Versatile CRISPR/Cas9 Systems for Genome Editing in <i>Ustilago maydis</i>

Versatile CRISPR/Cas9 Systems for Genome Editing in <i>Ustilago maydis</i>

The phytopathogenic smut fungus <i>Ustilago maydis</i> is a versatile model organism to study plant pathology, fungal genetics, and molecular cell biology. Here, we report several strategies to manipulate the genome of <i>U. maydis</i> by the CRISPR/Cas9 technology. These inc...

Full description

Bibliographic Details
Main Authors:	Sarah-Maria Wege, Katharina Gejer, Fabienne Becker, Michael Bölker, Johannes Freitag, Björn Sandrock
Format:	Article
Language:	English
Published:	MDPI AG 2021-02-01
Series:	Journal of Fungi
Subjects:	<i>Ustilago maydis</i> CRISPR/Cas9 genetic manipulation deletion point mutation
Online Access:	https://www.mdpi.com/2309-608X/7/2/149

Similar Items

Import and Export of Mannosylerythritol Lipids by Ustilago maydis
by: Fabienne Becker, et al.
Published: (2022-10-01)

Engineering Ustilago maydis for production of tailor-made mannosylerythritol lipids
by: Fabienne Becker, et al.
Published: (2021-06-01)

Understanding <i>Ustilago maydis</i> Infection of Multiple Maize Organs
by: Alex C Ferris, et al.
Published: (2020-12-01)

Chitosan Is Necessary for the Structure of the Cell Wall, and Full Virulence of <i>Ustilago maydis</i>
by: José Alejandro Sánchez-Arreguin, et al.
Published: (2022-08-01)

Carbon and Nitrogen Sources Have No Impact on the Organization and Composition of <i>Ustilago maydis</i> Respiratory Supercomplexes
by: Deyamira Matuz-Mares, et al.
Published: (2021-01-01)

Metabolic Changes and Antioxidant Response in <i>Ustilago maydis</i> Grown in Acetate
by: Lucero Romero-Aguilar, et al.
Published: (2023-07-01)

The Mitochondrial Alternative Oxidase in <i>Ustilago maydis</i> Is Not Involved in Response to Oxidative Stress Induced by Paraquat
by: Lucero Romero-Aguilar, et al.
Published: (2022-11-01)

Novel Secreted Effectors Conserved Among Smut Fungi Contribute to the Virulence of Ustilago maydis
by: Mariana Schuster, et al.
Published: (2024-03-01)

A New Method to Obtain Infective <i>Ustilago maydis</i> Binucleate Conidia for Corn Smut Production
by: Isaac Tello-Salgado, et al.
Published: (2024-04-01)

Structural and Functional Analysis of the Lectin-like Protein Llp1 Secreted by <i>Ustilago maydis</i> upon Infection of Maize
by: Marvin Christ, et al.
Published: (2025-02-01)

Effect of <i>Ustilago maydis</i> on the Nutritive Value and Aerobic Deterioration of Maize Silage
by: Lauksmė Merkevičiūte-Venslovė, et al.
Published: (2022-12-01)

mRNA Inventory of Extracellular Vesicles from <i>Ustilago maydis</i>
by: Seomun Kwon, et al.
Published: (2021-07-01)

Two Pex5 Proteins With Different Cargo Specificity Are Critical for Peroxisome Function in Ustilago maydis
by: Julia Ast, et al.
Published: (2022-05-01)

Root penetration of maize by Ustilago maydis
by: S.K. Sabbagh, et al.
Published: (2006-12-01)

<i>Ustilago maydis</i> Yeast Mutant Produces Cytosolic Melanin by Tyrosine-Tyrosinase Activity with Stain Biosorption Capability
by: Cinthya Martínez-López, et al.
Published: (2023-10-01)

A secreted Ustilago maydis effector promotes virulence by targeting anthocyanin biosynthesis in maize
by: Shigeyuki Tanaka, et al.
Published: (2014-01-01)

Acid pH Strategy Adaptation through <i>NRG1</i> in <i>Ustilago maydis</i>
by: José Alejandro Sánchez-Arreguin, et al.
Published: (2021-01-01)

