A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation
Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method whi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2018-01-01
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| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172455 |
| _version_ | 1818341140613562368 |
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| author | Wen Hou Lei Liu Xiaohong Zhang Chuanfa Liu |
| author_facet | Wen Hou Lei Liu Xiaohong Zhang Chuanfa Liu |
| author_sort | Wen Hou |
| collection | DOAJ |
| description | Sequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams. |
| first_indexed | 2024-12-13T15:54:04Z |
| format | Article |
| id | doaj.art-aa65e493df7a467cb62886ac8bdb7fdd |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-13T15:54:04Z |
| publishDate | 2018-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-aa65e493df7a467cb62886ac8bdb7fdd2022-12-21T23:39:22ZengThe Royal SocietyRoyal Society Open Science2054-57032018-01-015610.1098/rsos.172455172455A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligationWen HouLei LiuXiaohong ZhangChuanfa LiuSequential peptide ligation strategy becomes more and more important in large protein or long peptides chemical synthesis due to the limited peptide/protein size obtained by solid phase synthesis of individual peptides or even one-step peptide ligation. Herein, we developed an alternative method which could perform the sequential peptide ligation of several segments from N to C direction based on the combined use of thioacid capture ligation and native chemical ligation. The sweet protein monellin was produced through this strategy on a scale of multi-milligrams.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172455sequential peptide ligationthioacid capture ligationnative chemical ligationprotein chemical synthesis |
| spellingShingle | Wen Hou Lei Liu Xiaohong Zhang Chuanfa Liu A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation Royal Society Open Science sequential peptide ligation thioacid capture ligation native chemical ligation protein chemical synthesis |
| title | A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation |
| title_full | A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation |
| title_fullStr | A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation |
| title_full_unstemmed | A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation |
| title_short | A new method of N to C sequential ligation using thioacid capture ligation and native chemical ligation |
| title_sort | new method of n to c sequential ligation using thioacid capture ligation and native chemical ligation |
| topic | sequential peptide ligation thioacid capture ligation native chemical ligation protein chemical synthesis |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.172455 |
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