AFM and MFM techniques for enzyme activity imaging and quantification
In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The r...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-07-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2018.1470904 |
| _version_ | 1818047254470066176 |
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| author | Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina |
| author_facet | Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina |
| author_sort | Michelle Arredondo |
| collection | DOAJ |
| description | In this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained. |
| first_indexed | 2024-12-10T10:02:52Z |
| format | Article |
| id | doaj.art-aa6563ce20cf4c2dae050d903fad4ba6 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-10T10:02:52Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-aa6563ce20cf4c2dae050d903fad4ba62022-12-22T01:53:20ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302018-07-013241065107410.1080/13102818.2018.14709041470904AFM and MFM techniques for enzyme activity imaging and quantificationMichelle Arredondo0Margarita Stoytcheva1Israel Morales-Reyes2Nikola Batina3Tecnológico Nacional de MéxicoUniversidad Autónoma de Baja CaliforniaDivisión de Ciencias Básicas e IngenieríaDivisión de Ciencias Básicas e IngenieríaIn this work, a new approach for enzyme activity monitoring is suggested. It is based on the real-time imaging, by atomic force microscopy (AFM) and magnetic force microscopy (MFM), of the degradation of a nanoparticles-loaded enzyme responsive layer, namely Fe3O4-nanoparticles-loaded gelatin. The roughness analysis allowed the quantification of the trypsin-induced ferrogel degradation and the correlation of the AFM and MFM data obtained.http://dx.doi.org/10.1080/13102818.2018.1470904Atomic force microscopymagnetic force microscopytrypsin activityferrogel |
| spellingShingle | Michelle Arredondo Margarita Stoytcheva Israel Morales-Reyes Nikola Batina AFM and MFM techniques for enzyme activity imaging and quantification Biotechnology & Biotechnological Equipment Atomic force microscopy magnetic force microscopy trypsin activity ferrogel |
| title | AFM and MFM techniques for enzyme activity imaging and quantification |
| title_full | AFM and MFM techniques for enzyme activity imaging and quantification |
| title_fullStr | AFM and MFM techniques for enzyme activity imaging and quantification |
| title_full_unstemmed | AFM and MFM techniques for enzyme activity imaging and quantification |
| title_short | AFM and MFM techniques for enzyme activity imaging and quantification |
| title_sort | afm and mfm techniques for enzyme activity imaging and quantification |
| topic | Atomic force microscopy magnetic force microscopy trypsin activity ferrogel |
| url | http://dx.doi.org/10.1080/13102818.2018.1470904 |
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