Biomass pretreatment affects <it>Ustilago maydis </it>in producing itaconic acid
by: Klement Tobias, et al.
Published: (2012-04-01)

Integrated Transcriptome and GWAS Analysis to Identify Candidate Genes for <i>Ustilago maydis</i> Resistance in Maize
by: Bingyu Yin, et al.
Published: (2024-06-01)

The Fungal Protein Mes1 Is Required for Morphogenesis and Virulence in the Dimorphic Phytopathogen <i>Ustilago maydis</i>
by: David Cánovas
Published: (2022-07-01)

Identificación de las proteínas secretadas por el hongo Ustilago maydis (DeCandole) Corda (Basidiomiceto) cultivado en condiciones in vitro
by: Andrés Adolfo Estrada-Luna, et al.
Published: (2014-11-01)

Production of itaconic acid by Ustilago maydis from agro wastes in solid state fermentation
by: MOKULA MD. RAFI, et al.
Published: (2014-08-01)

Monitoring corn stover processing by the fungus Ustilago maydis
by: Stefan Robertz, et al.
Published: (2024-09-01)

A Novel Core Effector Vp1 Promotes Fungal Colonization and Virulence of <i>Ustilago maydis</i>
by: Cuong V. Hoang, et al.
Published: (2021-07-01)

<i>Ustilago maydis</i> Metabolic Characterization and Growth Quantification with a Genome-Scale Metabolic Model
by: Ulf W. Liebal, et al.
Published: (2022-05-01)

Fungal Pathogen Emergence: Investigations with an <i>Ustilago</i> <i>maydis</i> × <i>Sporisorium reilianum</i> Hybrid
by: Emilee R. M. Storfie, et al.
Published: (2021-08-01)

Early infection response of fungal biotroph Ustilago maydis in maize
by: Kunkun Zou, et al.
Published: (2022-09-01)

Klebsiella michiganensis: a nitrogen-fixing endohyphal bacterium from Ustilago maydis
by: Pengyu Liang, et al.
Published: (2023-12-01)

Response of Ustilago maydis against the Stress Caused by Three Polycationic Chitin Derivatives
by: Dario Rafael Olicón-Hernández, et al.
Published: (2017-12-01)

The <i>Ustilago hordei</i>–Barley Interaction is a Versatile System for Characterization of Fungal Effectors
by: Bilal Ökmen, et al.
Published: (2021-01-01)

Identification and Characterization of Two Transmembrane Proteins Required for Virulence of Ustilago maydis
by: Paul Weiland, et al.
Published: (2021-05-01)

Efecto de Trichoderma harzianum Rifai en el control de Ustilago maydis (DC.) Cda en maíz (Zea mays L.)
by: Ernesto Junior Pérez Torres, et al.
Published: (2014-05-01)

Functional Analysis of the Plasma Membrane H<sup>+</sup>-ATPases of <i>Ustilago maydis</i>
by: Melissa Vázquez-Carrada, et al.
Published: (2022-05-01)

Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
by: Kira Müntjes, et al.
Published: (2020-06-01)

Hungry for Sex: Differential Roles for <i>Ustilago maydis</i><i>b</i> Locus Components in Haploid Cells vis à vis Nutritional Availability
by: R. Margaret Wallen, et al.
Published: (2021-02-01)

Adaptation of Ustilago maydis to phenolic and alkaloid responsive metabolites in maize B73
by: Xuanyue Guo, et al.
Published: (2024-07-01)

Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis
by: Kishor D. Ingole, et al.
Published: (2022-10-01)

The production and secretion of tRNA-derived RNA fragments in the corn smut fungus Ustilago maydis
by: Rei Yoshimoto, et al.
Published: (2022-08-01)

A Novel Potent Carrier for Unconventional Protein Export in Ustilago maydis
by: Magnus Philipp, et al.
Published: (2022-01-01)

Single telomere length analysis in Ustilago maydis, a high-resolution tool for examining fungal telomere length distribution and C-strand 5’-end processing
by: Ganduri Swapna, et al.
Published: (2018-08-01)