Urokinase-type plasminogen activator: a new target for male contraception?
Urokinase-type plasminogen activator (uPA) is closely related to male reproduction. With the aim of investigating the possibility for uPA as a potential contraceptive target, in the present work, Kunming male mice were immunized by human uPA subcutaneous injection at three separate doses for 3 times...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-04-01
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| Series: | Asian Journal of Andrology |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=2;spage=269;epage=273;aulast=Qin |
| _version_ | 1811327023105179648 |
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| author | Ying Qin Yan Han Cheng-Liang Xiong Hong-Gang Li Lian Hu Ling Zhang |
| author_facet | Ying Qin Yan Han Cheng-Liang Xiong Hong-Gang Li Lian Hu Ling Zhang |
| author_sort | Ying Qin |
| collection | DOAJ |
| description | Urokinase-type plasminogen activator (uPA) is closely related to male reproduction. With the aim of investigating the possibility for uPA as a potential contraceptive target, in the present work, Kunming male mice were immunized by human uPA subcutaneous injection at three separate doses for 3 times. Then the potency of the anti-human uPA antibody in serum was analyzed, and mouse fertility was evaluated. Serum antibody titers for human uPA in immunized groups all reached 1:10,240 or higher levels by enzyme linked immunosorbent assay, and mating experiments revealed that pregnancy rates and the mean number of embryos implanted after mating declined obviously (P < 0.05) when compared with control groups. However, the mating capacity and reproductive organ weights had no obvious change, and histological analysis of the testes and epididymides also showed normal morphology for immunized male mice. Sperm function tests suggested that the sperm concentration, sperm viability, sperm motility, and in vitro fertilization rate for the cauda epididymis sperm in uPA-immunized groups were lower than those in the controls (P < 0.05). Together, these observations indicated that subcutaneous injection human uPA to the male mice could effectively reduce their fertility, and uPA could become a new target for immunocontraception in male contraceptive development. |
| first_indexed | 2024-04-13T14:59:16Z |
| format | Article |
| id | doaj.art-aa6e58a8f6f24205b3bc8f6dea0db70f |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-04-13T14:59:16Z |
| publishDate | 2015-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-aa6e58a8f6f24205b3bc8f6dea0db70f2022-12-22T02:42:21ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622015-04-0117226927310.4103/1008-682X.143316Urokinase-type plasminogen activator: a new target for male contraception?Ying Qin Yan Han Cheng-Liang Xiong Hong-Gang Li Lian HuLing ZhangUrokinase-type plasminogen activator (uPA) is closely related to male reproduction. With the aim of investigating the possibility for uPA as a potential contraceptive target, in the present work, Kunming male mice were immunized by human uPA subcutaneous injection at three separate doses for 3 times. Then the potency of the anti-human uPA antibody in serum was analyzed, and mouse fertility was evaluated. Serum antibody titers for human uPA in immunized groups all reached 1:10,240 or higher levels by enzyme linked immunosorbent assay, and mating experiments revealed that pregnancy rates and the mean number of embryos implanted after mating declined obviously (P < 0.05) when compared with control groups. However, the mating capacity and reproductive organ weights had no obvious change, and histological analysis of the testes and epididymides also showed normal morphology for immunized male mice. Sperm function tests suggested that the sperm concentration, sperm viability, sperm motility, and in vitro fertilization rate for the cauda epididymis sperm in uPA-immunized groups were lower than those in the controls (P < 0.05). Together, these observations indicated that subcutaneous injection human uPA to the male mice could effectively reduce their fertility, and uPA could become a new target for immunocontraception in male contraceptive development.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=2;spage=269;epage=273;aulast=Qin |
| spellingShingle | Ying Qin Yan Han Cheng-Liang Xiong Hong-Gang Li Lian Hu Ling Zhang Urokinase-type plasminogen activator: a new target for male contraception? Asian Journal of Andrology |
| title | Urokinase-type plasminogen activator: a new target for male contraception? |
| title_full | Urokinase-type plasminogen activator: a new target for male contraception? |
| title_fullStr | Urokinase-type plasminogen activator: a new target for male contraception? |
| title_full_unstemmed | Urokinase-type plasminogen activator: a new target for male contraception? |
| title_short | Urokinase-type plasminogen activator: a new target for male contraception? |
| title_sort | urokinase type plasminogen activator a new target for male contraception |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2015;volume=17;issue=2;spage=269;epage=273;aulast=Qin |
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