Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields
Maize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremend...
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MDPI AG
2021-09-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/10/687 |
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| author | Vanja Vlajkov Mila Grahovac Dragana Budakov Marta Loc Ivana Pajčin Dragan Milić Tihomir Novaković Jovana Grahovac |
| author_facet | Vanja Vlajkov Mila Grahovac Dragana Budakov Marta Loc Ivana Pajčin Dragan Milić Tihomir Novaković Jovana Grahovac |
| author_sort | Vanja Vlajkov |
| collection | DOAJ |
| description | Maize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremendous economic losses. No aflatoxin contamination of maize samples harvested in 2019 and 2020 in different localities in the Republic of Serbia was detected by the Enzyme-Linked Immunosorbent Assay (ELISA) test and High-Performance Liquid Chromatography (HPLC) method. On the other hand, the Cluster Amplification Patterns (CAP) analyses of the isolated <i>Aspergillus flavus</i> strains from 2019 maize samples confirmed the presence of key biosynthesis genes responsible for aflatoxin production. Artificial inoculation and subsequent HPLC analysis of the inoculated maize samples confirmed the high capacity of the <i>A. flavus</i> strains for aflatoxin production, pointing to a high risk of contamination under favorable conditions. Prevention of aflatoxin contamination is primarily based on <i>A. flavus</i> control, where biocontrol agents play a significant role as sustainable disease management tools. In this study, antagonistic activity screening of the novel strains belonging to the <i>Bacillus</i> genus indicated superior suppression of <i>A. flavus</i> strains by two <i>Bacillus</i> strains isolated from the rhizosphere of <i>Phaseolus vulgaris.</i> |
| first_indexed | 2024-03-10T06:10:41Z |
| format | Article |
| id | doaj.art-aa5bafb50da3469a87d4e83599f20fd7 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T06:10:41Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-aa5bafb50da3469a87d4e83599f20fd72023-11-22T20:12:55ZengMDPI AGToxins2072-66512021-09-01131068710.3390/toxins13100687Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize FieldsVanja Vlajkov0Mila Grahovac1Dragana Budakov2Marta Loc3Ivana Pajčin4Dragan Milić5Tihomir Novaković6Jovana Grahovac7Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21000 Novi Sad, SerbiaFaculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, SerbiaMaize is one of the leading export products in the Republic of Serbia. As a country where economic development depends on agriculture, maize production plays a critical role as a crop of strategic importance. Potential aflatoxin contamination of maize poses a risk to food and feed safety and tremendous economic losses. No aflatoxin contamination of maize samples harvested in 2019 and 2020 in different localities in the Republic of Serbia was detected by the Enzyme-Linked Immunosorbent Assay (ELISA) test and High-Performance Liquid Chromatography (HPLC) method. On the other hand, the Cluster Amplification Patterns (CAP) analyses of the isolated <i>Aspergillus flavus</i> strains from 2019 maize samples confirmed the presence of key biosynthesis genes responsible for aflatoxin production. Artificial inoculation and subsequent HPLC analysis of the inoculated maize samples confirmed the high capacity of the <i>A. flavus</i> strains for aflatoxin production, pointing to a high risk of contamination under favorable conditions. Prevention of aflatoxin contamination is primarily based on <i>A. flavus</i> control, where biocontrol agents play a significant role as sustainable disease management tools. In this study, antagonistic activity screening of the novel strains belonging to the <i>Bacillus</i> genus indicated superior suppression of <i>A. flavus</i> strains by two <i>Bacillus</i> strains isolated from the rhizosphere of <i>Phaseolus vulgaris.</i>https://www.mdpi.com/2072-6651/13/10/687aflatoxin<i>Aspergillus flavus</i>maizebiocontrolBacillusHPLC |
| spellingShingle | Vanja Vlajkov Mila Grahovac Dragana Budakov Marta Loc Ivana Pajčin Dragan Milić Tihomir Novaković Jovana Grahovac Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields Toxins aflatoxin <i>Aspergillus flavus</i> maize biocontrol Bacillus HPLC |
| title | Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields |
| title_full | Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields |
| title_fullStr | Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields |
| title_full_unstemmed | Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields |
| title_short | Distribution, Genetic Diversity and Biocontrol of Aflatoxigenic <i>Aspergillus flavus</i> in Serbian Maize Fields |
| title_sort | distribution genetic diversity and biocontrol of aflatoxigenic i aspergillus flavus i in serbian maize fields |
| topic | aflatoxin <i>Aspergillus flavus</i> maize biocontrol Bacillus HPLC |
| url | https://www.mdpi.com/2072-6651/13/10/687 |
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