | Previous reports have shown that miR‐30d‐5p functions as a tumor suppressor in prostate cancer and gallbladder carcinoma, but its role in renal cell carcinoma (RCC) remains elusive. This study was designed to explore the functional role of miR‐30d‐5p in proliferation and autophagy of RCC. Our results show that miR‐30d‐5p is significantly down‐regulated in RCC tissues compared with normal tissues. miR‐30d‐5p overexpression suppressed cell proliferation, cell‐cycle G1/S transition and autophagy, but promoted apoptosis in RCC cell lines (786‐O and ACHN). Intriguingly, autophagy‐related gene 5 (ATG5) was directly targeted by miR‐30d‐5p, as shown using luciferase reporter assay and biotin‐avidin pull‐down assay. Moreover, overexpression of ATG5 attenuated the inhibitory effect of miR‐30d‐5p on proliferation and autophagy in 786‐O cells. These results suggest that miR‐30d‐5p suppresses proliferation and autophagy in RCC cells by targeting ATG5, and this pathway may be a suitable basis for the design of novel cancer therapeutics. |
| first_indexed | 2024-04-13T06:37:46Z |
| format | Article |
| id | doaj.art-aa4265f0b8944eadadf5ec6d4ccad0e5 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-13T06:37:46Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-aa4265f0b8944eadadf5ec6d4ccad0e52022-12-22T02:57:51ZengWileyFEBS Open Bio2211-54632021-02-0111252954010.1002/2211-5463.13025Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinomaLiang Liang0Zheng Yang1Qian Deng2Yazhuo Jiang3Yongyi Cheng4Yi Sun5Lei Li6Department of Urology The First Affiliated Hospital of Xi'an Jiaotong University ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University ChinaDepartment of Urology Shaanxi Provincial People's Hospital Xi'an ChinaDepartment of Urology Shaanxi Provincial People's Hospital Xi'an ChinaDepartment of Urology Shaanxi Provincial People's Hospital Xi'an ChinaDepartment of Urology Shaanxi Provincial People's Hospital Xi'an ChinaDepartment of Urology The First Affiliated Hospital of Xi'an Jiaotong University ChinaPrevious reports have shown that miR‐30d‐5p functions as a tumor suppressor in prostate cancer and gallbladder carcinoma, but its role in renal cell carcinoma (RCC) remains elusive. This study was designed to explore the functional role of miR‐30d‐5p in proliferation and autophagy of RCC. Our results show that miR‐30d‐5p is significantly down‐regulated in RCC tissues compared with normal tissues. miR‐30d‐5p overexpression suppressed cell proliferation, cell‐cycle G1/S transition and autophagy, but promoted apoptosis in RCC cell lines (786‐O and ACHN). Intriguingly, autophagy‐related gene 5 (ATG5) was directly targeted by miR‐30d‐5p, as shown using luciferase reporter assay and biotin‐avidin pull‐down assay. Moreover, overexpression of ATG5 attenuated the inhibitory effect of miR‐30d‐5p on proliferation and autophagy in 786‐O cells. These results suggest that miR‐30d‐5p suppresses proliferation and autophagy in RCC cells by targeting ATG5, and this pathway may be a suitable basis for the design of novel cancer therapeutics.https://doi.org/10.1002/2211-5463.13025ATG5autophagymiR‐30d‐5pproliferationrenal cell carcinoma |
| spellingShingle | Liang Liang Zheng Yang Qian Deng Yazhuo Jiang Yongyi Cheng Yi Sun Lei Li Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma FEBS Open Bio ATG5 autophagy miR‐30d‐5p proliferation renal cell carcinoma |
| title | Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma |
| title_full | Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma |
| title_fullStr | Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma |
| title_full_unstemmed | Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma |
| title_short | Retracted: miR‐30d‐5p suppresses proliferation and autophagy by targeting ATG5 in renal cell carcinoma |
| title_sort | retracted mir 30d 5p suppresses proliferation and autophagy by targeting atg5 in renal cell carcinoma |
| topic | ATG5 autophagy miR‐30d‐5p proliferation renal cell carcinoma |
| url | https://doi.org/10.1002/2211-5463.13025 |
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