Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons
Abstract Targeted manipulation of neural activity via light has become an indispensable tool for gaining insights into the intricate processes governing single neurons and complex neural networks. To shed light onto the underlying interaction mechanisms, it is crucial to achieve precise control of i...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-03-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202304561 |
| _version_ | 1797242703177580544 |
|---|---|
| author | Corinna Kaspar Alexander Ivanenko Julia Lehrich Jürgen Klingauf Wolfram H.P. Pernice |
| author_facet | Corinna Kaspar Alexander Ivanenko Julia Lehrich Jürgen Klingauf Wolfram H.P. Pernice |
| author_sort | Corinna Kaspar |
| collection | DOAJ |
| description | Abstract Targeted manipulation of neural activity via light has become an indispensable tool for gaining insights into the intricate processes governing single neurons and complex neural networks. To shed light onto the underlying interaction mechanisms, it is crucial to achieve precise control of individual neural activity, as well as a spatial read‐out resolution on the nanoscale. Here, a versatile photonic platform with subcellular resolution for stimulation and monitoring of in‐vitro neurons is demonstrated. Low‐loss photonic waveguides are fabricated on glass substrates using nanoimprint lithography and featuring a loss of only ‐0.9 ± 0.2 dB cm−1 at 489 nm and are combined with optical fiber‐based waveguide‐access and backside total internal reflection fluorescence microscopy. Neurons are grown on the bio‐functionalized photonic chip surface and, expressing the light‐sensitive ion channel Channelrhodopsin‐2, are stimulated within the evanescent field penetration depth of 57 nm of the biocompatible waveguides. The versatility and cost‐efficiency of the platform, along with the possible subcellular resolution, enable tailor‐made investigations of neural interaction dynamics with defined spatial control and high throughput. |
| first_indexed | 2024-04-24T18:43:26Z |
| format | Article |
| id | doaj.art-aa47dc8c30f449f8ab2dcee135105f8b |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-24T18:43:26Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-aa47dc8c30f449f8ab2dcee135105f8b2024-03-27T09:39:52ZengWileyAdvanced Science2198-38442024-03-011112n/an/a10.1002/advs.202304561Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro NeuronsCorinna Kaspar0Alexander Ivanenko1Julia Lehrich2Jürgen Klingauf3Wolfram H.P. Pernice4Institute of Physics University of Muenster Heisenbergstr. 11 48149 Muenster GermanyCenter for Soft Nanoscience University of Muenster Busso‐Peuss‐Str. 10 48149 Muenster GermanyCenter for Soft Nanoscience University of Muenster Busso‐Peuss‐Str. 10 48149 Muenster GermanyCenter for Soft Nanoscience University of Muenster Busso‐Peuss‐Str. 10 48149 Muenster GermanyInstitute of Physics University of Muenster Heisenbergstr. 11 48149 Muenster GermanyAbstract Targeted manipulation of neural activity via light has become an indispensable tool for gaining insights into the intricate processes governing single neurons and complex neural networks. To shed light onto the underlying interaction mechanisms, it is crucial to achieve precise control of individual neural activity, as well as a spatial read‐out resolution on the nanoscale. Here, a versatile photonic platform with subcellular resolution for stimulation and monitoring of in‐vitro neurons is demonstrated. Low‐loss photonic waveguides are fabricated on glass substrates using nanoimprint lithography and featuring a loss of only ‐0.9 ± 0.2 dB cm−1 at 489 nm and are combined with optical fiber‐based waveguide‐access and backside total internal reflection fluorescence microscopy. Neurons are grown on the bio‐functionalized photonic chip surface and, expressing the light‐sensitive ion channel Channelrhodopsin‐2, are stimulated within the evanescent field penetration depth of 57 nm of the biocompatible waveguides. The versatility and cost‐efficiency of the platform, along with the possible subcellular resolution, enable tailor‐made investigations of neural interaction dynamics with defined spatial control and high throughput.https://doi.org/10.1002/advs.202304561biohybrid devicescultured neuronsNanophotonic circuitsoptical stimulation |
| spellingShingle | Corinna Kaspar Alexander Ivanenko Julia Lehrich Jürgen Klingauf Wolfram H.P. Pernice Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons Advanced Science biohybrid devices cultured neurons Nanophotonic circuits optical stimulation |
| title | Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons |
| title_full | Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons |
| title_fullStr | Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons |
| title_full_unstemmed | Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons |
| title_short | Biohybrid Photonic Platform for Subcellular Stimulation and Readout of In Vitro Neurons |
| title_sort | biohybrid photonic platform for subcellular stimulation and readout of in vitro neurons |
| topic | biohybrid devices cultured neurons Nanophotonic circuits optical stimulation |
| url | https://doi.org/10.1002/advs.202304561 |
| work_keys_str_mv | AT corinnakaspar biohybridphotonicplatformforsubcellularstimulationandreadoutofinvitroneurons AT alexanderivanenko biohybridphotonicplatformforsubcellularstimulationandreadoutofinvitroneurons AT julialehrich biohybridphotonicplatformforsubcellularstimulationandreadoutofinvitroneurons AT jurgenklingauf biohybridphotonicplatformforsubcellularstimulationandreadoutofinvitroneurons AT wolframhppernice biohybridphotonicplatformforsubcellularstimulationandreadoutofinvitroneurons |