Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts
The inflammatory process, although beneficial, can produce tissue damage and systemic damage when uncontrolled. Effective therapeutic alternatives with little or no side effects are of great therapeutic interest. This study aimed to determine the phytochemical composition of bark extracts from <i...
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MDPI AG
2023-01-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/3/560 |
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| author | Yazmín B. Jiménez-Nevárez Miguel Angel Angulo-Escalante Julio Montes-Avila Araceli Guerrero-Alonso Judith González Christen Israel Hurtado-Díaz J. Basilio Heredia Eber Addí Quintana-Obregón Laura Alvarez |
| author_facet | Yazmín B. Jiménez-Nevárez Miguel Angel Angulo-Escalante Julio Montes-Avila Araceli Guerrero-Alonso Judith González Christen Israel Hurtado-Díaz J. Basilio Heredia Eber Addí Quintana-Obregón Laura Alvarez |
| author_sort | Yazmín B. Jiménez-Nevárez |
| collection | DOAJ |
| description | The inflammatory process, although beneficial, can produce tissue damage and systemic damage when uncontrolled. Effective therapeutic alternatives with little or no side effects are of great therapeutic interest. This study aimed to determine the phytochemical composition of bark extracts from <i>J. cordata</i>, an endemic plant from México, and evaluate their in vitro anti-inflammatory activity. Hexane, ethyl acetate, and methanol extracts were characterized by qualitative phytochemical tests, and their bioactive groups were identified by <sup>1</sup>H NMR and gas chromatography coupled to mass spectrometry (GC–MS). The extract’s anti-inflammatory activity was evaluated as nitric oxide (NO) production and their cytotoxicity by an MTS cell proliferation assay in lipopolysaccharide (LPS)-activated RAW 264.7 cells at concentrations of 1–100 μg/mL. The hexane extract contained fatty acids, fatty esters, phytosterols, alkanes, vitamin E, and terpenoids; the ethyl acetate extract showed fatty acids, fatty esters, aromatic aldehyde, phytosterols, vitamin E, and terpenoids, while the methanolic extract showed fatty esters, fatty acid, aromatics aldehydes, and alcohol. The ethyl acetate extract showed the highest inhibition of NO production, followed by the methanolic extract and the hexane extract, without affecting the viability of RAW 264.7 macrophage cells. The results suggest that <i>J. cordata</i> extracts are a potential source of bioactive compounds with anti-inflammatory potential. |
| first_indexed | 2024-03-11T09:29:39Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:39Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-aa64b06d57644cf599cae706299896a42023-11-16T17:44:07ZengMDPI AGPlants2223-77472023-01-0112356010.3390/plants12030560Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark ExtractsYazmín B. Jiménez-Nevárez0Miguel Angel Angulo-Escalante1Julio Montes-Avila2Araceli Guerrero-Alonso3Judith González Christen4Israel Hurtado-Díaz5J. Basilio Heredia6Eber Addí Quintana-Obregón7Laura Alvarez8Centro de Investigación en Alimentación y Desarrollo A.C. Carretera Eldorado km 5.5, Campo El Diez, Culiacán 80110, MexicoCentro de Investigación en Alimentación y Desarrollo A.C. Carretera Eldorado km 5.5, Campo El Diez, Culiacán 80110, MexicoPrograma de Posgrado en Ciencias Biomédicas, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Sinaloa, Ciudad Universitaria s/n, Culiacán 80010, MexicoCentro de Investigaciones Químicas IICBA, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, Cuernavaca 62209, MexicoLaboratorio de Inmunidad Innata, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, C.P., Cuernavaca 62209, MexicoDepartamento de Madera Celulosa y Papel, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Km 15.5 Guadalajara-Nogales, Las Agujas, Zapopan 45100, MexicoCentro de Investigación en Alimentación y Desarrollo A.C. Carretera Eldorado km 5.5, Campo El Diez, Culiacán 80110, MexicoCONACYT-Centro de Investigación en Alimentación y Desarrollo A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Col. La Victoria, Hermosillo 83304, MexicoCentro de Investigaciones Químicas IICBA, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Col. Chamilpa, Cuernavaca 62209, MexicoThe inflammatory process, although beneficial, can produce tissue damage and systemic damage when uncontrolled. Effective therapeutic alternatives with little or no side effects are of great therapeutic interest. This study aimed to determine the phytochemical composition of bark extracts from <i>J. cordata</i>, an endemic plant from México, and evaluate their in vitro anti-inflammatory activity. Hexane, ethyl acetate, and methanol extracts were characterized by qualitative phytochemical tests, and their bioactive groups were identified by <sup>1</sup>H NMR and gas chromatography coupled to mass spectrometry (GC–MS). The extract’s anti-inflammatory activity was evaluated as nitric oxide (NO) production and their cytotoxicity by an MTS cell proliferation assay in lipopolysaccharide (LPS)-activated RAW 264.7 cells at concentrations of 1–100 μg/mL. The hexane extract contained fatty acids, fatty esters, phytosterols, alkanes, vitamin E, and terpenoids; the ethyl acetate extract showed fatty acids, fatty esters, aromatic aldehyde, phytosterols, vitamin E, and terpenoids, while the methanolic extract showed fatty esters, fatty acid, aromatics aldehydes, and alcohol. The ethyl acetate extract showed the highest inhibition of NO production, followed by the methanolic extract and the hexane extract, without affecting the viability of RAW 264.7 macrophage cells. The results suggest that <i>J. cordata</i> extracts are a potential source of bioactive compounds with anti-inflammatory potential.https://www.mdpi.com/2223-7747/12/3/560<i>Jatropha cordata</i>anti-inflammatory activityNO production inhibitionterpenesgas chromatography–mass spectrometryNMR |
| spellingShingle | Yazmín B. Jiménez-Nevárez Miguel Angel Angulo-Escalante Julio Montes-Avila Araceli Guerrero-Alonso Judith González Christen Israel Hurtado-Díaz J. Basilio Heredia Eber Addí Quintana-Obregón Laura Alvarez Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts Plants <i>Jatropha cordata</i> anti-inflammatory activity NO production inhibition terpenes gas chromatography–mass spectrometry NMR |
| title | Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts |
| title_full | Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts |
| title_fullStr | Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts |
| title_full_unstemmed | Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts |
| title_short | Phytochemical Characterization and In Vitro Anti-Inflammatory Evaluation in RAW 264.7 Cells of <i>Jatropha cordata</i> Bark Extracts |
| title_sort | phytochemical characterization and in vitro anti inflammatory evaluation in raw 264 7 cells of i jatropha cordata i bark extracts |
| topic | <i>Jatropha cordata</i> anti-inflammatory activity NO production inhibition terpenes gas chromatography–mass spectrometry NMR |
| url | https://www.mdpi.com/2223-7747/12/3/560 |
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