Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization
Abstract The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected ac...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2021-03-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100801&tlng=en |
| _version_ | 1818945773733150720 |
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| author | Vanessa Rodrigues dos SANTOS Remberto Marcelo Argandoña VALDEZ Marcelle DANELON José Antonio Santos SOUZA Karina Sampaio CAIAFFA Alberto Carlos Botazzo DELBEM Cristiane DUQUE |
| author_facet | Vanessa Rodrigues dos SANTOS Remberto Marcelo Argandoña VALDEZ Marcelle DANELON José Antonio Santos SOUZA Karina Sampaio CAIAFFA Alberto Carlos Botazzo DELBEM Cristiane DUQUE |
| author_sort | Vanessa Rodrigues dos SANTOS |
| collection | DOAJ |
| description | Abstract The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (ΔKHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest ∆KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and ∆KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression. |
| first_indexed | 2024-12-20T08:04:27Z |
| format | Article |
| id | doaj.art-aa581e09f84f4f03940f5ad55d712d52 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-20T08:04:27Z |
| publishDate | 2021-03-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-aa581e09f84f4f03940f5ad55d712d522022-12-21T19:47:26ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072021-03-013510.1590/1807-3107bor-2021.vol35.0030Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralizationVanessa Rodrigues dos SANTOShttps://orcid.org/0000-0001-8725-2136Remberto Marcelo Argandoña VALDEZhttps://orcid.org/0000-0003-2329-3873Marcelle DANELONhttps://orcid.org/0000-0003-2091-649XJosé Antonio Santos SOUZAhttps://orcid.org/0000-0001-8606-8257Karina Sampaio CAIAFFAhttps://orcid.org/0000-0002-8159-727XAlberto Carlos Botazzo DELBEMhttps://orcid.org/0000-0002-8159-4853Cristiane DUQUEhttps://orcid.org/0000-0002-2575-279XAbstract The present study evaluated the ability of Bifidobacterium and Lactobacillus species associated with streptococci to increase insoluble extracellular polysaccharide (EPS) production and initial caries lesion progression. Bovine enamel blocks (n = 190; 4 mm x 4 mm) were prepared, selected according to initial surface hardness (SH), and divided into two groups: a) double combinations: S. mutans with Bifidobacterium or Lactobacillus, and b) triple combinations: S. mutans and S. sobrinus with Bifidobacterium or Lactobacillus species. The blocks were exposed to the bacterial associations for 7 days. Subsequently, quantity of EPS from biofilms and caries lesion depth were determined by means of colorimetric and cross-sectional enamel hardness (ΔKHN) analysis. The data were submitted to one-way analysis of variance, followed by the Bonferroni test (p < 0.05). S. mutans with B. animalis or B. dentium produced a higher quantity of EPS; S. mutans + B. animalis led to the highest ∆KHN. S. mutans + S. sobrinus + B. longum induced greater EPS and ∆KHN values. In conclusion, associations of B. animalis and B. longum with streptococci promoted EPS production and caries lesion progression.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100801&tlng=enBifidobacteriumLactobacillusStreptococcusPolysaccharidesDental Caries |
| spellingShingle | Vanessa Rodrigues dos SANTOS Remberto Marcelo Argandoña VALDEZ Marcelle DANELON José Antonio Santos SOUZA Karina Sampaio CAIAFFA Alberto Carlos Botazzo DELBEM Cristiane DUQUE Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization Brazilian Oral Research Bifidobacterium Lactobacillus Streptococcus Polysaccharides Dental Caries |
| title | Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization |
| title_full | Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization |
| title_fullStr | Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization |
| title_full_unstemmed | Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization |
| title_short | Effect of S. mutans combinations with bifidobacteria/lactobacilli on biofilm and enamel demineralization |
| title_sort | effect of s mutans combinations with bifidobacteria lactobacilli on biofilm and enamel demineralization |
| topic | Bifidobacterium Lactobacillus Streptococcus Polysaccharides Dental Caries |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242021000100801&tlng=en |
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