<i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites
Marine bacteria are a significant source of bioactive compounds for various biotechnological applications. Among these, actinomycetes have been found to produce a wide range of secondary metabolites of interest. <i>Saccharopolyspora</i> is one of the genera of actinomycetes that has been...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/902 |
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| author | Constança D. F. Bertrand Rodrigo Martins Francisco Quintas-Nunes Pedro Reynolds-Brandão Maria T. B. Crespo Francisco X. Nascimento |
| author_facet | Constança D. F. Bertrand Rodrigo Martins Francisco Quintas-Nunes Pedro Reynolds-Brandão Maria T. B. Crespo Francisco X. Nascimento |
| author_sort | Constança D. F. Bertrand |
| collection | DOAJ |
| description | Marine bacteria are a significant source of bioactive compounds for various biotechnological applications. Among these, actinomycetes have been found to produce a wide range of secondary metabolites of interest. <i>Saccharopolyspora</i> is one of the genera of actinomycetes that has been recognized as a potential source of these compounds. This study reports the characterization and genomic analysis of <i>Saccharopolyspora</i> sp. NFXS83, a marine bacterium isolated from seawater from the Sado estuary in Portugal. The NFXS83 strain produced multiple functional and stable extracellular enzymes under high-salt conditions, showed the ability to synthesize auxins such as indole-3-acetic acid, and produced diffusible secondary metabolites capable of inhibiting the growth of <i>Staphylococcus aureus</i>. Furthermore, when <i>Phaeodactylum tricornutum</i> was co-cultivated with strain NFXS83 a significant increase in microalgae cell count, cell size, auto-fluorescence, and fucoxanthin content was observed. Detailed analysis revealed the presence of clusters involved in the production of various secondary metabolites, including extracellular enzymes, antimicrobial compounds, terpenes, and carotenoids in the genome of strain NFXS83. Ultimately, these findings indicate that <i>Saccharopolyspora</i> sp. NFXS83 has a significant potential for a wide range of marine biotechnological applications. |
| first_indexed | 2024-03-11T04:43:48Z |
| format | Article |
| id | doaj.art-aa5d7b5acaf6420893bf29b2f528c1cf |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:48Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-aa5d7b5acaf6420893bf29b2f528c1cf2023-11-17T20:32:10ZengMDPI AGMicroorganisms2076-26072023-03-0111490210.3390/microorganisms11040902<i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary MetabolitesConstança D. F. Bertrand0Rodrigo Martins1Francisco Quintas-Nunes2Pedro Reynolds-Brandão3Maria T. B. Crespo4Francisco X. Nascimento5iBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugaliBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugaliBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugaliBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugaliBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugaliBET—Instituto de Biologia Experimental e Tecnológica, 2781-901 Oeiras, PortugalMarine bacteria are a significant source of bioactive compounds for various biotechnological applications. Among these, actinomycetes have been found to produce a wide range of secondary metabolites of interest. <i>Saccharopolyspora</i> is one of the genera of actinomycetes that has been recognized as a potential source of these compounds. This study reports the characterization and genomic analysis of <i>Saccharopolyspora</i> sp. NFXS83, a marine bacterium isolated from seawater from the Sado estuary in Portugal. The NFXS83 strain produced multiple functional and stable extracellular enzymes under high-salt conditions, showed the ability to synthesize auxins such as indole-3-acetic acid, and produced diffusible secondary metabolites capable of inhibiting the growth of <i>Staphylococcus aureus</i>. Furthermore, when <i>Phaeodactylum tricornutum</i> was co-cultivated with strain NFXS83 a significant increase in microalgae cell count, cell size, auto-fluorescence, and fucoxanthin content was observed. Detailed analysis revealed the presence of clusters involved in the production of various secondary metabolites, including extracellular enzymes, antimicrobial compounds, terpenes, and carotenoids in the genome of strain NFXS83. Ultimately, these findings indicate that <i>Saccharopolyspora</i> sp. NFXS83 has a significant potential for a wide range of marine biotechnological applications.https://www.mdpi.com/2076-2607/11/4/902<i>Saccharopolyspora</i>marinemicroalgaesecondary metabolitesbiotechnology |
| spellingShingle | Constança D. F. Bertrand Rodrigo Martins Francisco Quintas-Nunes Pedro Reynolds-Brandão Maria T. B. Crespo Francisco X. Nascimento <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites Microorganisms <i>Saccharopolyspora</i> marine microalgae secondary metabolites biotechnology |
| title | <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites |
| title_full | <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites |
| title_fullStr | <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites |
| title_full_unstemmed | <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites |
| title_short | <i>Saccharopolyspora</i> sp. NFXS83 in Marine Biotechnological Applications: From Microalgae Growth Promotion to the Production of Secondary Metabolites |
| title_sort | i saccharopolyspora i sp nfxs83 in marine biotechnological applications from microalgae growth promotion to the production of secondary metabolites |
| topic | <i>Saccharopolyspora</i> marine microalgae secondary metabolites biotechnology |
| url | https://www.mdpi.com/2076-2607/11/4/902 |
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