Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle)
Studies have found that the substances secreted by the nidamental gland are involved in the formation of the third-level membrane of the fertilized egg and may be closely related to the reproductive activities of cephalopods. However, there is currently a lack in molecular evidence of the involvemen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-04-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000339 |
| _version_ | 1797862129347330048 |
|---|---|
| author | Jinyong Zhang Muchun He Changlin Liu Shufang Liu ZhiMeng Zhuang |
| author_facet | Jinyong Zhang Muchun He Changlin Liu Shufang Liu ZhiMeng Zhuang |
| author_sort | Jinyong Zhang |
| collection | DOAJ |
| description | Studies have found that the substances secreted by the nidamental gland are involved in the formation of the third-level membrane of the fertilized egg and may be closely related to the reproductive activities of cephalopods. However, there is currently a lack in molecular evidence of the involvement of the nidamental gland in reproductive regulation. In this study, RNA sequencing was performed of the nidamental gland tissue of female golden cuttlefish, Sepia esculenta (Hoyle) collected at three key physiological stages: growth stage (NG), spawning stage (NS), and post-reproductive death stage (NA). In total, 65.59 Gb of clean data (Q20 > 96%) were obtained. Pairwise comparative analyses of NG vs. NS and NG vs. NA identified 1889 and 6574 differentially expressed genes (DEGs), respectively, while only 95 DEGs were identified by comparison of NS vs. NA. Venn diagrams of the three developmental groups identified 7021 DEGs. By analyzing genes classified as “reproduction” and “reproductive process” in the GO functional classification, the 14–3–3 protein epsilon-like gene and zinc finger RNA-binding protein-like gene were identified. The significantly enriched pathways included the cell cycle, protein digestion and absorption, p53 signaling pathway, mTOR signaling pathway, PI3K-Akt signaling pathway, and lysosomes. Furthermore, the “mucin type O-glycan biosynthesis” and “steroid biosynthesis” pathways related to reproduction were significantly enriched during spawning and senescent stages. This study clarified the regulation of transcript expression in the nidamental gland of S. esculenta at different physiological stages, thereby providing a valuable genomic resource to reveal the role of the nidamental gland in reproduction. |
| first_indexed | 2024-04-09T22:15:22Z |
| format | Article |
| id | doaj.art-aa4d9d9064de44e89d0f0b651d2009a5 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:15:22Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-aa4d9d9064de44e89d0f0b651d2009a52023-03-23T04:36:22ZengElsevierAquaculture Reports2352-51342023-04-0129101494Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle)Jinyong Zhang0Muchun He1Changlin Liu2Shufang Liu3ZhiMeng Zhuang4Marine Ecology Department, National Marine Environmental Monitoring Center, Dalian 116023, China; Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaMinistry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China; Corresponding author at: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, ChinaStudies have found that the substances secreted by the nidamental gland are involved in the formation of the third-level membrane of the fertilized egg and may be closely related to the reproductive activities of cephalopods. However, there is currently a lack in molecular evidence of the involvement of the nidamental gland in reproductive regulation. In this study, RNA sequencing was performed of the nidamental gland tissue of female golden cuttlefish, Sepia esculenta (Hoyle) collected at three key physiological stages: growth stage (NG), spawning stage (NS), and post-reproductive death stage (NA). In total, 65.59 Gb of clean data (Q20 > 96%) were obtained. Pairwise comparative analyses of NG vs. NS and NG vs. NA identified 1889 and 6574 differentially expressed genes (DEGs), respectively, while only 95 DEGs were identified by comparison of NS vs. NA. Venn diagrams of the three developmental groups identified 7021 DEGs. By analyzing genes classified as “reproduction” and “reproductive process” in the GO functional classification, the 14–3–3 protein epsilon-like gene and zinc finger RNA-binding protein-like gene were identified. The significantly enriched pathways included the cell cycle, protein digestion and absorption, p53 signaling pathway, mTOR signaling pathway, PI3K-Akt signaling pathway, and lysosomes. Furthermore, the “mucin type O-glycan biosynthesis” and “steroid biosynthesis” pathways related to reproduction were significantly enriched during spawning and senescent stages. This study clarified the regulation of transcript expression in the nidamental gland of S. esculenta at different physiological stages, thereby providing a valuable genomic resource to reveal the role of the nidamental gland in reproduction.http://www.sciencedirect.com/science/article/pii/S2352513423000339Differentially expressed genesNidamental glandReproductive regulationSepia esculentaTranscriptome |
| spellingShingle | Jinyong Zhang Muchun He Changlin Liu Shufang Liu ZhiMeng Zhuang Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) Aquaculture Reports Differentially expressed genes Nidamental gland Reproductive regulation Sepia esculenta Transcriptome |
| title | Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) |
| title_full | Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) |
| title_fullStr | Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) |
| title_full_unstemmed | Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) |
| title_short | Transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish (Sepia esculenta Hoyle) |
| title_sort | transcriptome analysis of putative key signaling pathways and genes in the nidamental gland tissue associated with reproductive regulation of golden cuttlefish sepia esculenta hoyle |
| topic | Differentially expressed genes Nidamental gland Reproductive regulation Sepia esculenta Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000339 |
| work_keys_str_mv | AT jinyongzhang transcriptomeanalysisofputativekeysignalingpathwaysandgenesinthenidamentalglandtissueassociatedwithreproductiveregulationofgoldencuttlefishsepiaesculentahoyle AT muchunhe transcriptomeanalysisofputativekeysignalingpathwaysandgenesinthenidamentalglandtissueassociatedwithreproductiveregulationofgoldencuttlefishsepiaesculentahoyle AT changlinliu transcriptomeanalysisofputativekeysignalingpathwaysandgenesinthenidamentalglandtissueassociatedwithreproductiveregulationofgoldencuttlefishsepiaesculentahoyle AT shufangliu transcriptomeanalysisofputativekeysignalingpathwaysandgenesinthenidamentalglandtissueassociatedwithreproductiveregulationofgoldencuttlefishsepiaesculentahoyle AT zhimengzhuang transcriptomeanalysisofputativekeysignalingpathwaysandgenesinthenidamentalglandtissueassociatedwithreproductiveregulationofgoldencuttlefishsepiaesculentahoyle |