Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA
Neuropeptides play an important role in modulating mesolimbic system function. However, while synaptic inputs to the ventral tegmental area (VTA) have been extensively mapped, the sources of many neuropeptides are not well resolved. Here, we mapped the anatomical locations of three neuropeptide inpu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Neural Circuits |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncir.2022.918839/full |
| _version_ | 1818117764606328832 |
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| author | Marta E. Soden Joshua X. Yee Beatriz Cuevas Ariana Rastani Jordan Elum Larry S. Zweifel Larry S. Zweifel |
| author_facet | Marta E. Soden Joshua X. Yee Beatriz Cuevas Ariana Rastani Jordan Elum Larry S. Zweifel Larry S. Zweifel |
| author_sort | Marta E. Soden |
| collection | DOAJ |
| description | Neuropeptides play an important role in modulating mesolimbic system function. However, while synaptic inputs to the ventral tegmental area (VTA) have been extensively mapped, the sources of many neuropeptides are not well resolved. Here, we mapped the anatomical locations of three neuropeptide inputs to the VTA: neurotensin (NTS), corticotrophin releasing factor (CRF), and neurokinin B (NkB). Among numerous labeled inputs we identified the bed nucleus of the stria terminalis (BNST) as a major source of all three peptides, containing similar numbers of NTS, CRF, and NkB VTA projection neurons. Approximately 50% of BNST to VTA inputs co-expressed two or more of the peptides examined. Consistent with this expression pattern, analysis of calcium dynamics in the terminals of these inputs in the VTA revealed both common and distinct patterns of activation during appetitive and aversive conditioning. These data demonstrate additional diversification of the mesolimbic dopamine system through partially overlapping neuropeptidergic inputs. |
| first_indexed | 2024-12-11T04:43:36Z |
| format | Article |
| id | doaj.art-aa4d23b01d6148d4bec9a628cfbd5fbf |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-12-11T04:43:36Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-aa4d23b01d6148d4bec9a628cfbd5fbf2022-12-22T01:20:33ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102022-07-011610.3389/fncir.2022.918839918839Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTAMarta E. Soden0Joshua X. Yee1Beatriz Cuevas2Ariana Rastani3Jordan Elum4Larry S. Zweifel5Larry S. Zweifel6Department of Pharmacology, University of Washington, Seattle, WA, United StatesDepartment of Pharmacology, University of Washington, Seattle, WA, United StatesDepartment of Pharmacology, University of Washington, Seattle, WA, United StatesDepartment of Pharmacology, University of Washington, Seattle, WA, United StatesDepartment of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA, United StatesDepartment of Pharmacology, University of Washington, Seattle, WA, United StatesDepartment of Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA, United StatesNeuropeptides play an important role in modulating mesolimbic system function. However, while synaptic inputs to the ventral tegmental area (VTA) have been extensively mapped, the sources of many neuropeptides are not well resolved. Here, we mapped the anatomical locations of three neuropeptide inputs to the VTA: neurotensin (NTS), corticotrophin releasing factor (CRF), and neurokinin B (NkB). Among numerous labeled inputs we identified the bed nucleus of the stria terminalis (BNST) as a major source of all three peptides, containing similar numbers of NTS, CRF, and NkB VTA projection neurons. Approximately 50% of BNST to VTA inputs co-expressed two or more of the peptides examined. Consistent with this expression pattern, analysis of calcium dynamics in the terminals of these inputs in the VTA revealed both common and distinct patterns of activation during appetitive and aversive conditioning. These data demonstrate additional diversification of the mesolimbic dopamine system through partially overlapping neuropeptidergic inputs.https://www.frontiersin.org/articles/10.3389/fncir.2022.918839/fullventral tegmental area (VTA)bed nucleus of the stria terminalis (BNST)NTSCRFNkBTac2 |
| spellingShingle | Marta E. Soden Joshua X. Yee Beatriz Cuevas Ariana Rastani Jordan Elum Larry S. Zweifel Larry S. Zweifel Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA Frontiers in Neural Circuits ventral tegmental area (VTA) bed nucleus of the stria terminalis (BNST) NTS CRF NkB Tac2 |
| title | Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA |
| title_full | Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA |
| title_fullStr | Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA |
| title_full_unstemmed | Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA |
| title_short | Distinct Encoding of Reward and Aversion by Peptidergic BNST Inputs to the VTA |
| title_sort | distinct encoding of reward and aversion by peptidergic bnst inputs to the vta |
| topic | ventral tegmental area (VTA) bed nucleus of the stria terminalis (BNST) NTS CRF NkB Tac2 |
| url | https://www.frontiersin.org/articles/10.3389/fncir.2022.918839/full |
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