Insights into how environment shapes post-mortem RNA transcription in mouse brain
Abstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-92268-y |
| _version_ | 1818736692813627392 |
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| author | Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira |
| author_facet | Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira |
| author_sort | Raphael Severino Bonadio |
| collection | DOAJ |
| description | Abstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem condition, but little is known about how the environment could influence RNA degradation and transcriptional regulation. In this work, we analysed the transcriptome of mouse brain after death under three concealment simulations (air exposed, buried, and submerged). Our analyses identified 2,103 genes differentially expressed in all tested groups 48 h after death. Moreover, we identified 111 commonly upregulated and 497 commonly downregulated genes in mice from the concealment simulations. The gene functions shared by the individuals from the tested environments were associated with RNA homeostasis, inflammation, developmental processes, cell communication, cell proliferation, and lipid metabolism. Regarding the altered biological processes, we identified that the macroautophagy process was enriched in the upregulated genes and lipid metabolism was enriched in the downregulated genes. On the other hand, we also described a list of biomarkers associated with the submerged and buried groups, indicating that these environments can influence the post-mortem RNA abundance in its particular way. |
| first_indexed | 2024-12-18T00:41:12Z |
| format | Article |
| id | doaj.art-aa4a2f0180414d109838050b6d288e78 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T00:41:12Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-aa4a2f0180414d109838050b6d288e782022-12-21T21:26:52ZengNature PortfolioScientific Reports2045-23222021-06-011111910.1038/s41598-021-92268-yInsights into how environment shapes post-mortem RNA transcription in mouse brainRaphael Severino Bonadio0Larissa Barbosa Nunes1Patricia Natália S. Moretti2Juliana Forte Mazzeu3Stefano Cagnin4Aline Pic-Taylor5Silviene Fabiana de Oliveira6Department of Genetics and Morphology, University of BrasiliaDepartment of Genetics and Morphology, University of BrasiliaFaculty of Medicine, University of BrasiliaFaculty of Medicine, University of BrasiliaDepartment of Biology and CRIBI Biotechnology Centre, University of PadovaDepartment of Genetics and Morphology, University of BrasiliaDepartment of Genetics and Morphology, University of BrasiliaAbstract Most biological features that occur on the body after death were already deciphered by traditional medicine. However, the molecular mechanisms triggered in the cellular microenvironment are not fully comprehended yet. Previous studies reported gene expression alterations in the post-mortem condition, but little is known about how the environment could influence RNA degradation and transcriptional regulation. In this work, we analysed the transcriptome of mouse brain after death under three concealment simulations (air exposed, buried, and submerged). Our analyses identified 2,103 genes differentially expressed in all tested groups 48 h after death. Moreover, we identified 111 commonly upregulated and 497 commonly downregulated genes in mice from the concealment simulations. The gene functions shared by the individuals from the tested environments were associated with RNA homeostasis, inflammation, developmental processes, cell communication, cell proliferation, and lipid metabolism. Regarding the altered biological processes, we identified that the macroautophagy process was enriched in the upregulated genes and lipid metabolism was enriched in the downregulated genes. On the other hand, we also described a list of biomarkers associated with the submerged and buried groups, indicating that these environments can influence the post-mortem RNA abundance in its particular way.https://doi.org/10.1038/s41598-021-92268-y |
| spellingShingle | Raphael Severino Bonadio Larissa Barbosa Nunes Patricia Natália S. Moretti Juliana Forte Mazzeu Stefano Cagnin Aline Pic-Taylor Silviene Fabiana de Oliveira Insights into how environment shapes post-mortem RNA transcription in mouse brain Scientific Reports |
| title | Insights into how environment shapes post-mortem RNA transcription in mouse brain |
| title_full | Insights into how environment shapes post-mortem RNA transcription in mouse brain |
| title_fullStr | Insights into how environment shapes post-mortem RNA transcription in mouse brain |
| title_full_unstemmed | Insights into how environment shapes post-mortem RNA transcription in mouse brain |
| title_short | Insights into how environment shapes post-mortem RNA transcription in mouse brain |
| title_sort | insights into how environment shapes post mortem rna transcription in mouse brain |
| url | https://doi.org/10.1038/s41598-021-92268-y |
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