The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet
Abstract Background The newly proposed methanogenic order ‘Methanomassiliicoccales’ is the second largest archaeal population in the rumen, second only to the Methanobrevibacter population. However, information is limited regarding the community of this new order in the rumen. Methods This study use...
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| Format: | Article |
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BMC
2017-06-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | http://link.springer.com/article/10.1186/s40104-017-0178-0 |
| _version_ | 1818111384223744000 |
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| author | Wei Jin Yanfen Cheng Weiyun Zhu |
| author_facet | Wei Jin Yanfen Cheng Weiyun Zhu |
| author_sort | Wei Jin |
| collection | DOAJ |
| description | Abstract Background The newly proposed methanogenic order ‘Methanomassiliicoccales’ is the second largest archaeal population in the rumen, second only to the Methanobrevibacter population. However, information is limited regarding the community of this new order in the rumen. Methods This study used real-time PCR and 454 pyrosequencing to explore the abundance and community composition of Methanomassiliicoccales in the rumen of Chinese goats fed a hay (0% grain, n = 5) or a high grain (65% grain, n = 5) diet. Results Real-time PCR analysis showed that the relative abundance of Methanomassiliicoccales (% of total archaea) in the goat rumen was significantly lower in the high-grain-diet group (0.5% ± 0.2%) than that in the hay-diet group (8.2% ± 1.1%, P < 0.05). The pyrosequencing results showed that a total of 208 operational taxonomic units (OTUs) were formed from ten samples at 99% sequence identity. All the sequences were identified as Methanomassiliicoccaceae at the family level, and most of the sequences (96.82% ± 1.64%) were further classified as Group 8, 9, and 10 at the Methanomassiliicoccales genus level in each sample based on the RIM-DB database. No significant differences were observed in the number of OTUs or Chao1’s, Shannon’s or Pielou’s evenness indexes between the hay- and high-grain-diet groups (P ≥ 0.05). PCoA analysis showed that diet altered the community of Methanomassiliicoccales. At the genus level, the relative abundances of Group 10 (67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4 (2.07 ± 1.30 vs. 0.27 ± 0.30, P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group 9 was significantly higher in the hay-diet group (18.82 ± 6.20 vs. 47.14 ± 17.72, P = 0.020). At the species level, the relative abundance of Group 10 sp. (67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4 sp. MpT1 (2.07 ± 1.30 vs. 0.27 ± 0.30, P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group 9 sp. ISO4-G1 was significantly higher in the hay-diet group (12.83 ± 3.87 vs. 42.44 ± 18.47, P = 0.022). Conclusions Only a few highly abundant phylogenetic groups dominated within the Methanomassiliicoccales community in the rumens of Chinese goats, and these were easily depressed by high-grain-diet feeding. The relatively low abundance suggests a small contribution on the part of Methanomassiliicoccales to the rumen methanogenesis of Chinese goats. |
| first_indexed | 2024-12-11T03:02:11Z |
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| language | English |
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| spelling | doaj.art-aa4e070acc3c43899fd5eab64de52e8c2022-12-22T01:23:02ZengBMCJournal of Animal Science and Biotechnology2049-18912017-06-018111010.1186/s40104-017-0178-0The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain dietWei Jin0Yanfen Cheng1Weiyun Zhu2Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural UniversityJiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural UniversityJiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health; Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural UniversityAbstract Background The newly proposed methanogenic order ‘Methanomassiliicoccales’ is the second largest archaeal population in the rumen, second only to the Methanobrevibacter population. However, information is limited regarding the community of this new order in the rumen. Methods This study used real-time PCR and 454 pyrosequencing to explore the abundance and community composition of Methanomassiliicoccales in the rumen of Chinese goats fed a hay (0% grain, n = 5) or a high grain (65% grain, n = 5) diet. Results Real-time PCR analysis showed that the relative abundance of Methanomassiliicoccales (% of total archaea) in the goat rumen was significantly lower in the high-grain-diet group (0.5% ± 0.2%) than that in the hay-diet group (8.2% ± 1.1%, P < 0.05). The pyrosequencing results showed that a total of 208 operational taxonomic units (OTUs) were formed from ten samples at 99% sequence identity. All the sequences were identified as Methanomassiliicoccaceae at the family level, and most of the sequences (96.82% ± 1.64%) were further classified as Group 8, 9, and 10 at the Methanomassiliicoccales genus level in each sample based on the RIM-DB database. No significant differences were observed in the number of OTUs or Chao1’s, Shannon’s or Pielou’s evenness indexes between the hay- and high-grain-diet groups (P ≥ 0.05). PCoA analysis showed that diet altered the community of Methanomassiliicoccales. At the genus level, the relative abundances of Group 10 (67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4 (2.07 ± 1.30 vs. 0.27 ± 0.30, P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group 9 was significantly higher in the hay-diet group (18.82 ± 6.20 vs. 47.14 ± 17.72, P = 0.020). At the species level, the relative abundance of Group 10 sp. (67.25 ± 12.76 vs. 38.13 ± 15.66, P = 0.012) and Group 4 sp. MpT1 (2.07 ± 1.30 vs. 0.27 ± 0.30, P = 0.035) were significantly higher in the high-grain-diet group, while the relative abundance of Group 9 sp. ISO4-G1 was significantly higher in the hay-diet group (12.83 ± 3.87 vs. 42.44 ± 18.47, P = 0.022). Conclusions Only a few highly abundant phylogenetic groups dominated within the Methanomassiliicoccales community in the rumens of Chinese goats, and these were easily depressed by high-grain-diet feeding. The relatively low abundance suggests a small contribution on the part of Methanomassiliicoccales to the rumen methanogenesis of Chinese goats.http://link.springer.com/article/10.1186/s40104-017-0178-0Community structureDietGoatMethanomassiliicoccalesPyrosequencing |
| spellingShingle | Wei Jin Yanfen Cheng Weiyun Zhu The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet Journal of Animal Science and Biotechnology Community structure Diet Goat Methanomassiliicoccales Pyrosequencing |
| title | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet |
| title_full | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet |
| title_fullStr | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet |
| title_full_unstemmed | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet |
| title_short | The community structure of Methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet |
| title_sort | community structure of methanomassiliicoccales in the rumen of chinese goats and its response to a high grain diet |
| topic | Community structure Diet Goat Methanomassiliicoccales Pyrosequencing |
| url | http://link.springer.com/article/10.1186/s40104-017-0178-0 |
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