Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes.
Retrotransposition of the budding yeast long terminal repeat retrotransposon Ty3 is activated during mating. In this study, proteins that associate with Ty3 Gag3 capsid protein during virus-like particle (VLP) assembly were identified by mass spectrometry and screened for roles in mating-stimulated...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4589538?pdf=render |
| _version_ | 1818516719685074944 |
|---|---|
| author | Virginia Bilanchone Kristina Clemens Robyn Kaake Anthony R Dawson Dina Matheos Kunio Nagashima Parth Sitlani Kurt Patterson Ivan Chang Lan Huang Suzanne Sandmeyer |
| author_facet | Virginia Bilanchone Kristina Clemens Robyn Kaake Anthony R Dawson Dina Matheos Kunio Nagashima Parth Sitlani Kurt Patterson Ivan Chang Lan Huang Suzanne Sandmeyer |
| author_sort | Virginia Bilanchone |
| collection | DOAJ |
| description | Retrotransposition of the budding yeast long terminal repeat retrotransposon Ty3 is activated during mating. In this study, proteins that associate with Ty3 Gag3 capsid protein during virus-like particle (VLP) assembly were identified by mass spectrometry and screened for roles in mating-stimulated retrotransposition. Components of RNA processing bodies including DEAD box helicases Dhh1/DDX6 and Ded1/DDX3, Sm-like protein Lsm1, decapping protein Dcp2, and 5' to 3' exonuclease Xrn1 were among the proteins identified. These proteins associated with Ty3 proteins and RNA, and were required for formation of Ty3 VLP retrosome assembly factories and for retrotransposition. Specifically, Dhh1/DDX6 was required for normal levels of Ty3 genomic RNA, and Lsm1 and Xrn1 were required for association of Ty3 protein and RNA into retrosomes. This role for components of RNA processing bodies in promoting VLP assembly and retrotransposition during mating in a yeast that lacks RNA interference, contrasts with roles proposed for orthologous components in animal germ cell ribonucleoprotein granules in turnover and epigenetic suppression of retrotransposon RNAs. |
| first_indexed | 2024-12-11T00:45:58Z |
| format | Article |
| id | doaj.art-aa70de8f420a474d95b9d993c5f5be99 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T00:45:58Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-aa70de8f420a474d95b9d993c5f5be992022-12-22T01:26:46ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-01-01119e100552810.1371/journal.pgen.1005528Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes.Virginia BilanchoneKristina ClemensRobyn KaakeAnthony R DawsonDina MatheosKunio NagashimaParth SitlaniKurt PattersonIvan ChangLan HuangSuzanne SandmeyerRetrotransposition of the budding yeast long terminal repeat retrotransposon Ty3 is activated during mating. In this study, proteins that associate with Ty3 Gag3 capsid protein during virus-like particle (VLP) assembly were identified by mass spectrometry and screened for roles in mating-stimulated retrotransposition. Components of RNA processing bodies including DEAD box helicases Dhh1/DDX6 and Ded1/DDX3, Sm-like protein Lsm1, decapping protein Dcp2, and 5' to 3' exonuclease Xrn1 were among the proteins identified. These proteins associated with Ty3 proteins and RNA, and were required for formation of Ty3 VLP retrosome assembly factories and for retrotransposition. Specifically, Dhh1/DDX6 was required for normal levels of Ty3 genomic RNA, and Lsm1 and Xrn1 were required for association of Ty3 protein and RNA into retrosomes. This role for components of RNA processing bodies in promoting VLP assembly and retrotransposition during mating in a yeast that lacks RNA interference, contrasts with roles proposed for orthologous components in animal germ cell ribonucleoprotein granules in turnover and epigenetic suppression of retrotransposon RNAs.http://europepmc.org/articles/PMC4589538?pdf=render |
| spellingShingle | Virginia Bilanchone Kristina Clemens Robyn Kaake Anthony R Dawson Dina Matheos Kunio Nagashima Parth Sitlani Kurt Patterson Ivan Chang Lan Huang Suzanne Sandmeyer Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. PLoS Genetics |
| title | Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. |
| title_full | Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. |
| title_fullStr | Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. |
| title_full_unstemmed | Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. |
| title_short | Ty3 Retrotransposon Hijacks Mating Yeast RNA Processing Bodies to Infect New Genomes. |
| title_sort | ty3 retrotransposon hijacks mating yeast rna processing bodies to infect new genomes |
| url | http://europepmc.org/articles/PMC4589538?pdf=render |
| work_keys_str_mv | AT virginiabilanchone ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT kristinaclemens ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT robynkaake ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT anthonyrdawson ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT dinamatheos ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT kunionagashima ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT parthsitlani ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT kurtpatterson ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT ivanchang ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT lanhuang ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes AT suzannesandmeyer ty3retrotransposonhijacksmatingyeastrnaprocessingbodiestoinfectnewgenomes |