Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood
Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Ad...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-06-01
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| Series: | Molecular Therapy: Methods & Clinical Development |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2329050117300414 |
| _version_ | 1818913754740424704 |
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| author | Hema Dave Min Luo J.W. Blaney Shabnum Patel Cecilia Barese Conrad Russell Cruz Elizabeth J. Shpall Catherine M. Bollard Patrick J. Hanley |
| author_facet | Hema Dave Min Luo J.W. Blaney Shabnum Patel Cecilia Barese Conrad Russell Cruz Elizabeth J. Shpall Catherine M. Bollard Patrick J. Hanley |
| author_sort | Hema Dave |
| collection | DOAJ |
| description | Umbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation. |
| first_indexed | 2024-12-19T23:35:31Z |
| format | Article |
| id | doaj.art-aa567dccad5b4707904daf27e8d527ff |
| institution | Directory Open Access Journal |
| issn | 2329-0501 |
| language | English |
| last_indexed | 2024-12-19T23:35:31Z |
| publishDate | 2017-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Methods & Clinical Development |
| spelling | doaj.art-aa567dccad5b4707904daf27e8d527ff2022-12-21T20:01:37ZengElsevierMolecular Therapy: Methods & Clinical Development2329-05012017-06-015C132110.1016/j.omtm.2017.02.001Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord BloodHema Dave0Min Luo1J.W. Blaney2Shabnum Patel3Cecilia Barese4Conrad Russell Cruz5Elizabeth J. Shpall6Catherine M. Bollard7Patrick J. Hanley8Center for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Texas Children’s Hospital, and Houston Methodist Hospital, Houston, TX 77030, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USADepartment of Stem Cell Transplantation and Cellular Therapy, MD Anderson Cancer Center, Houston, TX 77030, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USACenter for Cancer and Immunology Research, Children’s National Medical Center, Washington, DC 20010, USAUmbilical cord blood (CB) has emerged as an effective alternative donor source for hematopoietic stem cell transplantation. Despite this success, the prolonged duration of immune suppression following CB transplantation and the naiveté of CB T cells leave patients susceptible to viral infections. Adoptive transfer of ex vivo-expanded virus-specific T cells from CB is both feasible and safe. However, the manufacturing process of these cells is complicated, lengthy, and labor-intensive. We have now developed a simplified method to manufacture a single culture of polyclonal multivirus-specific cytotoxic T cells in less than 30 days. It eliminates the need for a live virus or transduction with a viral vector, thus making this approach widely available and GMP-applicable to target multiple viruses. The use of overlapping PepMixes as a source of antigen stimulation enable expansion of the repertoire of the T cell product to any virus of interest and make it available as a third party “off the shelf” treatment for viral infections following transplantation.http://www.sciencedirect.com/science/article/pii/S2329050117300414cord bloodT cellsadoptive immunotherapycellular therapyantiviral T cellsviruscord blood transplantation |
| spellingShingle | Hema Dave Min Luo J.W. Blaney Shabnum Patel Cecilia Barese Conrad Russell Cruz Elizabeth J. Shpall Catherine M. Bollard Patrick J. Hanley Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood Molecular Therapy: Methods & Clinical Development cord blood T cells adoptive immunotherapy cellular therapy antiviral T cells virus cord blood transplantation |
| title | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
| title_full | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
| title_fullStr | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
| title_full_unstemmed | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
| title_short | Toward a Rapid Production of Multivirus-Specific T Cells Targeting BKV, Adenovirus, CMV, and EBV from Umbilical Cord Blood |
| title_sort | toward a rapid production of multivirus specific t cells targeting bkv adenovirus cmv and ebv from umbilical cord blood |
| topic | cord blood T cells adoptive immunotherapy cellular therapy antiviral T cells virus cord blood transplantation |
| url | http://www.sciencedirect.com/science/article/pii/S2329050117300414 |
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