Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures
The function of proanthocyanidins (PAs) relies on their structure and requires high-purity PAs. Though Sephadex LH-20 gel permeation chromatography (GPC) is expected to separate PAs based on structure, its usage rules and mechanisms remain unclear. This study delves into the PAs separation patterns...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523004510 |
| _version_ | 1797383673741312000 |
|---|---|
| author | Xiaoyi Chen Hong Song Shubo Zhou Chunlong Yuan Junjun Li |
| author_facet | Xiaoyi Chen Hong Song Shubo Zhou Chunlong Yuan Junjun Li |
| author_sort | Xiaoyi Chen |
| collection | DOAJ |
| description | The function of proanthocyanidins (PAs) relies on their structure and requires high-purity PAs. Though Sephadex LH-20 gel permeation chromatography (GPC) is expected to separate PAs based on structure, its usage rules and mechanisms remain unclear. This study delves into the PAs separation patterns on Sephadex LH-20, first confirming the purification mechanisms of PAs with various mean degrees of polymerization (DP) using the adsorption kinetic model. The study found that an increase in the molecular weight or mean DP of PAs results in decreased polarity, reduced hydrogen bonding actions, and intensified hydrophobic effect, causing delayed extraction of PAs on Sephadex LH-20, with galloylated PA as an exception, which was extracted first despite its high DP. Additionally, the principles for separating specific composition, such as monomers, dimers, etc., were evaluated. The study sheds light on enhancing the purification efficiency of PAs, thus advancing the precise separation technology of diverse proanthocyanidins. |
| first_indexed | 2024-03-08T21:25:23Z |
| format | Article |
| id | doaj.art-aa5ee4b358fe4836b9355f67e846464f |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-03-08T21:25:23Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-aa5ee4b358fe4836b9355f67e846464f2023-12-21T07:36:56ZengElsevierFood Chemistry: X2590-15752023-12-0120101008Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structuresXiaoyi Chen0Hong Song1Shubo Zhou2Chunlong Yuan3Junjun Li4College of Enology, Northwest A&F University, Yangling 712100, ChinaCollege of Enology, Northwest A&F University, Yangling 712100, ChinaCollege of Enology, Northwest A&F University, Yangling 712100, ChinaCollege of Enology, Northwest A&F University, Yangling 712100, China; Ningxia Helan Mountain's East Foothill Wine Experiment and Demonstration Station of Northwest A&F University, Yongning, Ningxia 750104, China; Corresponding authors at: College of Enology, Northwest A&F University, No. 22, Xinong Road, Yangling, Shaanxi 710000, China.College of Enology, Northwest A&F University, Yangling 712100, China; Corresponding authors at: College of Enology, Northwest A&F University, No. 22, Xinong Road, Yangling, Shaanxi 710000, China.The function of proanthocyanidins (PAs) relies on their structure and requires high-purity PAs. Though Sephadex LH-20 gel permeation chromatography (GPC) is expected to separate PAs based on structure, its usage rules and mechanisms remain unclear. This study delves into the PAs separation patterns on Sephadex LH-20, first confirming the purification mechanisms of PAs with various mean degrees of polymerization (DP) using the adsorption kinetic model. The study found that an increase in the molecular weight or mean DP of PAs results in decreased polarity, reduced hydrogen bonding actions, and intensified hydrophobic effect, causing delayed extraction of PAs on Sephadex LH-20, with galloylated PA as an exception, which was extracted first despite its high DP. Additionally, the principles for separating specific composition, such as monomers, dimers, etc., were evaluated. The study sheds light on enhancing the purification efficiency of PAs, thus advancing the precise separation technology of diverse proanthocyanidins.http://www.sciencedirect.com/science/article/pii/S2590157523004510ProanthocyanidinsAdsorption kineticsReverse chromatographySephadex LH-20 |
| spellingShingle | Xiaoyi Chen Hong Song Shubo Zhou Chunlong Yuan Junjun Li Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures Food Chemistry: X Proanthocyanidins Adsorption kinetics Reverse chromatography Sephadex LH-20 |
| title | Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures |
| title_full | Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures |
| title_fullStr | Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures |
| title_full_unstemmed | Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures |
| title_short | Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures |
| title_sort | exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights compositions and structures |
| topic | Proanthocyanidins Adsorption kinetics Reverse chromatography Sephadex LH-20 |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523004510 |
| work_keys_str_mv | AT xiaoyichen exploringseparationpatternsandmechanismsofproanthocyanidinsingrapeseedsandpomacewithdiversemolecularweightscompositionsandstructures AT hongsong exploringseparationpatternsandmechanismsofproanthocyanidinsingrapeseedsandpomacewithdiversemolecularweightscompositionsandstructures AT shubozhou exploringseparationpatternsandmechanismsofproanthocyanidinsingrapeseedsandpomacewithdiversemolecularweightscompositionsandstructures AT chunlongyuan exploringseparationpatternsandmechanismsofproanthocyanidinsingrapeseedsandpomacewithdiversemolecularweightscompositionsandstructures AT junjunli exploringseparationpatternsandmechanismsofproanthocyanidinsingrapeseedsandpomacewithdiversemolecularweightscompositionsandstructures |