Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats
The use of hematological leukocytes ratio indices (HLRI), which show the interrelations between different blood cells, for evaluation the organism state of laboratory rats is questionable. The aim is to investigate the informativity of HLRI in laboratory rats on the model of acute infectious process...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2021-08-01
|
| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=40291 |
| _version_ | 1818937531398356992 |
|---|---|
| author | Raisa Oleksandrivna Lytvynenko Lyudmyla Valeriyivna Makyeyeva |
| author_facet | Raisa Oleksandrivna Lytvynenko Lyudmyla Valeriyivna Makyeyeva |
| author_sort | Raisa Oleksandrivna Lytvynenko |
| collection | DOAJ |
| description | The use of hematological leukocytes ratio indices (HLRI), which show the interrelations between different blood cells, for evaluation the organism state of laboratory rats is questionable. The aim is to investigate the informativity of HLRI in laboratory rats on the model of acute infectious process. The study performed on white nonlinear (78 months) male laboratory rats: control (n = 10) and experimental groups (n = 10), which simulated acute purulent fecal peritonitis. Analyzed the number of leukocytes, blood leukocyte formula, phagocytic activity of neutrophils, HLRI. Acute infectious process is accompanied by leukocytosis, neutrophilia with leukocyte blood formula shift to the left and inhibition of neutrophils absorption capacity. A set of changes of HLRI in experimental group animals reflects the predominance of cellular immunity over the humoral, prevalence of granulocytes and their immature forms, increased activity of the inflammatory process, predominance of the microphage system, intense non-specific immunity, disturbance of immune system functional state with a shift of balance towards monokines, formation of mainly delayed-type hypersensitivity reactions, decreased function of affector cells and predominance of effector part of immunological process, the infectious nature of intoxication. Analysis with ROC curves displayed that 8 from 16 applied HLRI, such as blood leukocyte shift index, neutrophil to lymphocyte, segmented neutrophil to lymphocyte, banded neutrophil to lymphocyte, eosinophil to lymphocyte, monocyte to lymphocyte, neutrophil to monocyte and segmented neutrophil to monocyte count ratio indices have very high levels of sensitivity and specificity and can be used as diagnostic markers for acute purulent fecal peritonitis. [ J Adv Biotechnol Exp Ther 2021; 4(2.000): 120-132] |
| first_indexed | 2024-12-20T05:53:26Z |
| format | Article |
| id | doaj.art-aa56794c696a4dfda0eb768cb5967967 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-20T05:53:26Z |
| publishDate | 2021-08-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-aa56794c696a4dfda0eb768cb59679672022-12-21T19:51:07ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602021-08-014212013210.5455/jabet.2021.d11340291Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory ratsRaisa Oleksandrivna Lytvynenko0Lyudmyla Valeriyivna Makyeyeva1Department of Physiology, Immunology and Biochemistry with the Course of Civil Defense and Medicine, Faculty of Biology, Zaporizhzhia National University, Zaporizhzhia, 69600, UkraineDepartment of Histology, Cytology and Embryology, Medical Faculty No. 2, Zaporizhzhia State Medical University, Zaporizhzhia, 69035, UkraineThe use of hematological leukocytes ratio indices (HLRI), which show the interrelations between different blood cells, for evaluation the organism state of laboratory rats is questionable. The aim is to investigate the informativity of HLRI in laboratory rats on the model of acute infectious process. The study performed on white nonlinear (78 months) male laboratory rats: control (n = 10) and experimental groups (n = 10), which simulated acute purulent fecal peritonitis. Analyzed the number of leukocytes, blood leukocyte formula, phagocytic activity of neutrophils, HLRI. Acute infectious process is accompanied by leukocytosis, neutrophilia with leukocyte blood formula shift to the left and inhibition of neutrophils absorption capacity. A set of changes of HLRI in experimental group animals reflects the predominance of cellular immunity over the humoral, prevalence of granulocytes and their immature forms, increased activity of the inflammatory process, predominance of the microphage system, intense non-specific immunity, disturbance of immune system functional state with a shift of balance towards monokines, formation of mainly delayed-type hypersensitivity reactions, decreased function of affector cells and predominance of effector part of immunological process, the infectious nature of intoxication. Analysis with ROC curves displayed that 8 from 16 applied HLRI, such as blood leukocyte shift index, neutrophil to lymphocyte, segmented neutrophil to lymphocyte, banded neutrophil to lymphocyte, eosinophil to lymphocyte, monocyte to lymphocyte, neutrophil to monocyte and segmented neutrophil to monocyte count ratio indices have very high levels of sensitivity and specificity and can be used as diagnostic markers for acute purulent fecal peritonitis. [ J Adv Biotechnol Exp Ther 2021; 4(2.000): 120-132]http://www.ejmanager.com/fulltextpdf.php?mno=40291acute purulent fecal peritonitishematological leukocytes ratio indicesleukocytesleukocyte blood formulaphagocytosis |
| spellingShingle | Raisa Oleksandrivna Lytvynenko Lyudmyla Valeriyivna Makyeyeva Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats Journal of Advanced Biotechnology and Experimental Therapeutics acute purulent fecal peritonitis hematological leukocytes ratio indices leukocytes leukocyte blood formula phagocytosis |
| title | Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| title_full | Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| title_fullStr | Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| title_full_unstemmed | Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| title_short | Hematological leukocytes ratio indices: predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| title_sort | hematological leukocytes ratio indices predictors of acute purulent fecal peritonitis in nonlinear laboratory rats |
| topic | acute purulent fecal peritonitis hematological leukocytes ratio indices leukocytes leukocyte blood formula phagocytosis |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=40291 |
| work_keys_str_mv | AT raisaoleksandrivnalytvynenko hematologicalleukocytesratioindicespredictorsofacutepurulentfecalperitonitisinnonlinearlaboratoryrats AT lyudmylavaleriyivnamakyeyeva hematologicalleukocytesratioindicespredictorsofacutepurulentfecalperitonitisinnonlinearlaboratoryrats |