Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos
Parthenogenesis is an activation process of oocytes that occur without the participation of sperm. Evidence suggests that normal development of embryos requires proper expression of several imprinted genes inherited from both the paternal and maternal genomes. Compared to gene expression, histone mo...
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2018-12-01
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| Series: | The Journal of Reproduction and Development |
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| Online Access: | https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-028/_pdf/-char/en |
| _version_ | 1797614679781015552 |
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| author | Ngan Thi Kim HO Thuy Van Thi NGUYEN Thuan Van NGUYEN Hong-Thuy BUI |
| author_facet | Ngan Thi Kim HO Thuy Van Thi NGUYEN Thuan Van NGUYEN Hong-Thuy BUI |
| author_sort | Ngan Thi Kim HO |
| collection | DOAJ |
| description | Parthenogenesis is an activation process of oocytes that occur without the participation of sperm. Evidence suggests that normal development of embryos requires proper expression of several imprinted genes inherited from both the paternal and maternal genomes. Compared to gene expression, histone modifications and chromatin remodeling are not well-documented. In this research, by using immunofluorescence staining for several developmental-associated histone modifications, we investigated whether epigenetic impairments in parthenogenetic embryos act as constraints for proper development. At early stages, fertilized embryos exhibited high methylation of histone H3 at lysine 9 (Me-H3-K9) and Heterochromatin Protein 1 (HP1) present in the maternal chromatin, while paternal chromatin showed weaker HP1 signals. We found that at the two-cell stage in fertilized embryos, HP1, initially detected around the nucleolus, colocalized with chromocenters at one pole of the blastomere, while parthenotes showed a diffused distribution pattern of HP1 throughout the entire nucleoplasm. At the four-cell stage, methylation of histone H3 at arginine 26 (Me-H3-R26) increased at nascent RNA repression sites in fertilized embryos, while parthenotes recorded weaker signals throughout the nucleoplasm, suggesting differences in pluripotency of the ICM cells between the two types of embryos. Moreover, at the blastocyst stage, we observed that the acetylation level of histone H4 at lysine 12 (Ac-H4-K12) was significantly decreased in parthenogenetic ICM compared to that in its fertilized counterpart. To summarize, differences in epigenetic modifications correlating with paternal chromatin’s capacity to regulate nascent RNA repression may contribute to aberrant development and lineage allocation in mouse parthenogenetic embryos. |
| first_indexed | 2024-03-11T07:15:45Z |
| format | Article |
| id | doaj.art-aa4a897dd39e4745b0f35d659f322bc5 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:15:45Z |
| publishDate | 2018-12-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-aa4a897dd39e4745b0f35d659f322bc52023-11-17T08:17:57ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002018-12-01651839010.1262/jrd.2018-028jrdEpigenetic impairments in development of parthenogenetic preimplantation mouse embryosNgan Thi Kim HO0Thuy Van Thi NGUYEN1Thuan Van NGUYEN2Hong-Thuy BUI3Cellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, VietnamCellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, VietnamCellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, VietnamCellular Reprogramming Laboratory, School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City 700000, VietnamParthenogenesis is an activation process of oocytes that occur without the participation of sperm. Evidence suggests that normal development of embryos requires proper expression of several imprinted genes inherited from both the paternal and maternal genomes. Compared to gene expression, histone modifications and chromatin remodeling are not well-documented. In this research, by using immunofluorescence staining for several developmental-associated histone modifications, we investigated whether epigenetic impairments in parthenogenetic embryos act as constraints for proper development. At early stages, fertilized embryos exhibited high methylation of histone H3 at lysine 9 (Me-H3-K9) and Heterochromatin Protein 1 (HP1) present in the maternal chromatin, while paternal chromatin showed weaker HP1 signals. We found that at the two-cell stage in fertilized embryos, HP1, initially detected around the nucleolus, colocalized with chromocenters at one pole of the blastomere, while parthenotes showed a diffused distribution pattern of HP1 throughout the entire nucleoplasm. At the four-cell stage, methylation of histone H3 at arginine 26 (Me-H3-R26) increased at nascent RNA repression sites in fertilized embryos, while parthenotes recorded weaker signals throughout the nucleoplasm, suggesting differences in pluripotency of the ICM cells between the two types of embryos. Moreover, at the blastocyst stage, we observed that the acetylation level of histone H4 at lysine 12 (Ac-H4-K12) was significantly decreased in parthenogenetic ICM compared to that in its fertilized counterpart. To summarize, differences in epigenetic modifications correlating with paternal chromatin’s capacity to regulate nascent RNA repression may contribute to aberrant development and lineage allocation in mouse parthenogenetic embryos.https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-028/_pdf/-char/enepigeneticshistone acetylationhistone methylationmouse parthenogenetic embryonascent rna production |
| spellingShingle | Ngan Thi Kim HO Thuy Van Thi NGUYEN Thuan Van NGUYEN Hong-Thuy BUI Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos The Journal of Reproduction and Development epigenetics histone acetylation histone methylation mouse parthenogenetic embryo nascent rna production |
| title | Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| title_full | Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| title_fullStr | Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| title_full_unstemmed | Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| title_short | Epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| title_sort | epigenetic impairments in development of parthenogenetic preimplantation mouse embryos |
| topic | epigenetics histone acetylation histone methylation mouse parthenogenetic embryo nascent rna production |
| url | https://www.jstage.jst.go.jp/article/jrd/65/1/65_2018-028/_pdf/-char/en |
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