Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles
DNA is a building block of life; surface-enhanced Raman spectroscopy (SERS) has been broadly applied in the detection of biomolecules but there are challenges in obtaining high-quality DNA SERS signals under non-destructive conditions. Here, we developed a novel label-free approach for DNA detection...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/12/18/3119 |
| _version_ | 1827658262506373120 |
|---|---|
| author | Ting Zhang Xubin Quan Naisi Cao Zhaoying Zhang Yang Li |
| author_facet | Ting Zhang Xubin Quan Naisi Cao Zhaoying Zhang Yang Li |
| author_sort | Ting Zhang |
| collection | DOAJ |
| description | DNA is a building block of life; surface-enhanced Raman spectroscopy (SERS) has been broadly applied in the detection of biomolecules but there are challenges in obtaining high-quality DNA SERS signals under non-destructive conditions. Here, we developed a novel label-free approach for DNA detection based on SERS, in which the Au@AgNPs core–shell structure was selected as the enhancement substrate, which not only solved the problem of the weak enhancement effect of gold nanoparticles but also overcame the disadvantage of the inhomogeneous shapes of silver nanoparticles, thereby improving the sensitivity and reproducibility of the SERS signals of DNA molecules. The method obtained SERS signals for four DNA bases (A, C, G, and T) without destroying the structure, then further detected and qualified different specific structures of DNA molecules. These results promote the application of SERS technology in the field of biomolecular detection. |
| first_indexed | 2024-03-09T22:59:16Z |
| format | Article |
| id | doaj.art-aa44a702f11440a892308eb09678cfd4 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T22:59:16Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-aa44a702f11440a892308eb09678cfd42023-11-23T18:05:46ZengMDPI AGNanomaterials2079-49912022-09-011218311910.3390/nano12183119Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag NanoparticlesTing Zhang0Xubin Quan1Naisi Cao2Zhaoying Zhang3Yang Li4Department of Pharmaceutical Analysis and Analytical Chemistry, Research Center for Innovative Technology of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Harbin 150081, ChinaDepartment of Pharmaceutical Analysis and Analytical Chemistry, Research Center for Innovative Technology of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Harbin 150081, ChinaDepartment of Pharmaceutical Analysis and Analytical Chemistry, Research Center for Innovative Technology of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Harbin 150081, ChinaThe Fourth Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Pharmaceutical Analysis and Analytical Chemistry, Research Center for Innovative Technology of Pharmaceutical Analysis, College of Pharmacy, Harbin Medical University, Harbin 150081, ChinaDNA is a building block of life; surface-enhanced Raman spectroscopy (SERS) has been broadly applied in the detection of biomolecules but there are challenges in obtaining high-quality DNA SERS signals under non-destructive conditions. Here, we developed a novel label-free approach for DNA detection based on SERS, in which the Au@AgNPs core–shell structure was selected as the enhancement substrate, which not only solved the problem of the weak enhancement effect of gold nanoparticles but also overcame the disadvantage of the inhomogeneous shapes of silver nanoparticles, thereby improving the sensitivity and reproducibility of the SERS signals of DNA molecules. The method obtained SERS signals for four DNA bases (A, C, G, and T) without destroying the structure, then further detected and qualified different specific structures of DNA molecules. These results promote the application of SERS technology in the field of biomolecular detection.https://www.mdpi.com/2079-4991/12/18/3119surface-enhanced Raman spectroscopyAu@Ag nanoparticlesDNAlabel-free detection |
| spellingShingle | Ting Zhang Xubin Quan Naisi Cao Zhaoying Zhang Yang Li Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles Nanomaterials surface-enhanced Raman spectroscopy Au@Ag nanoparticles DNA label-free detection |
| title | Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles |
| title_full | Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles |
| title_fullStr | Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles |
| title_full_unstemmed | Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles |
| title_short | Label-Free Detection of DNA via Surface-Enhanced Raman Spectroscopy Using Au@Ag Nanoparticles |
| title_sort | label free detection of dna via surface enhanced raman spectroscopy using au ag nanoparticles |
| topic | surface-enhanced Raman spectroscopy Au@Ag nanoparticles DNA label-free detection |
| url | https://www.mdpi.com/2079-4991/12/18/3119 |
| work_keys_str_mv | AT tingzhang labelfreedetectionofdnaviasurfaceenhancedramanspectroscopyusingauagnanoparticles AT xubinquan labelfreedetectionofdnaviasurfaceenhancedramanspectroscopyusingauagnanoparticles AT naisicao labelfreedetectionofdnaviasurfaceenhancedramanspectroscopyusingauagnanoparticles AT zhaoyingzhang labelfreedetectionofdnaviasurfaceenhancedramanspectroscopyusingauagnanoparticles AT yangli labelfreedetectionofdnaviasurfaceenhancedramanspectroscopyusingauagnanoparticles |