Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1
BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radi...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Secretariat of The Indonesian Biomedical Journal
2021-03-01
|
| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/1428 |
| _version_ | 1819175397769609216 |
|---|---|
| author | Dimpuulina Erna Mariati Sunarjati Sudigdoadi Ronny Lesmana Astrid Feinisa Khairani Julia Windi Gunadi Vita Murniati Tarawan Unang Supratman Hanna Goenawan |
| author_facet | Dimpuulina Erna Mariati Sunarjati Sudigdoadi Ronny Lesmana Astrid Feinisa Khairani Julia Windi Gunadi Vita Murniati Tarawan Unang Supratman Hanna Goenawan |
| author_sort | Dimpuulina Erna Mariati |
| collection | DOAJ |
| description | BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radiation is still limited. Therefore, the aim of this study was to examine the effect of robusta extract cream (RE cream) on preventing wrinkle in mice induced by ultraviolet-B (UVB) radiation.
METHODS: RE cream was made by mixing RE coffee with moisturizing cream in different concentration (10%, 20%, and 40%). Twenty-five male of Mus musculus Balb/c strain mice aged 4 weeks were divided into five groups; control group, UVB group, UVB + 10% RE group, UVB + 20% RE group, and UVB + 40% RE group. The UVB groups were given UVB radiation three times a week with an exposure duration of 100 seconds per time for ten weeks. At the end of the treatment, skin samples were excised and statined histologically, also were analyzed for their protein expression. Evaluation of wrinkles was carried out using the Bissete method before and after treatment. To evaluate the thickness of the epidermis, HE staining was performed, while masson Trichome staining was performed to determine the collagen content.
RESULTS: RE cream-treated groups showed lower wrinkle score compared to the control group. Furthermore, in UVB + 10% RE group, the RE cream application reduce wrinkle formation. In UVB + 10% RE group and UVB + 20% RE group, the RE cream application increased epidermal thickness and collagen content (p=0.00). While collagenase, matrix metalloproteinase-1 (MMP-1) expression was lower in UVB + 20% RE group compared to the UVB group (p<0.05), however the MMP1 expression in UVB + 40% RE group was higher than other treatment group.
CONCLUSION: RE cream prevents wrinkle by maintaining epidermal thickness and collagen contain. RE cream also decreases MMP-1 expression in mice.
KEYWORDS: coffee, collagen, MMP-1, robusta, wrinkle |
| first_indexed | 2024-12-22T20:54:13Z |
| format | Article |
| id | doaj.art-aa72f2b3016643cdbe7e55b24fda75b6 |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-12-22T20:54:13Z |
| publishDate | 2021-03-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-aa72f2b3016643cdbe7e55b24fda75b62022-12-21T18:12:59ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792021-03-01131849010.18585/inabj.v13i1.1428397Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1Dimpuulina Erna Mariati0Sunarjati Sudigdoadi1Ronny Lesmana2Astrid Feinisa Khairani3Julia Windi Gunadi4Vita Murniati Tarawan5Unang Supratman6Hanna Goenawan7Magister Program of Anti Aging, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Microbiology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Physiology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Cell Biology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Department of Physiology, Faculty of Medicine, Maranatha Christian University, Jl. Profesor drg. Suria Sumantri, M.P.H. No. 65, Bandung 40164Physiology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363Physiology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor 45363BACKGROUND: Recently, coffee is widely used for preventing photoaging because of its antioxidant capacity. Among two kinds of coffee, robusta coffee has higher content of antioxidant such as chlorogenic acid (CGA) and caffeine. Researchs about robusta coffee bean effect on photoaging due to UVB radiation is still limited. Therefore, the aim of this study was to examine the effect of robusta extract cream (RE cream) on preventing wrinkle in mice induced by ultraviolet-B (UVB) radiation. METHODS: RE cream was made by mixing RE coffee with moisturizing cream in different concentration (10%, 20%, and 40%). Twenty-five male of Mus musculus Balb/c strain mice aged 4 weeks were divided into five groups; control group, UVB group, UVB + 10% RE group, UVB + 20% RE group, and UVB + 40% RE group. The UVB groups were given UVB radiation three times a week with an exposure duration of 100 seconds per time for ten weeks. At the end of the treatment, skin samples were excised and statined histologically, also were analyzed for their protein expression. Evaluation of wrinkles was carried out using the Bissete method before and after treatment. To evaluate the thickness of the epidermis, HE staining was performed, while masson Trichome staining was performed to determine the collagen content. RESULTS: RE cream-treated groups showed lower wrinkle score compared to the control group. Furthermore, in UVB + 10% RE group, the RE cream application reduce wrinkle formation. In UVB + 10% RE group and UVB + 20% RE group, the RE cream application increased epidermal thickness and collagen content (p=0.00). While collagenase, matrix metalloproteinase-1 (MMP-1) expression was lower in UVB + 20% RE group compared to the UVB group (p<0.05), however the MMP1 expression in UVB + 40% RE group was higher than other treatment group. CONCLUSION: RE cream prevents wrinkle by maintaining epidermal thickness and collagen contain. RE cream also decreases MMP-1 expression in mice. KEYWORDS: coffee, collagen, MMP-1, robusta, wrinklehttps://inabj.org/index.php/ibj/article/view/1428 |
| spellingShingle | Dimpuulina Erna Mariati Sunarjati Sudigdoadi Ronny Lesmana Astrid Feinisa Khairani Julia Windi Gunadi Vita Murniati Tarawan Unang Supratman Hanna Goenawan Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 Indonesian Biomedical Journal |
| title | Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 |
| title_full | Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 |
| title_fullStr | Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 |
| title_full_unstemmed | Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 |
| title_short | Robusta Extract Cream Ameliorated Ultraviolet B-induced Wrinkle Skin of Mice by the Regulation of Epidermal Thickness and Inhibition of MMP-1 |
| title_sort | robusta extract cream ameliorated ultraviolet b induced wrinkle skin of mice by the regulation of epidermal thickness and inhibition of mmp 1 |
| url | https://inabj.org/index.php/ibj/article/view/1428 |
| work_keys_str_mv | AT dimpuulinaernamariati robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT sunarjatisudigdoadi robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT ronnylesmana robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT astridfeinisakhairani robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT juliawindigunadi robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT vitamurniatitarawan robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT unangsupratman robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 AT hannagoenawan robustaextractcreamamelioratedultravioletbinducedwrinkleskinofmicebytheregulationofepidermalthicknessandinhibitionofmmp1 |