Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC)
Abstract The search for compounds with anticancer effects is of paramount importance today due to the high incidence of the disease. The Euphorbiaceae family is known for having compounds with therapeutic properties, one of its genera being Croton. It has several species, which contain compounds alr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2022-09-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100735&tlng=en |
| _version_ | 1811258370895642624 |
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| author | I. Vendruscolo S. R. T. Venturella P. A. Bressiani I. G. Marco C. R. Novello I. V. Almeida V. E. P. Vicentini J. C. P. Mello E. Düsman |
| author_facet | I. Vendruscolo S. R. T. Venturella P. A. Bressiani I. G. Marco C. R. Novello I. V. Almeida V. E. P. Vicentini J. C. P. Mello E. Düsman |
| author_sort | I. Vendruscolo |
| collection | DOAJ |
| description | Abstract The search for compounds with anticancer effects is of paramount importance today due to the high incidence of the disease. The Euphorbiaceae family is known for having compounds with therapeutic properties, one of its genera being Croton. It has several species, which contain compounds already known for their biological activities, presenting anti-inflammatory, antimicrobial and anticancer properties. Thus, the cytotoxicity/antiproliferative activity of semi-purified fractions and compounds isolated from Croton echioides in liver tumor cells of Rattus norvegicus (HTC) was evaluated by the MTT test. The semi-purified fractions showed cytotoxicity at concentrations above 200 µg/mL, at 24, 48 and 72 hours, reaching cell viability of 24.78% [400 µg/mL] at 24 hours, 12.79% [500 µg/mL] at 48 hours and 10.57% [300 µg/mL] at 72 hours. For the isolated compounds, lupeol had a cytotoxic effect in all concentrations (1, 5, 10, 15, 20, 40, 60, 80 and 100 µg/mL) and tested times (24, 48 and 72 hours), reaching minimum viability of 4.37% [100 µg/mL], within 72 hours. The clerodan diterpenes CEH-1 and CEH-4 also showed antiproliferative activity, with minimum viability of 36.19% [100 µg/mL] over 72 hours and 21.33% [100 µg/mL] over 48 hours, respectively. However, the clerodan diterpenes CEH-2 and CEH-3 did not shows a cytotoxic effect for HTC cells. Thus, there is a cytotoxic/antiproliferative potential of C. echioides against tumor cells, with targeted to mitochondrial enzymes, associated with cell proliferation, indicating that this species deserves prominence in the search for new molecules for the treatment of cancer. |
| first_indexed | 2024-04-12T18:12:27Z |
| format | Article |
| id | doaj.art-aa5ca0e4f4d743d5b37d9df564e24e33 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-12T18:12:27Z |
| publishDate | 2022-09-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-aa5ca0e4f4d743d5b37d9df564e24e332022-12-22T03:21:46ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752022-09-018210.1590/1519-6984.264356Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC)I. Vendruscolohttps://orcid.org/0000-0001-7151-1156S. R. T. Venturellahttps://orcid.org/0000-0001-9588-1215P. A. Bressianihttps://orcid.org/0000-0001-7449-4646I. G. Marcohttps://orcid.org/0000-0002-9667-4450C. R. Novellohttps://orcid.org/0000-0002-6833-7000I. V. Almeidahttps://orcid.org/0000-0003-3200-2126V. E. P. Vicentinihttps://orcid.org/0000-0003-1385-0058J. C. P. Mellohttps://orcid.org/0000-0002-7532-3395E. Düsmanhttps://orcid.org/0000-0002-4483-5638Abstract The search for compounds with anticancer effects is of paramount importance today due to the high incidence of the disease. The Euphorbiaceae family is known for having compounds with therapeutic properties, one of its genera being Croton. It has several species, which contain compounds already known for their biological activities, presenting anti-inflammatory, antimicrobial and anticancer properties. Thus, the cytotoxicity/antiproliferative activity of semi-purified fractions and compounds isolated from Croton echioides in liver tumor cells of Rattus norvegicus (HTC) was evaluated by the MTT test. The semi-purified fractions showed cytotoxicity at concentrations above 200 µg/mL, at 24, 48 and 72 hours, reaching cell viability of 24.78% [400 µg/mL] at 24 hours, 12.79% [500 µg/mL] at 48 hours and 10.57% [300 µg/mL] at 72 hours. For the isolated compounds, lupeol had a cytotoxic effect in all concentrations (1, 5, 10, 15, 20, 40, 60, 80 and 100 µg/mL) and tested times (24, 48 and 72 hours), reaching minimum viability of 4.37% [100 µg/mL], within 72 hours. The clerodan diterpenes CEH-1 and CEH-4 also showed antiproliferative activity, with minimum viability of 36.19% [100 µg/mL] over 72 hours and 21.33% [100 µg/mL] over 48 hours, respectively. However, the clerodan diterpenes CEH-2 and CEH-3 did not shows a cytotoxic effect for HTC cells. Thus, there is a cytotoxic/antiproliferative potential of C. echioides against tumor cells, with targeted to mitochondrial enzymes, associated with cell proliferation, indicating that this species deserves prominence in the search for new molecules for the treatment of cancer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100735&tlng=enantiproliferative activityRattus norvegicus tumor cellCroton echioidesclerodan diterpenesMTT assay |
| spellingShingle | I. Vendruscolo S. R. T. Venturella P. A. Bressiani I. G. Marco C. R. Novello I. V. Almeida V. E. P. Vicentini J. C. P. Mello E. Düsman Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) Brazilian Journal of Biology antiproliferative activity Rattus norvegicus tumor cell Croton echioides clerodan diterpenes MTT assay |
| title | Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) |
| title_full | Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) |
| title_fullStr | Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) |
| title_full_unstemmed | Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) |
| title_short | Cytotoxicity of extracts and compounds isolated from Croton echioides in animal tumor cell (HTC) |
| title_sort | cytotoxicity of extracts and compounds isolated from croton echioides in animal tumor cell htc |
| topic | antiproliferative activity Rattus norvegicus tumor cell Croton echioides clerodan diterpenes MTT assay |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100735&tlng=en |
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