Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils
Periodontitis is a highly prevalent infectious disease that affects ~ 50% of the adults in the USA alone. Two Gram-positive anaerobic oral bacteria, <i>Filifactor alocis</i> and <i>Peptoanaerobacter</i> <i>stomatis</i>, have emerged as important periodontal pathog...
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| Format: | Article |
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MDPI AG
2019-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/2/59 |
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| author | Aruna Vashishta Emeri Jimenez-Flores Christopher K. Klaes Shifu Tian Irina Miralda Richard J. Lamont Silvia M. Uriarte |
| author_facet | Aruna Vashishta Emeri Jimenez-Flores Christopher K. Klaes Shifu Tian Irina Miralda Richard J. Lamont Silvia M. Uriarte |
| author_sort | Aruna Vashishta |
| collection | DOAJ |
| description | Periodontitis is a highly prevalent infectious disease that affects ~ 50% of the adults in the USA alone. Two Gram-positive anaerobic oral bacteria, <i>Filifactor alocis</i> and <i>Peptoanaerobacter</i> <i>stomatis</i>, have emerged as important periodontal pathogens. Neutrophils are a major component of the innate host response in the gingival tissue, and the contribution of neutrophil-derived cytokines and chemokines plays a central role in disease progression. The pattern of cytokines and chemokines released by human neutrophils upon stimulation with newly appreciated periodontal bacteria compared to the keystone oral pathogen <i>Porphyromonas gingivalis</i> was investigated. Our results showed that both <i>F. alocis</i> and <i>P. stomatis</i> triggered TLR2/6 activation. <i>F. alocis</i> induced significant changes in gene expression of cytokines and chemokines in human neutrophils compared to unstimulated cells. However, except for IL-1ra, neutrophils released lower levels of cytokines and chemokines in response to <i>F. alocis</i> compared to <i>P. stomatis.</i> Furthermore, bacteria-free conditioned supernatant collected from neutrophils challenged with <i>P. stomatis</i>, but not from <i>P. gingivalis</i> or <i>F. alocis,</i> was chemotactic towards both neutrophils and monocytes. Elucidating stimuli-specific modulation of human neutrophil effector functions in the context of dysbiotic microbial community constituents provides valuable information for understanding the pathogenesis of periodontal diseases. |
| first_indexed | 2024-04-14T05:16:42Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-14T05:16:42Z |
| publishDate | 2019-05-01 |
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| series | Pathogens |
| spelling | doaj.art-aa67ce5fb326450dadfeefde458f9fc52022-12-22T02:10:20ZengMDPI AGPathogens2076-08172019-05-01825910.3390/pathogens8020059pathogens8020059Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human NeutrophilsAruna Vashishta0Emeri Jimenez-Flores1Christopher K. Klaes2Shifu Tian3Irina Miralda4Richard J. Lamont5Silvia M. Uriarte6Department of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USADepartment of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USADepartment of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USADepartment of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USADepartment of Microbiology & Immunology, School of Medicine, University of Louisville, 505 S. Hancock St., Louisville, KY 40202, USADepartment of Oral Immunology and Infectious Diseases, School of Dentistry, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USADepartment of Medicine, School of Medicine, University of Louisville, 570 S. Preston St., Louisville, KY 40202, USAPeriodontitis is a highly prevalent infectious disease that affects ~ 50% of the adults in the USA alone. Two Gram-positive anaerobic oral bacteria, <i>Filifactor alocis</i> and <i>Peptoanaerobacter</i> <i>stomatis</i>, have emerged as important periodontal pathogens. Neutrophils are a major component of the innate host response in the gingival tissue, and the contribution of neutrophil-derived cytokines and chemokines plays a central role in disease progression. The pattern of cytokines and chemokines released by human neutrophils upon stimulation with newly appreciated periodontal bacteria compared to the keystone oral pathogen <i>Porphyromonas gingivalis</i> was investigated. Our results showed that both <i>F. alocis</i> and <i>P. stomatis</i> triggered TLR2/6 activation. <i>F. alocis</i> induced significant changes in gene expression of cytokines and chemokines in human neutrophils compared to unstimulated cells. However, except for IL-1ra, neutrophils released lower levels of cytokines and chemokines in response to <i>F. alocis</i> compared to <i>P. stomatis.</i> Furthermore, bacteria-free conditioned supernatant collected from neutrophils challenged with <i>P. stomatis</i>, but not from <i>P. gingivalis</i> or <i>F. alocis,</i> was chemotactic towards both neutrophils and monocytes. Elucidating stimuli-specific modulation of human neutrophil effector functions in the context of dysbiotic microbial community constituents provides valuable information for understanding the pathogenesis of periodontal diseases.https://www.mdpi.com/2076-0817/8/2/59Emerging oral pathogenshuman neutrophilscytokines and chemokineschemotaxis |
| spellingShingle | Aruna Vashishta Emeri Jimenez-Flores Christopher K. Klaes Shifu Tian Irina Miralda Richard J. Lamont Silvia M. Uriarte Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils Pathogens Emerging oral pathogens human neutrophils cytokines and chemokines chemotaxis |
| title | Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils |
| title_full | Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils |
| title_fullStr | Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils |
| title_full_unstemmed | Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils |
| title_short | Putative Periodontal Pathogens, <i>Filifactor alocis</i> and <i>Peptoanaerobacter stomatis,</i> Induce Differential Cytokine and Chemokine Production by Human Neutrophils |
| title_sort | putative periodontal pathogens i filifactor alocis i and i peptoanaerobacter stomatis i induce differential cytokine and chemokine production by human neutrophils |
| topic | Emerging oral pathogens human neutrophils cytokines and chemokines chemotaxis |
| url | https://www.mdpi.com/2076-0817/8/2/59 |
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