Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic
There is a serious problem with soil salinization that affects the growth and development of plants. <i>Tamarix ramosissima</i> Ledeb (<i>T</i>. <i>ramosissima</i>), as a halophyte, is widely used for afforestation in salinized soils. At present, there are few rep...
| 主要な著者: | , , , , , , |
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| フォーマット: | 論文 |
| 言語: | English |
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MDPI AG
2022-11-01
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| シリーズ: | Antioxidants |
| 主題: | |
| オンライン･アクセス: | https://www.mdpi.com/2076-3921/11/12/2362 |
| _version_ | 1827642131894763520 |
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| author | Yahui Chen Haijia Li Shiyang Zhang Shanfeng Du Guangyu Wang Jinchi Zhang Jiang Jiang |
| author_facet | Yahui Chen Haijia Li Shiyang Zhang Shanfeng Du Guangyu Wang Jinchi Zhang Jiang Jiang |
| author_sort | Yahui Chen |
| collection | DOAJ |
| description | There is a serious problem with soil salinization that affects the growth and development of plants. <i>Tamarix ramosissima</i> Ledeb (<i>T</i>. <i>ramosissima</i>), as a halophyte, is widely used for afforestation in salinized soils. At present, there are few reports on the antioxidant mechanism of <i>T</i>. <i>ramosissima</i> under NaCl stress. In this study, we learned about the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) content changes in <i>T. ramosissima</i>. We also mined the relevant metabolic pathways in the antioxidant mechanism, candidate key genes, and their related differential metabolites and verified them using quantitative real-time PCR (qRT-PCR). The results show that the SOD, POD, and CAT activities, and the H<sub>2</sub>O<sub>2</sub> and MDA content reached the highest values in the roots of <i>T. ramosissima</i>. Simultaneously, 92 differentially expressed genes (DEGs) related to antioxidant enzyme activities changed during 48 and 168 h of NaCl stress, and these DEGs were mainly upregulated in 168 h. Based on the association analysis of transcriptomic and metabolomic data, we found <i>Unigene0089358</i> and <i>Unigene0007782</i> as genes related to key enzymes in the flavonoid biosynthesis pathway. They were located in the upstream positive regulation at 48 and 168 h under NaCl stress, and their respective related metabolites (phloretin and pinocembrin) were involved in resistance to NaCl stress, and they were significantly correlated with their respective metabolites. In conclusion, at 48 and 168 h under NaCl stress, the roots of <i>T. ramosissima</i> resist NaCl stress by enhancing enzymatic and nonenzymatic antioxidant mechanisms, scavenging ROS generated by high-salt stress, alleviating NaCl toxicity, and maintaining the growth of <i>T. ramosissima</i>. This study provides genetic resources and a scientific theoretical basis for further breeding of salt-tolerant <i>Tamarix</i> plants and the molecular mechanism of antioxidants to alleviate NaCl toxicity. |
| first_indexed | 2024-03-09T17:24:17Z |
| format | Article |
| id | doaj.art-aa5b09b1e22446f9bb0089c2769d3cd7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T17:24:17Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-aa5b09b1e22446f9bb0089c2769d3cd72023-11-24T12:56:45ZengMDPI AGAntioxidants2076-39212022-11-011112236210.3390/antiox11122362Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and MetabolomicYahui Chen0Haijia Li1Shiyang Zhang2Shanfeng Du3Guangyu Wang4Jinchi Zhang5Jiang Jiang6Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Forest Resources Management and Faculty of Science, University of British Columbia, Vancouver, BC V6T 1Z4, CanadaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, ChinaDepartment of Forest Resources Management and Faculty of Science, University of British Columbia, Vancouver, BC V6T 1Z4, CanadaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, ChinaCollaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, Nanjing 210037, ChinaThere is a serious problem with soil salinization that affects the growth and development of plants. <i>Tamarix ramosissima</i> Ledeb (<i>T</i>. <i>ramosissima</i>), as a halophyte, is widely used for afforestation in salinized soils. At present, there are few reports on the antioxidant mechanism of <i>T</i>. <i>ramosissima</i> under NaCl stress. In this study, we learned about the superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) content changes in <i>T. ramosissima</i>. We also mined the relevant metabolic pathways in the antioxidant mechanism, candidate key genes, and their related differential metabolites and verified them using quantitative real-time PCR (qRT-PCR). The results show that the SOD, POD, and CAT activities, and the H<sub>2</sub>O<sub>2</sub> and MDA content reached the highest values in the roots of <i>T. ramosissima</i>. Simultaneously, 92 differentially expressed genes (DEGs) related to antioxidant enzyme activities changed during 48 and 168 h of NaCl stress, and these DEGs were mainly upregulated in 168 h. Based on the association analysis of transcriptomic and metabolomic data, we found <i>Unigene0089358</i> and <i>Unigene0007782</i> as genes related to key enzymes in the flavonoid biosynthesis pathway. They were located in the upstream positive regulation at 48 and 168 h under NaCl stress, and their respective related metabolites (phloretin and pinocembrin) were involved in resistance to NaCl stress, and they were significantly correlated with their respective metabolites. In conclusion, at 48 and 168 h under NaCl stress, the roots of <i>T. ramosissima</i> resist NaCl stress by enhancing enzymatic and nonenzymatic antioxidant mechanisms, scavenging ROS generated by high-salt stress, alleviating NaCl toxicity, and maintaining the growth of <i>T. ramosissima</i>. This study provides genetic resources and a scientific theoretical basis for further breeding of salt-tolerant <i>Tamarix</i> plants and the molecular mechanism of antioxidants to alleviate NaCl toxicity.https://www.mdpi.com/2076-3921/11/12/2362NaCl stressantioxidant mechanismtranscriptomemetabolomeNaCl toxicity |
| spellingShingle | Yahui Chen Haijia Li Shiyang Zhang Shanfeng Du Guangyu Wang Jinchi Zhang Jiang Jiang Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic Antioxidants NaCl stress antioxidant mechanism transcriptome metabolome NaCl toxicity |
| title | Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic |
| title_full | Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic |
| title_fullStr | Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic |
| title_full_unstemmed | Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic |
| title_short | Analysis of the Antioxidant Mechanism of <i>Tamarix ramosissima</i> Roots under NaCl Stress Based on Physiology, Transcriptomic and Metabolomic |
| title_sort | analysis of the antioxidant mechanism of i tamarix ramosissima i roots under nacl stress based on physiology transcriptomic and metabolomic |
| topic | NaCl stress antioxidant mechanism transcriptome metabolome NaCl toxicity |
| url | https://www.mdpi.com/2076-3921/11/12/2362 |
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