Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i>
European fodder turnips (<i>Brassica rapa</i> ssp. <i>rapifera</i>) were identified as sources of clubroot resistance (CR) and have been widely used in <i>Brassica</i> resistance breeding. An F<sub>2</sub> population derived from a cross between a resi...
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MDPI AG
2022-01-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/8/1/66 |
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| author | Hui Zhang Xiaochao Ma Xitong Liu Shifan Zhang Fei Li Guoliang Li Rifei Sun Shujiang Zhang |
| author_facet | Hui Zhang Xiaochao Ma Xitong Liu Shifan Zhang Fei Li Guoliang Li Rifei Sun Shujiang Zhang |
| author_sort | Hui Zhang |
| collection | DOAJ |
| description | European fodder turnips (<i>Brassica rapa</i> ssp. <i>rapifera</i>) were identified as sources of clubroot resistance (CR) and have been widely used in <i>Brassica</i> resistance breeding. An F<sub>2</sub> population derived from a cross between a resistant turnip and a susceptible Chinese cabbage was used to determine the inheritance and locating the resistance Quantitative Trait Loci (QTLs). The parents showed to be very resistant/susceptible to the field isolates (pathotype 4) of clubroot from Henan in China. After inoculation, 27 very resistant or susceptible individuals were selected to construct bulks, respectively. Next-generation-sequencing-based Bulk Segregant Analysis Sequencing (BSA-Seq) was used and located resistance QTL on chromosome A03 (3.3–7.5 Mb) and A08 (0.01–6.5 Mb), named <i>Bcr1</i> and <i>Bcr2</i>, respectively. Furthermore, an F<sub>3</sub> population including 180 families derived from F<sub>2</sub> individuals was phenotyped and used to verify and narrow candidate regions. Ten and seven Kompetitive Allele-Specific PCR (KASP) markers narrowed the target regions to 4.3–4.78 Mb (A03) and 0.02–0.79 Mb (A08), respectively. The phenotypic variation explained (PVE) of the two QTLs were 33.3% and 13.3% respectively. The two candidate regions contained 99 and 109 genes. In the A03 candidate region, there were three candidate R genes, namely <i>Bra006630</i>, <i>Bra006631</i> and <i>Bra006632</i>. In the A08 candidate region, there were two candidate R genes, namely <i>Bra030815</i> and <i>Bra030846</i>. |
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| spelling | doaj.art-aa4dc63559534050bc347a2ef2b5b99d2023-11-23T13:58:03ZengMDPI AGHorticulturae2311-75242022-01-01816610.3390/horticulturae8010066Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i>Hui Zhang0Xiaochao Ma1Xitong Liu2Shifan Zhang3Fei Li4Guoliang Li5Rifei Sun6Shujiang Zhang7Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Zhongguancun, South Street No. 12, Haidian District, Beijing 100081, ChinaEuropean fodder turnips (<i>Brassica rapa</i> ssp. <i>rapifera</i>) were identified as sources of clubroot resistance (CR) and have been widely used in <i>Brassica</i> resistance breeding. An F<sub>2</sub> population derived from a cross between a resistant turnip and a susceptible Chinese cabbage was used to determine the inheritance and locating the resistance Quantitative Trait Loci (QTLs). The parents showed to be very resistant/susceptible to the field isolates (pathotype 4) of clubroot from Henan in China. After inoculation, 27 very resistant or susceptible individuals were selected to construct bulks, respectively. Next-generation-sequencing-based Bulk Segregant Analysis Sequencing (BSA-Seq) was used and located resistance QTL on chromosome A03 (3.3–7.5 Mb) and A08 (0.01–6.5 Mb), named <i>Bcr1</i> and <i>Bcr2</i>, respectively. Furthermore, an F<sub>3</sub> population including 180 families derived from F<sub>2</sub> individuals was phenotyped and used to verify and narrow candidate regions. Ten and seven Kompetitive Allele-Specific PCR (KASP) markers narrowed the target regions to 4.3–4.78 Mb (A03) and 0.02–0.79 Mb (A08), respectively. The phenotypic variation explained (PVE) of the two QTLs were 33.3% and 13.3% respectively. The two candidate regions contained 99 and 109 genes. In the A03 candidate region, there were three candidate R genes, namely <i>Bra006630</i>, <i>Bra006631</i> and <i>Bra006632</i>. In the A08 candidate region, there were two candidate R genes, namely <i>Bra030815</i> and <i>Bra030846</i>.https://www.mdpi.com/2311-7524/8/1/66<i>Plasmodiophora brassicae</i><i>Brassica rapa</i>QTLresistant genesBSA-Seqfine-mapping |
| spellingShingle | Hui Zhang Xiaochao Ma Xitong Liu Shifan Zhang Fei Li Guoliang Li Rifei Sun Shujiang Zhang Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> Horticulturae <i>Plasmodiophora brassicae</i> <i>Brassica rapa</i> QTL resistant genes BSA-Seq fine-mapping |
| title | Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> |
| title_full | Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> |
| title_fullStr | Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> |
| title_full_unstemmed | Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> |
| title_short | Identification and Fine-Mapping of Clubroot (<i>Plasmodiophora</i> <i>brassicae</i>) Resistant QTL in <i>Brassica rapa</i> |
| title_sort | identification and fine mapping of clubroot i plasmodiophora i i brassicae i resistant qtl in i brassica rapa i |
| topic | <i>Plasmodiophora brassicae</i> <i>Brassica rapa</i> QTL resistant genes BSA-Seq fine-mapping |
| url | https://www.mdpi.com/2311-7524/8/1/66 |
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