The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment
The aims of the present study were to investigate the anti-inflammatory function of two flavonoids apigenin and genistein in rat intestinal epithelial (IEC-6) cells stimulated by tumor necrosis factor-alpha (TNF-α) and to clarify whether the heat treatment of the flavonoids might affect flavonoid ac...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Food Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927122000831 |
| _version_ | 1811202246411550720 |
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| author | Shi-Qing Cai Zhi-Mei Tang Cen Xiong Fei-Fei Wu Jun-Ren Zhao Qiang Zhang Li Wang Xiao-Nan Zhang Xin-Huai Zhao |
| author_facet | Shi-Qing Cai Zhi-Mei Tang Cen Xiong Fei-Fei Wu Jun-Ren Zhao Qiang Zhang Li Wang Xiao-Nan Zhang Xin-Huai Zhao |
| author_sort | Shi-Qing Cai |
| collection | DOAJ |
| description | The aims of the present study were to investigate the anti-inflammatory function of two flavonoids apigenin and genistein in rat intestinal epithelial (IEC-6) cells stimulated by tumor necrosis factor-alpha (TNF-α) and to clarify whether the heat treatment of the flavonoids might affect flavonoid activity. The flavonoids at lower dosage (e.g. 5 μmol/L) had no toxic effect but growth promotion on the cells. Meanwhile, the flavonoid pretreatment of the cells before TNF-α stimulation could maintain cellular morphology, decrease the production of prostaglandin E2 and two pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-6, but increase the production of two anti-inflammatory cytokines IL-10 and transforming growth factor-β. Additionally, the flavonoids could block off the nuclear translocation of nuclear factor-kappaB (NF-κB) p65, and suppress the expression of phosphorylated IκBα and p65 induced by TNF-α. Meanwhile, the NF-κB inhibitor BAY 11–7082 shared a similar function with the flavonoids to mediate the production of IL-6/IL-10. Furthermore, in silico analysis also declared that the flavonoids could interact with the IκBα–NF–κB complex at the binding pockets to yield the binding energies ranging from −31.7 to −34.0 kJ/mol. However, the heated flavonoids were consistently less effective than the unheated counterparts to perform these anti-inflammatory effects. It is thus proposed that both apigenin and genistein have anti-inflammatory potential to the TNF-α-stimulated IEC-6 cells by inactivating the NF-κB pathway, while heat treatment of the flavonoids caused a negative impact on these assessed anti-inflammatory effects. |
| first_indexed | 2024-04-12T02:35:46Z |
| format | Article |
| id | doaj.art-aa439490152547609be043f863567274 |
| institution | Directory Open Access Journal |
| issn | 2665-9271 |
| language | English |
| last_indexed | 2024-04-12T02:35:46Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj.art-aa439490152547609be043f8635672742022-12-22T03:51:34ZengElsevierCurrent Research in Food Science2665-92712022-01-015918926The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatmentShi-Qing Cai0Zhi-Mei Tang1Cen Xiong2Fei-Fei Wu3Jun-Ren Zhao4Qiang Zhang5Li Wang6Xiao-Nan Zhang7Xin-Huai Zhao8School of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR ChinaSchool of Life Science, Jiaying University, 514015, Meizhou, PR ChinaSchool of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Research Centre of Food Nutrition and Human Healthcare, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China; Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, 150030, Harbin, PR China; Corresponding author. School of Biology and Food Engineering, Guangdong University of Petrochemical Technology, 525000, Maoming, PR China.The aims of the present study were to investigate the anti-inflammatory function of two flavonoids apigenin and genistein in rat intestinal epithelial (IEC-6) cells stimulated by tumor necrosis factor-alpha (TNF-α) and to clarify whether the heat treatment of the flavonoids might affect flavonoid activity. The flavonoids at lower dosage (e.g. 5 μmol/L) had no toxic effect but growth promotion on the cells. Meanwhile, the flavonoid pretreatment of the cells before TNF-α stimulation could maintain cellular morphology, decrease the production of prostaglandin E2 and two pro-inflammatory cytokines interleukin-1β (IL-1β) and IL-6, but increase the production of two anti-inflammatory cytokines IL-10 and transforming growth factor-β. Additionally, the flavonoids could block off the nuclear translocation of nuclear factor-kappaB (NF-κB) p65, and suppress the expression of phosphorylated IκBα and p65 induced by TNF-α. Meanwhile, the NF-κB inhibitor BAY 11–7082 shared a similar function with the flavonoids to mediate the production of IL-6/IL-10. Furthermore, in silico analysis also declared that the flavonoids could interact with the IκBα–NF–κB complex at the binding pockets to yield the binding energies ranging from −31.7 to −34.0 kJ/mol. However, the heated flavonoids were consistently less effective than the unheated counterparts to perform these anti-inflammatory effects. It is thus proposed that both apigenin and genistein have anti-inflammatory potential to the TNF-α-stimulated IEC-6 cells by inactivating the NF-κB pathway, while heat treatment of the flavonoids caused a negative impact on these assessed anti-inflammatory effects.http://www.sciencedirect.com/science/article/pii/S2665927122000831ApigeninGenisteinIEC-6 cellsInflammationHeat treatmentNF-κB pathway |
| spellingShingle | Shi-Qing Cai Zhi-Mei Tang Cen Xiong Fei-Fei Wu Jun-Ren Zhao Qiang Zhang Li Wang Xiao-Nan Zhang Xin-Huai Zhao The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment Current Research in Food Science Apigenin Genistein IEC-6 cells Inflammation Heat treatment NF-κB pathway |
| title | The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment |
| title_full | The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment |
| title_fullStr | The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment |
| title_full_unstemmed | The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment |
| title_short | The anti-inflammatory effects of apigenin and genistein on the rat intestinal epithelial (IEC-6) cells with TNF-α stimulation in response to heat treatment |
| title_sort | anti inflammatory effects of apigenin and genistein on the rat intestinal epithelial iec 6 cells with tnf α stimulation in response to heat treatment |
| topic | Apigenin Genistein IEC-6 cells Inflammation Heat treatment NF-κB pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2665927122000831 |
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